ID   VKT1_ARAVE              Reviewed;          80 AA.
AC   Q8T3S7;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Kunitz-type U1-aranetoxin-Av1a;
DE            Short=U1-AATX-Av1a;
DE   AltName: Full=Toxin 1;
DE            Short=AvTox-1;
OS   Araneus ventricosus (Orbweaver spider) (Epeira ventricosa).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Araneoidea; Araneidae; Araneus.
OX   NCBI_TaxID=182803;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Jung E.H., Lee K.S., Han J.H., Je Y.H., Chang J.H., Roh J.Y., Sohn H.D.,
RA   Jin B.R.;
RT   "Molecular cloning of two cDNAs encoding an insecticidal toxin from the
RT   spider, Araneus ventricosus, and construction of a recombinant baculovirus
RT   expressing a spider toxin.";
RL   Int. J. Ind. Entomol. 4:43-49(2002).
CC   -!- FUNCTION: Putative insecticidal toxin that may inhibit trypsin.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- MISCELLANEOUS: Since this peptide has no sequence signal, its secretion
CC       is unsure.
CC   -!- SIMILARITY: Belongs to the venom Kunitz-type family. 02 (native)
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AY091482; AAM14403.1; -; mRNA.
DR   AlphaFoldDB; Q8T3S7; -.
DR   SMR; Q8T3S7; -.
DR   MEROPS; I02.964; -.
DR   ArachnoServer; AS000114; U1-aranetoxin-Av1a.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044562; P:envenomation resulting in negative regulation of voltage-gated potassium channel activity in another organism; IEA:UniProt.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Protease inhibitor; Serine protease inhibitor; Toxin.
FT   CHAIN           1..80
FT                   /note="Kunitz-type U1-aranetoxin-Av1a"
FT                   /id="PRO_0000380153"
FT   DOMAIN          27..77
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   SITE            47..48
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000250"
FT   DISULFID        27..77
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        36..60
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        52..73
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   80 AA;  9275 MW;  D07F5F9AC6B7BC10 CRC64;
     MPFHYFPKLN PITWIPGWIP GLGKDRCLLP KVTGPCKASL TRYYYDKDTK ACVEFIYGGC
     RGNRNNFKRK DECEKACTDH