ID   CALB_PSEAE              Reviewed;         476 AA.
AC   Q9I6C8;
DT   11-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Probable coniferyl aldehyde dehydrogenase;
DE            Short=CALDH;
DE            EC=1.2.1.68;
GN   Name=calB; OrderedLocusNames=PA0366;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(E)-coniferaldehyde + H2O + NADP(+) = (E)-ferulate + 2 H(+) +
CC         NADPH; Xref=Rhea:RHEA:23964, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16547, ChEBI:CHEBI:29749, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.2.1.68;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(E)-coniferaldehyde + H2O + NAD(+) = (E)-ferulate + 2 H(+) +
CC         NADH; Xref=Rhea:RHEA:23968, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16547, ChEBI:CHEBI:29749, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.2.1.68;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; AE004091; AAG03755.1; -; Genomic_DNA.
DR   PIR; D83600; D83600.
DR   RefSeq; NP_249057.1; NC_002516.2.
DR   RefSeq; WP_003102868.1; NZ_QZGE01000016.1.
DR   AlphaFoldDB; Q9I6C8; -.
DR   SMR; Q9I6C8; -.
DR   STRING; 287.DR97_3332; -.
DR   PaxDb; Q9I6C8; -.
DR   PRIDE; Q9I6C8; -.
DR   EnsemblBacteria; AAG03755; AAG03755; PA0366.
DR   GeneID; 880299; -.
DR   KEGG; pae:PA0366; -.
DR   PATRIC; fig|208964.12.peg.385; -.
DR   PseudoCAP; PA0366; -.
DR   HOGENOM; CLU_005391_3_6_6; -.
DR   InParanoid; Q9I6C8; -.
DR   OMA; RHGKRWM; -.
DR   PhylomeDB; Q9I6C8; -.
DR   BioCyc; PAER208964:G1FZ6-369-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004029; F:aldehyde dehydrogenase (NAD+) activity; IBA:GO_Central.
DR   GO; GO:0050269; F:coniferyl-aldehyde dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006081; P:cellular aldehyde metabolic process; IBA:GO_Central.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   InterPro; IPR012394; Aldehyde_DH_NAD(P).
DR   PANTHER; PTHR43570; PTHR43570; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   PIRSF; PIRSF036492; ALDH; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..476
FT                   /note="Probable coniferyl aldehyde dehydrogenase"
FT                   /id="PRO_0000056587"
FT   ACT_SITE        225
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
FT   ACT_SITE        259
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
SQ   SEQUENCE   476 AA;  52876 MW;  9C4A0B4D3E334BDD CRC64;
     MVADIAYLQQ SQQEINQLEA LFARQRAAYL AQPMPDATQR VQWLKALRDL LFKEQQALIE
     AIDRDFSARS ADETLLAEIM PSLHGIHYAA KRVKKWMKPA RRAVGLQFQP ASAQVVYQPL
     GVVGVIVPWN YPLFLSIGPL TGALAAGNRV MIKMSESTPA TGRLLKDLLA RIFPEDQVAV
     VLGEVDVGVA FSKLPFDHLL FTGATSVGKH VMRAAAENLT PVTLELGGKS PAIVSDSVPM
     KDAAERIAFG KSLNAGQTCV APDYVLVPSR RVEEFVSQYK EVVQGFFPRL SDNPDYTAII
     NERQLGRLRG YLDDAREKGA TLVPLFAEGQ QRRLPQTLLL NVSDDMKVMQ EEIFGPLLPV
     IPYERLEDAL AYVNQRPRPL ALYYFGYDKA QQQRVLHETH SGGVCLNDTL LHVAQDDIPF
     GGVGPSGMGH YHGHEGFLTF SKAKGVFSKP RFNAARMIYP PYGKSIQKLV YKLFVR