ID   VKT2_NAJNI              Reviewed;          57 AA.
AC   P00986;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Kunitz-type serine protease inhibitor 2;
DE   AltName: Full=Venom basic protease inhibitor 2;
DE   AltName: Full=Venom basic protease inhibitor II;
OS   Naja nivea (Cape cobra) (Coluber niveus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8655;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Venom;
RX   PubMed=950337; DOI=10.1093/oxfordjournals.jbchem.a131100;
RA   Hokama Y., Iwanaga S., Tatsuki T., Suzuki T.;
RT   "Snake venom proteinase inhibitors. III. Isolation of five polypeptide
RT   inhibitors from the venoms of Hemachatus haemachatus (Ringhal's corbra) and
RT   Naja nivea (Cape cobra) and the complete amino acid sequences of two of
RT   them.";
RL   J. Biochem. 79:559-578(1976).
CC   -!- FUNCTION: Serine protease inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- SIMILARITY: Belongs to the venom Kunitz-type family. {ECO:0000305}.
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DR   PIR; A01217; TINJVC.
DR   AlphaFoldDB; P00986; -.
DR   SMR; P00986; -.
DR   MEROPS; I02.055; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor.
FT   CHAIN           1..57
FT                   /note="Kunitz-type serine protease inhibitor 2"
FT                   /id="PRO_0000155441"
FT   DOMAIN          5..55
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   SITE            15..16
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000250"
FT   DISULFID        5..55
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        14..38
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        30..51
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   57 AA;  6466 MW;  7CA69230A02887D1 CRC64;
     RPRFCELPAE TGLCKARIRS FHYNRAAQQC LEFIYGGCGG NANRFKTIDE CHRTCVG