VKT4_CYRSC
ID VKT4_CYRSC Reviewed; 88 AA.
AC P0DJ76; A0A023WBH6;
DT 16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 03-AUG-2022, entry version 31.
DE RecName: Full=Kunitz-type kappaPI-theraphotoxin-Hs1e;
DE Short=KappaPI-TRTX-Hs1e;
DE AltName: Full=Huwentoxin HW11c4 {ECO:0000303|PubMed:24418069};
DE AltName: Full=Kunitz-type serine protease inhibitor HWTX-XI-IS4 {ECO:0000303|PubMed:18923708};
DE Flags: Precursor;
OS Cyriopagopus schmidti (Chinese bird spider) (Haplopelma schmidti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Mygalomorphae; Theraphosidae; Cyriopagopus.
OX NCBI_TaxID=29017;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=18923708; DOI=10.1371/journal.pone.0003414;
RA Yuan C.-H., He Q.-Y., Peng K., Diao J.-B., Jiang L.-P., Tang X.,
RA Liang S.-P.;
RT "Discovery of a distinct superfamily of Kunitz-type toxin (KTT) from
RT tarantulas.";
RL PLoS ONE 3:E3414-E3414(2008).
RN [2] {ECO:0000312|EMBL:AHY30306.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND RECOMBINANT EXPRESSION.
RX PubMed=24418069; DOI=10.1016/j.peptides.2014.01.001;
RA Jiang L., Deng M., Duan Z., Tang X., Liang S.;
RT "Molecular cloning, bioinformatics analysis and functional characterization
RT of HWTX-XI toxin superfamily from the spider Ornithoctonus huwena.";
RL Peptides 54:9-18(2014).
CC -!- FUNCTION: Toxin that shows weak activity against voltage-gated
CC potassium channels (Kv), and no activity against serine proteases
CC (PubMed:24418069). The recombinant toxin shows a weak inhibition on
CC Kv1.1/KCNA1 (59.2% inhibition at 10 uM) (PubMed:24418069).
CC {ECO:0000269|PubMed:24418069}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:18923708}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:18923708}.
CC -!- MISCELLANEOUS: The recombinant toxin does not show activity on
CC Kv1.2/KCNA2, Kv1.3/KCNA3, Kv2.1/KCNB1 and Kv4.3/KCND3 channels, as well
CC as on calcium (Cav) and sodium channels (Nav). It also does not show
CC detectable activity against trypsin, kallikrein, chymotrypsin, and
CC thrombin. {ECO:0000269|PubMed:24418069}.
CC -!- SIMILARITY: Belongs to the venom Kunitz-type family. 02 (native)
CC subfamily. {ECO:0000305}.
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DR EMBL; KF160295; AHY30306.1; -; Genomic_DNA.
DR AlphaFoldDB; P0DJ76; -.
DR SMR; P0DJ76; -.
DR ArachnoServer; AS001843; kappa-theraphotoxin-Hs1e.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0044562; P:envenomation resulting in negative regulation of voltage-gated potassium channel activity in another organism; IEA:UniProt.
DR CDD; cd00109; KU; 1.
DR Gene3D; 4.10.410.10; -; 1.
DR InterPro; IPR002223; Kunitz_BPTI.
DR InterPro; IPR036880; Kunitz_BPTI_sf.
DR InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR Pfam; PF00014; Kunitz_BPTI; 1.
DR PRINTS; PR00759; BASICPTASE.
DR SMART; SM00131; KU; 1.
DR SUPFAM; SSF57362; SSF57362; 1.
DR PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Ion channel impairing toxin;
KW Potassium channel impairing toxin; Protease inhibitor; Secreted;
KW Serine protease inhibitor; Signal; Toxin;
KW Voltage-gated potassium channel impairing toxin.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT PROPEP 28..33
FT /evidence="ECO:0000250"
FT /id="PRO_0000413826"
FT CHAIN 34..88
FT /note="Kunitz-type kappaPI-theraphotoxin-Hs1e"
FT /id="PRO_0000413827"
FT DOMAIN 37..85
FT /note="BPTI/Kunitz inhibitor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT SITE 39
FT /note="May bind Kv1.x/KCNA"
FT /evidence="ECO:0000250"
FT SITE 47..48
FT /note="Reactive bond for trypsin"
FT /evidence="ECO:0000250"
FT DISULFID 37..85
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT DISULFID 46..68
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT DISULFID 60..81
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ SEQUENCE 88 AA; 9747 MW; 1A86EB72DDD4E94D CRC64;
MGIARILSAV LFLSVLFVVT FPALLLADHH DGRIDTCRLP SDRGRCKASF ERWYFNGRTC
AKFIYGGCGG NGNKFPTEKA CMKRCAKA
