ID   VKT4_WALAE              Reviewed;          83 AA.
AC   C1IC53;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Protease inhibitor 4;
DE   AltName: Full=Kunitz inhibitor IV;
DE   Flags: Precursor;
OS   Walterinnesia aegyptia (Desert black snake).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Walterinnesia.
OX   NCBI_TaxID=64182;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-40, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=18405934; DOI=10.1016/j.toxicon.2008.02.012;
RA   Tsai H.-Y., Wang Y.M., Tsai I.-H.;
RT   "Cloning, characterization and phylogenetic analyses of members of three
RT   major venom families from a single specimen of Walterinnesia aegyptia.";
RL   Toxicon 51:1245-1254(2008).
CC   -!- FUNCTION: Snake venom serine protease inhibitor. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- MASS SPECTROMETRY: Mass=6779; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:18405934};
CC   -!- SIMILARITY: Belongs to the venom Kunitz-type family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EU196561; ABX82870.1; -; mRNA.
DR   AlphaFoldDB; C1IC53; -.
DR   SMR; C1IC53; -.
DR   MEROPS; I02.022; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor; Signal; Toxin.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000269|PubMed:18405934"
FT   CHAIN           25..83
FT                   /note="Protease inhibitor 4"
FT                   /id="PRO_0000419230"
FT   DOMAIN          31..81
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        31..81
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        40..64
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        56..77
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   83 AA;  9270 MW;  B3D0399DDBB94C6A CRC64;
     MSSGCLLLLL GLLTLWAELT PISGLGGPKY CHLPADPGPC SNYRPAYYYN PASRKCEEFM
     YGGCKGNKNN FKTRHECHRV CVR