ID   VKTB1_CONBU             Reviewed;          88 AA.
AC   P0CY85;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Kunitz-type conkunitzin-B1;
DE            Short=Conk-B1;
DE   Flags: Precursor;
OS   Conus bullatus (Bubble cone).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC   Caenogastropoda; Neogastropoda; Conoidea; Conidae; Conus; Textilia.
OX   NCBI_TaxID=89438;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom duct;
RX   PubMed=21266071; DOI=10.1186/1471-2164-12-60;
RA   Hu H., Bandyopadhyay P.K., Olivera B.M., Yandell M.;
RT   "Characterization of the Conus bullatus genome and its venom-duct
RT   transcriptome.";
RL   BMC Genomics 12:60-60(2011).
CC   -!- FUNCTION: Blocks specifically voltage-activated potassium channels (Kv)
CC       of the Shaker family. {ECO:0000250|UniProtKB:P0C1X2}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:21266071}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom duct.
CC       {ECO:0000305|PubMed:21266071}.
CC   -!- PTM: Contains 2 disulfide bonds instead of 3, as for all Kunitz domain
CC       proteins.
CC   -!- SIMILARITY: Belongs to the venom Kunitz-type family. {ECO:0000305}.
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DR   AlphaFoldDB; P0CY85; -.
DR   SMR; P0CY85; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   3: Inferred from homology;
KW   Amidation; Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Potassium channel impairing toxin; Protease inhibitor; Secreted;
KW   Serine protease inhibitor; Signal; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..87
FT                   /note="Kunitz-type conkunitzin-B1"
FT                   /id="PRO_0000409987"
FT   DOMAIN          33..83
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   MOD_RES         87
FT                   /note="Proline amide"
FT                   /evidence="ECO:0000250"
FT   DISULFID        33..83
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        58..79
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   88 AA;  9931 MW;  2924E233616677C7 CRC64;
     MEGRRFAAVL ILPICMLAPG AVASKRWTRP SVCNLPAESG TGTQSLKRFY YNSDKMQCRT
     FIYKGNGGND NNFPRTYDCQ KKCLYRPG