CALCB_HUMAN
ID CALCB_HUMAN Reviewed; 127 AA.
AC P10092; A8K573; D3DQX4; Q569I0; Q9UCN9;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Calcitonin gene-related peptide 2;
DE AltName: Full=Beta-type CGRP;
DE Short=Beta-CGRP;
DE AltName: Full=Calcitonin gene-related peptide II;
DE Short=CGRP-II;
DE Flags: Precursor;
GN Name=CALCB; Synonyms=CALC2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3492393; DOI=10.1016/0014-5793(86)81091-2;
RA Steenbergh P.H., Hoeppener J.W.M., Zandberg J., Visser A., Lips C.J.M.,
RA Jansz H.S.;
RT "Structure and expression of the human calcitonin/CGRP genes.";
RL FEBS Lett. 209:97-103(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 56-127.
RX PubMed=2985435; DOI=10.1016/0014-5793(85)80820-6;
RA Steenbergh P.H., Hoeppener J.W.M., Zandberg J., Lips C.J.M., Jansz H.S.;
RT "A second human calcitonin/CGRP gene.";
RL FEBS Lett. 183:403-407(1985).
RN [6]
RP PROTEIN SEQUENCE OF 82-108.
RX PubMed=3492492; DOI=10.1016/s0021-9258(19)75816-5;
RA Petermann J.B., Born W., Chang J.Y., Fischer J.A.;
RT "Identification in the human central nervous system, pituitary, and thyroid
RT of a novel calcitonin gene-related peptide, and partial amino acid sequence
RT in the spinal cord.";
RL J. Biol. Chem. 262:542-545(1987).
RN [7]
RP PROTEIN SEQUENCE OF 82-86 AND 104-118, AMIDATION AT PHE-118, AND DISULFIDE
RP BOND.
RC TISSUE=Spinal cord;
RX PubMed=2322288; DOI=10.1016/0006-291x(90)90621-s;
RA Wimalawansa S.J., Morris H.R., Etienne A., Blench I., Panico M.,
RA McIntyre I.;
RT "Isolation, purification and characterization of beta-hCGRP from human
RT spinal cord.";
RL Biochem. Biophys. Res. Commun. 167:993-1000(1990).
RN [8]
RP PROTEIN SEQUENCE OF 82-104.
RC TISSUE=Pheochromocytoma;
RX PubMed=1318039; DOI=10.1016/s0006-291x(05)80966-0;
RA Kitamura K., Kangawa K., Kawamoto M., Ichiki Y., Matsuo H., Eto T.;
RT "Isolation and characterization of peptides which act on rat platelets,
RT from a pheochromocytoma.";
RL Biochem. Biophys. Res. Commun. 185:134-141(1992).
CC -!- FUNCTION: CGRP induces vasodilation. It dilates a variety of vessels
CC including the coronary, cerebral and systemic vasculature. Its
CC abundance in the CNS also points toward a neurotransmitter or
CC neuromodulator role.
CC -!- INTERACTION:
CC P10092; Q99828: CIB1; NbExp=3; IntAct=EBI-12883326, EBI-372594;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the calcitonin family. {ECO:0000305}.
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DR EMBL; X04855; CAC05295.1; -; Genomic_DNA.
DR EMBL; X04857; CAC05295.1; JOINED; Genomic_DNA.
DR EMBL; X04861; CAC05295.1; JOINED; Genomic_DNA.
DR EMBL; AK291188; BAF83877.1; -; mRNA.
DR EMBL; CH471064; EAW68466.1; -; Genomic_DNA.
DR EMBL; CH471064; EAW68467.1; -; Genomic_DNA.
DR EMBL; BC092468; AAH92468.1; -; mRNA.
DR EMBL; X02404; CAA26249.1; -; mRNA.
DR CCDS; CCDS7820.1; -.
DR PIR; A25864; A25864.
DR PIR; I37232; I37232.
DR RefSeq; NP_000719.1; NM_000728.3.
DR AlphaFoldDB; P10092; -.
DR BMRB; P10092; -.
DR BioGRID; 107248; 10.
DR IntAct; P10092; 2.
DR STRING; 9606.ENSP00000433490; -.
DR ChEMBL; CHEMBL3832947; -.
DR DrugBank; DB14040; Eptinezumab.
DR DrugBank; DB14041; Fremanezumab.
DR DrugBank; DB14042; Galcanezumab.
DR DrugBank; DB12228; Telcagepant.
DR DrugCentral; P10092; -.
DR iPTMnet; P10092; -.
DR PhosphoSitePlus; P10092; -.
DR BioMuta; CALCB; -.
DR DMDM; 115487; -.
DR jPOST; P10092; -.
DR MassIVE; P10092; -.
DR PaxDb; P10092; -.
DR PeptideAtlas; P10092; -.
DR PRIDE; P10092; -.
DR ProteomicsDB; 52565; -.
DR ABCD; P10092; 3 sequenced antibodies.
DR Antibodypedia; 24714; 76 antibodies from 15 providers.
DR DNASU; 797; -.
DR Ensembl; ENST00000324229.11; ENSP00000346017.5; ENSG00000175868.14.
DR Ensembl; ENST00000533448.1; ENSP00000433490.1; ENSG00000175868.14.
DR GeneID; 797; -.
DR KEGG; hsa:797; -.
DR MANE-Select; ENST00000324229.11; ENSP00000346017.5; NM_000728.4; NP_000719.1.
DR UCSC; uc001mlx.2; human.
DR CTD; 797; -.
DR DisGeNET; 797; -.
DR GeneCards; CALCB; -.
DR HGNC; HGNC:1438; CALCB.
DR HPA; ENSG00000175868; Group enriched (brain, thyroid gland).
DR MIM; 114160; gene.
DR neXtProt; NX_P10092; -.
DR OpenTargets; ENSG00000175868; -.
DR PharmGKB; PA26030; -.
DR VEuPathDB; HostDB:ENSG00000175868; -.
DR eggNOG; ENOG502SQMP; Eukaryota.
DR GeneTree; ENSGT00940000156267; -.
DR HOGENOM; CLU_122444_1_0_1; -.
DR InParanoid; P10092; -.
DR OMA; VKDFVQM; -.
DR OrthoDB; 1547206at2759; -.
DR PhylomeDB; P10092; -.
DR TreeFam; TF333069; -.
DR PathwayCommons; P10092; -.
DR Reactome; R-HSA-418555; G alpha (s) signalling events.
DR Reactome; R-HSA-419812; Calcitonin-like ligand receptors.
DR Reactome; R-HSA-9660821; ADORA2B mediated anti-inflammatory cytokines production.
DR SignaLink; P10092; -.
DR BioGRID-ORCS; 797; 20 hits in 1057 CRISPR screens.
DR ChiTaRS; CALCB; human.
DR GenomeRNAi; 797; -.
DR Pharos; P10092; Tclin.
DR PRO; PR:P10092; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; P10092; protein.
DR Bgee; ENSG00000175868; Expressed in dorsal root ganglion and 92 other tissues.
DR ExpressionAtlas; P10092; baseline and differential.
DR Genevisible; P10092; HS.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0031716; F:calcitonin receptor binding; IBA:GO_Central.
DR GO; GO:0005184; F:neuropeptide hormone activity; TAS:ProtInc.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; TAS:ProtInc.
DR GO; GO:0051480; P:regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR InterPro; IPR021117; Calcitonin-like.
DR InterPro; IPR021116; Calcitonin/adrenomedullin.
DR InterPro; IPR018360; Calcitonin_CS.
DR InterPro; IPR015476; Calcitonin_gene-rel_peptide.
DR InterPro; IPR001693; Calcitonin_peptide-like.
DR PANTHER; PTHR10505; PTHR10505; 1.
DR Pfam; PF00214; Calc_CGRP_IAPP; 1.
DR PRINTS; PR00817; CALCITONINB.
DR SMART; SM00113; CALCITONIN; 1.
DR PROSITE; PS00258; CALCITONIN; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Disulfide bond; Hormone; Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT PROPEP 26..79
FT /id="PRO_0000004086"
FT PEPTIDE 82..118
FT /note="Calcitonin gene-related peptide 2"
FT /id="PRO_0000004087"
FT PROPEP 124..127
FT /id="PRO_0000004088"
FT MOD_RES 118
FT /note="Phenylalanine amide"
FT /evidence="ECO:0000269|PubMed:2322288"
FT DISULFID 83..88
FT /evidence="ECO:0000269|PubMed:2322288"
FT CONFLICT 73
FT /note="G -> S (in Ref. 5; CAA26249)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 127 AA; 13706 MW; B0A71A063CD5ACE7 CRC64;
MGFRKFSPFL ALSILVLYQA GSLQAAPFRS ALESSPDPAT LSKEDARLLL AALVQDYVQM
KASELKQEQE TQGSSSAAQK RACNTATCVT HRLAGLLSRS GGMVKSNFVP TNVGSKAFGR
RRRDLQA
