ID   VKT_VIPBN               Reviewed;          95 AA.
AC   E5AJX3;
DT   11-JUN-2014, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Kunitz-type serine protease inhibitor ki-VN;
DE   Flags: Precursor;
OS   Vipera berus nikolskii (Nikolsky's adder) (Vipera nikolskii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Viperinae; Vipera.
OX   NCBI_TaxID=1808362;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=21899053; DOI=10.1134/s1068162011030149;
RA   Ramazanova A.S., Fil'kin S.I., Starkov V.G., Utkin I.N.;
RT   "Molecular cloning and analysis of cDNA sequences encoding serine
RT   proteinase and Kunitz type inhibitor in venom gland of Vipera nikolskii
RT   viper.";
RL   Bioorg. Khim. 37:374-385(2011).
CC   -!- FUNCTION: Serine protease inhibitor. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- SIMILARITY: Belongs to the venom Kunitz-type family. {ECO:0000305}.
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DR   EMBL; FR669450; CBW30779.1; -; mRNA.
DR   AlphaFoldDB; E5AJX3; -.
DR   SMR; E5AJX3; -.
DR   MEROPS; I02.062; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Protease inhibitor; Secreted; Serine protease inhibitor;
KW   Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..91
FT                   /note="Kunitz-type serine protease inhibitor ki-VN"
FT                   /id="PRO_5000666459"
FT   PROPEP          92..95
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_5000666460"
FT   DOMAIN          31..81
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   SITE            41..42
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   DISULFID        31..81
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        40..64
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        56..77
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   95 AA;  10426 MW;  0C2B260DA5E39674 CRC64;
     MSSGGLLLLL GLLTLWAELT PVSSRDRPKF CYLPAEPGEC NAYMPSFYYD SASNKCKKFI
     YGGCRGNANN FKTRDECHHT CVASGKGIQP RIGSN