ID   VLD1_YEAST              Reviewed;         242 AA.
AC   P40570; D6VVU4;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 2.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=Vacuole localized DSC protein 1 {ECO:0000303|PubMed:29355480};
GN   Name=VLD1 {ECO:0000303|PubMed:29355480}; OrderedLocusNames=YIR014W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169870;
RA   Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA   Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA   Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA   Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA   Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL   Nature 387:84-87(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   IDENTIFICATION OF PROBABLE INITIATION SITE.
RX   PubMed=12748633; DOI=10.1038/nature01644;
RA   Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT   "Sequencing and comparison of yeast species to identify genes and
RT   regulatory elements.";
RL   Nature 423:241-254(2003).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, AND IDENTIFICATION IN THE VACUOLE-LOCALIZED
RP   DSC COMPLEX.
RX   PubMed=29355480; DOI=10.7554/elife.33116;
RA   Yang X., Arines F.M., Zhang W., Li M.;
RT   "Sorting of a multi-subunit ubiquitin ligase complex in the endolysosome
RT   system.";
RL   Elife 7:0-0(2018).
CC   -!- FUNCTION: Component of the vacuole-localized DSC E3 ubiquitin ligase
CC       complex involved in the targeting of the complex to the vacuole
CC       membrane via the AP3 pathway to ubiquinate vacuolar membrane proteins
CC       (PubMed:29355480). Competes with GLD1 to determine the subcellular
CC       localizations of the DSC complex (PubMed:29355480).
CC       {ECO:0000269|PubMed:29355480}.
CC   -!- SUBUNIT: Part of the vacuole-localized DSC E3 ligase complex composed
CC       of at least TUL1, DSC2, DSC3, UBX3, CDC48 and VLD1.
CC       {ECO:0000269|PubMed:29355480}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:29355480};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAT92745.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA86084.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; Z37996; CAA86084.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AY692726; AAT92745.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BK006942; DAA08560.1; -; Genomic_DNA.
DR   PIR; S48358; S48358.
DR   RefSeq; NP_012279.4; NM_001179536.3.
DR   AlphaFoldDB; P40570; -.
DR   BioGRID; 35006; 38.
DR   DIP; DIP-2640N; -.
DR   IntAct; P40570; 3.
DR   MINT; P40570; -.
DR   STRING; 4932.YIR014W; -.
DR   MaxQB; P40570; -.
DR   PaxDb; P40570; -.
DR   PRIDE; P40570; -.
DR   EnsemblFungi; YIR014W_mRNA; YIR014W; YIR014W.
DR   GeneID; 854831; -.
DR   KEGG; sce:YIR014W; -.
DR   SGD; S000001453; YIR014W.
DR   VEuPathDB; FungiDB:YIR014W; -.
DR   eggNOG; ENOG502S7ZS; Eukaryota.
DR   HOGENOM; CLU_098038_0_0_1; -.
DR   InParanoid; P40570; -.
DR   OMA; CQIVIIN; -.
DR   BioCyc; YEAST:G3O-31435-MON; -.
DR   PRO; PR:P40570; -.
DR   Proteomes; UP000002311; Chromosome IX.
DR   RNAct; P40570; protein.
DR   GO; GO:0044695; C:Dsc E3 ubiquitin ligase complex; IDA:SGD.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0071627; C:integral component of fungal-type vacuolar membrane; ISM:SGD.
DR   GO; GO:0031503; P:protein-containing complex localization; IMP:SGD.
PE   1: Evidence at protein level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix; Vacuole.
FT   CHAIN           1..242
FT                   /note="Vacuole localized DSC protein 1"
FT                   /id="PRO_0000203004"
FT   TRANSMEM        128..148
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   242 AA;  27600 MW;  D81202C770462809 CRC64;
     MLHLEDDNGR QRSVIANLQK FVYCCLYLRF IKDGSLFLIL LGWIISSLCD FIQELTLRYL
     KKNYLEVGRD NDQEDDESLA IRGLETPIVR MIINKAIRYY QGLILLETAY CIVYHIRLDV
     SRDICSKPYG FVIMLLIREF TCPVPTAFPS KLLLVLLDIL LLFCQIVIIN GSLSSSLQNV
     KLIVKELNAE EEGALNILKL NTWHMDATGP ELIVLKNHDK SIPQQADGDD ATEITPLLNI
     AE