ID   VLF1_NPVAC              Reviewed;         379 AA.
AC   Q06687;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Very late expression factor 1;
GN   Name=VLF-1;
OS   Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=46015;
OH   NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C6;
RX   PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA   Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT   "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT   virus.";
RL   Virology 202:586-605(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=E2;
RX   PubMed=8126447; DOI=10.1099/0022-1317-75-3-487;
RA   Kool M., Broer R., Zuidema D., Goldbach R.W., Vlak J.M.;
RT   "Nucleotide sequence and genetic organization of a 7.3 kb region (map unit
RT   47 to 52.5) of Autographa californica nuclear polyhedrosis virus fragment
RT   EcoRI-C.";
RL   J. Gen. Virol. 75:487-494(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=L1;
RX   PubMed=7966564; DOI=10.1128/jvi.68.12.7746-7756.1994;
RA   McLachlin J.R., Miller L.K.;
RT   "Identification and characterization of vlf-1, a baculovirus gene involved
RT   in very late gene expression.";
RL   J. Virol. 68:7746-7756(1994).
RN   [4]
RP   FUNCTION.
RX   PubMed=12350233; DOI=10.1186/1471-2199-3-14;
RA   Mikhailov V.S., Rohrmann G.F.;
RT   "Binding of the baculovirus very late expression factor 1 (VLF-1) to
RT   different DNA structures.";
RL   BMC Mol. Biol. 3:14-14(2002).
RN   [5]
RP   FUNCTION.
RX   PubMed=16175333; DOI=10.1007/s11262-005-3242-3;
RA   Li Y., Wang J., Deng R., Zhang Q., Yang K., Wang X.;
RT   "vlf-1 deletion brought AcMNPV to defect in nucleocapsid formation.";
RL   Virus Genes 31:275-284(2005).
RN   [6]
RP   FUNCTION.
RX   PubMed=16439529; DOI=10.1128/jvi.80.4.1724-1733.2006;
RA   Vanarsdall A.L., Okano K., Rohrmann G.F.;
RT   "Characterization of the role of very late expression factor 1 in
RT   baculovirus capsid structure and DNA processing.";
RL   J. Virol. 80:1724-1733(2006).
CC   -!- FUNCTION: Plays a role in nucleocapsid assembly and serves an essential
CC       function during the final stages of the DNA packaging process.
CC       Participates in the processing of branched DNA molecules at the late
CC       stages of viral genome replication. {ECO:0000269|PubMed:12350233,
CC       ECO:0000269|PubMed:16175333, ECO:0000269|PubMed:16439529}.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU01246}.
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DR   EMBL; L22858; AAA66707.1; -; Genomic_DNA.
DR   EMBL; X71415; CAA50540.1; -; Genomic_DNA.
DR   EMBL; L35905; AAA62646.1; -; Genomic_DNA.
DR   PIR; F72859; F72859.
DR   RefSeq; NP_054107.1; NC_001623.1.
DR   PRIDE; Q06687; -.
DR   GeneID; 1403910; -.
DR   KEGG; vg:1403910; -.
DR   Proteomes; UP000008292; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:InterPro.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.443.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat_sf.
DR   InterPro; IPR002104; Integrase_catalytic.
DR   Pfam; PF00589; Phage_integrase; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE   1: Evidence at protein level;
KW   DNA recombination; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..379
FT                   /note="Very late expression factor 1"
FT                   /id="PRO_0000132875"
FT   DOMAIN          169..348
FT                   /note="Tyr recombinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   REGION          346..379
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        363..379
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        210
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        239
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        303
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        326
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT   ACT_SITE        335
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
SQ   SEQUENCE   379 AA;  44364 MW;  81322F8B403869A0 CRC64;
     MNGFNVRNEN NFNSWKIKIQ SAPRFESVFD LATDRQRCTP DEVKNNSLWS KYMFPKPFAP
     TTLKSYKSRF IKIVYCSVDD VHLEDMSYSL DKEFDSIENQ TLLIDPQELC RRMLELRSVT
     KETLQLTINF YTNMMNLPEY KIPRMVMLPR DKELKNIREK EKNLMLKNVI DTILNFINDK
     IKMLNSDYVH DRGLIRGAIV FCIMLGTGMR INEARQLSVD DLNVLIKRGK LHSDTINLKR
     KRSRNNTLNN IKMKPLELAR EIYSRNPTIL QISKNTSTPF KDFRRLLEES GVEMERPRSN
     MIRHYLSSNL YNSGVPLQKV AKLMNHESSA STKHYLNKYN IGLDETSSEE ENNNDDDDAQ
     HNRNSSGSSG ESLLYYRNE