VLMS_LECSP
ID VLMS_LECSP Reviewed; 8903 AA.
AC A0A024F910;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 09-JUL-2014, sequence version 1.
DT 23-FEB-2022, entry version 24.
DE RecName: Full=Nonribosomal peptide synthetase vlms {ECO:0000303|PubMed:24848421};
DE Short=NRPS vlmS {ECO:0000303|PubMed:24848421};
DE EC=6.3.2.- {ECO:0000305|PubMed:24848421};
DE AltName: Full=Verlamelin biosynthesis protein S {ECO:0000303|PubMed:24848421};
GN Name=vlmS {ECO:0000303|PubMed:24848421};
OS Lecanicillium sp.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Cordycipitaceae; Lecanicillium;
OC unclassified Lecanicillium.
OX NCBI_TaxID=1756136;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, DOMAIN,
RP AND PATHWAY.
RC STRAIN=HF627;
RX PubMed=24848421; DOI=10.1007/s00253-014-5803-7;
RA Ishidoh K., Kinoshita H., Nihira T.;
RT "Identification of a gene cluster responsible for the biosynthesis of
RT cyclic lipopeptide verlamelin.";
RL Appl. Microbiol. Biotechnol. 98:7501-7510(2014).
CC -!- FUNCTION: Nonribosomal peptide synthetase; part of the gene cluster
CC that mediates the biosynthesis of verlamelin, a lipopeptide that
CC exhibits antifungal activity against plant pathogenic fungi
CC (PubMed:24848421). Verlamelin is a cyclic hexadepsipeptide and is
CC bridged by ester bonding between a 5-hydroxytetradecanoic acid moiety
CC and a carboxyl group on the terminal Val of amide-bonded tetradecanoyl-
CC hexapeptide D-allo-Thr-D-Ala-L-Pro-L-Gln-D-Tyr-L-Val (PubMed:24848421).
CC VlmA and vlmB are altogether regarded as essential components in the
CC biosynthesis of 5-hydroxytetradecanoic acid (PubMed:24848421). VlmA
CC catalyzes the hydroxylation at position C5 of tetradecanoic acid
CC produced in primary metabolism, while the precise function of vlmB
CC still remains to be solved (PubMed:24848421). To be loaded onto the
CC waiting NRPS, 5-hydroxytetradecanoic acid is activated in the form of
CC acyladenylate by the AMP-dependent ligase vlmC (PubMed:24848421). VlmS
CC seems to accept the fatty-acyl intermediate onto the initial module to
CC further elongate amino acid residues by the downstream modules
CC (PubMed:24848421). In addition, in the last module at its C-terminus,
CC vlmS contains a surplus condensation (C) domain that may be involved in
CC cyclization, the last step to form verlamelin (PubMed:24848421).
CC {ECO:0000269|PubMed:24848421}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000269|PubMed:24848421}.
CC -!- DOMAIN: NRP synthetases are composed of discrete domains (adenylation
CC (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation
CC (C) domains) which when grouped together are referred to as a single
CC module (PubMed:24848421). Each module is responsible for the
CC recognition (via the A domain) and incorporation of a single amino acid
CC into the growing peptide product. Thus, an NRP synthetase is generally
CC composed of one or more modules and can terminate in a thioesterase
CC domain (TE) that releases the newly synthesized peptide from the
CC enzyme. Occasionally, epimerase (E) domains (responsible for l- to d-
CC amino acid conversion) are present within the NRP synthetase. VlmS has
CC the following 7 module architecture: T-E-C-A-T-E-C-A-T-E-C-A-T-C-A-T-C-
CC A-T-E-C-A-T-C (PubMed:24848421). The epimerase domain in the first
CC module is probably non-functional (PubMed:24848421).
CC {ECO:0000269|PubMed:24848421}.
CC -!- DISRUPTION PHENOTYPE: Leads to complete loss of verlamelin production
CC (PubMed:24848421). {ECO:0000269|PubMed:24848421}.
CC -!- SIMILARITY: Belongs to the NRP synthetase family. {ECO:0000305}.
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DR EMBL; AB862312; BAO73252.1; -; Genomic_DNA.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR Gene3D; 1.10.1200.10; -; 7.
DR Gene3D; 3.30.300.30; -; 6.
DR Gene3D; 3.30.559.10; -; 11.
DR Gene3D; 3.40.50.12780; -; 4.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR Pfam; PF00501; AMP-binding; 6.
DR Pfam; PF00668; Condensation; 10.
DR Pfam; PF00550; PP-binding; 7.
DR SMART; SM00823; PKS_PP; 6.
DR SUPFAM; SSF47336; SSF47336; 7.
DR TIGRFAMs; TIGR01733; AA-adenyl-dom; 6.
DR PROSITE; PS00455; AMP_BINDING; 6.
DR PROSITE; PS50075; CARRIER; 7.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 3: Inferred from homology;
KW Isomerase; Ligase; Phosphopantetheine; Phosphoprotein; Repeat; Virulence.
FT CHAIN 1..8903
FT /note="Nonribosomal peptide synthetase vlms"
FT /id="PRO_0000438579"
FT DOMAIN 11..84
FT /note="Carrier 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 1524..1600
FT /note="Carrier 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 3084..3160
FT /note="Carrier 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 4649..4725
FT /note="Carrier 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 5753..5829
FT /note="Carrier 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 6836..6912
FT /note="Carrier 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT DOMAIN 8368..8444
FT /note="Carrier 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 13..81
FT /note="Thiolation (T) domain 1"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 59..736
FT /note="Adenylation (A) domain 7"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 572..953
FT /note="Condensation (C) domain 1"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 989..1386
FT /note="Adenylation (A) domain 1"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 1529..1597
FT /note="Thiolation (T) domain 2"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 1613..2050
FT /note="Epimerase (E) domain 1"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 2091..2523
FT /note="Condensation (C) domain 2"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 2546..2943
FT /note="Adenylation (A) domain 2"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 3089..3157
FT /note="Thiolation (T) domain 3"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 3174..3614
FT /note="Epimerase (E) domain 2"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 3655..4093
FT /note="Condensation (C) domain 3"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 4114..4512
FT /note="Adenylation (A) domain 3"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 4654..4722
FT /note="Thiolation (T) domain 4"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 4775..5191
FT /note="Condensation (C) domain 4"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 5216..5614
FT /note="Adenylation (A) domain 4"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 5758..5826
FT /note="Thiolation (T) domain 5"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 5875
FT /note="Condensation (C) domain 5"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 6311..6702
FT /note="Adenylation (A) domain 5"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 6841..6909
FT /note="Thiolation (T) domain 6"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 6923..7349
FT /note="Epimerase (E) domain 3"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 7391..7823
FT /note="Condensation (C) domain 6"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 7844..8240
FT /note="Adenylation (A) domain 6"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 8369..8441
FT /note="Thiolation (T) domain 7"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT REGION 8482..8897
FT /note="Condensation (C) domain 7"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:24848421"
FT MOD_RES 45
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 1561
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 3121
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 4686
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 5790
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 6873
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 8405
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 8903 AA; 977655 MW; 6142E7C18D693286 CRC64;
MDAPDIQAPS GSCRTTLGKV AADIFEMNVE TLDWDMSFIQ MGGDSILAID FIVRCRDEGI
WVDMMDLLTV DTLAELADSI DEQNGVTADV ANSSDLNEHE THQENGENIN ATLPVADRPL
RFASMEIAHV KDASLVSSAL ESLITRHSAL RSVWSVSSTG EYTLTTKPTA MAYESQPFFL
AEASEPTKMN DAFELLKNAL RSDGAPPLGC LFISNNATTA SSIIVLAADA NLVDSLSMRI
LRTEFREFIL GHALDAPPGF QFSNWVAAKC QSTTARPRTL QQPQRAMERA IATKLSSSTS
SADSSSNEAT ITTFQITHST TKKLFAAQTH AALRTIPAEI INAALVYVLG AHYKKNVDHL
MIKTAYSVRE QQNLPLDAVG CYEAEIEWEA PALSSHESAV FAVRRVCDAF ATPSRNSYDT
STETLYIDCT RLQDTEDDEL SSFSDTILGH GHHVSVATVA GQVHVSMQLG NEKISRESIT
NEFKLCLEKM LDELAQSPEM ATLRDYPLIH WSYSDLDDLV ADLKSQIVTI KGIESIGPSS
AVQESFFISQ AINPDSYINH VKVRMASADD SVPHQLDTEK LVYAWGNIVK RHAVLRTAFV
ESRDRPGKYD QLVFNPIAVL PRVTVFSCTP EASNTPSFQT GKFEVPMRLC VYEISTSELQ
LELDISHALV DGHSAKILLH DLRASYLQDT YFSELAPLPY TDFAFHQQTV LDAGETSDGV
AYWTSYMNKA GESHLPLITT NPNLKNLETA HRTISLPAGK LRAICGQLSI TPANLFHIAW
ALALRRIILT DTITFSYIVS GRNGSLENSE ATVGPFINTL PFSLALAPET SVTEVLDLSK
RDWQEGASFH NVPISELAVS KTRSLKRLGN TLLSIEREGS SSHPFADGSD LSLSARTSAT
DFDLTANIRF DEERIEFSVE YWASRIAWPV AKAQMSAFED AVSFLLDGVN MSIRDFPTHG
IQDKMAFLEW NTAPARLESC VHDLVLEKMA AQPTALAISA WDGEMTYGQL DHASYRVACE
LVEFGITPDT MVGMCMEKSK LGVVAMLGIL RAGGAVVPLG VQHPIARIKG IVADAQIPLI
LVDEAHKERL AELEPAAKLF AVDSFVKNDK SSPSTASSPK PCTSVGPDHV AWVIYTSGST
GAPKGVMLEH GALSTSILYH GRRLDIQSYD RLLQFAAFTF DAAIQEIITA FAFGASTCIP
SEQERMDQLP AFISREKITI TTLTSTVAAL LHPQDVPTVR TMILMGEAVQ AKVVDQWIDH
ATVINAYGPS ECCIHSTCRP VQSSLTALNI GTAIAGATWI ANPKNVGQLV PLGAPGELLL
EGPLLARGYL NDPIKTAKAF VADPAFVAEL NLSPGRRFYR TGDLAQQNPD GTITYLGRID
TQIKIRGQRV EIGEIEYHIG KQSGVHDAAV LYIREGPLAD RLVAAVNLGE STPNADQFQG
SAIQCVTGDE KDKATLQLRE IQYALSQQVM HYMVPSVWIP LYAMPMNNSG KTDRRALTLW
VQALSQSEID EITAAEADDD IDESSMSTVE QELRQIWSKV LDVPLRSVTY SANFFSLGGD
SITAMQVVSA CRARGIIVTV RKVLDCQTIP QLALAAESQA TSANEEVVTE APFPLSPIQK
MYFETVAADG LRADGETSFN QGVLLHVTRR VELAELTEAL NKTVAKHAML RARFFRTQNQ
EFQQKIERDV TGSYRLRAHA DVANAESLHG IVAESQATLD LERGPIFAAD LIERQDRQVL
HLVAHHLVID LVSWRILVQD LEEVIINKAL PNPRSMAFPT WIERQHNYLD KLMDRKTEVL
PVTVPATSWS YWGLVPGEEV YANRTSYQVK CDSNVVDLLS GHANAALKTE PVEVLLAALV
FSFQQAFPDR DVPAIFTEGH GRETIDAVSD PSDTIGWFTT MAPVYLPQRA SENAIIEVVK
QVKDQRRRIP GRGMPYFGSR FSTTRCKSQF ASHSPAEIIF NYAGRFQQLE REDALFRFDS
EDDMNTTSKI GGRVKLLSAL DVAATVEGNE LLITVNFSNQ SQHQDSIRSW VDAYGKCIES
TVKELAAVSA PIATATDFPL AHLSDADMKT IETDSDYLAV IGCSSTAELD DILPCSPIQQ
GILLTQLQSP STYCIHQTCR IRSTTTSPMD IERLIAAWKE IVSRHSILRT VLLEPLPGQE
QFMQMVLKEP RIGIKRVNDI SDDIAAEWLE SQPTLDLSEL TRPPHLLTLL TTAKGEVYCR
FDISHALVDA SSVSLIMQDL LSAYDGNLGP NVGSDYSSYV AYLEEQDSQD ELEYWTSVLR
NAEPCILSPQ DPIHDNRADS TIKRVVAHID DLSPLYKFRD TYGVSLASIC QLSWALVLAM
RTNSENISFG NLSSGRDVPI QNVQALVGPM INMLICRLSL DWDANASDVA RNLQRQVSES
FEHQRSSLAS IQHALGLSRN QPLFNSTLSY KRADSDAAAS PATGIYLEGL AWDDPTEYDL
HTNIETSKTG MEIHMQYSTA VFSDNAATKM IEGLTRAIQA VCVGGETPLS QLQLLSVSEE
NKLRQWNAVA TPRLERCVHE LVLEKMSSHA DATAISAWDG SMTYQELNNA SIQLAHHLVA
QGVRPEVKIG LCLDKSRLGV VAMLATLRAG GAVVPLGVQS PVARIETIVN DSEMKIVLVD
RNNQERLNTL ASTVQLLAVD QFAQTMSTPT DILLKEPCSS VQPDNTAWII YTSGSTGIPK
GVVLEHGSIA TSMRAHGPAI GIQPQDRVSQ FAAYTFDVSI AETMTTLAYG ACICIPSEDD
RINRLTGFLS EHKVTIATLT STVASLVQSI DTPTIKTLVL TGEAVQPNVV DQWKQHNTTV
INAYGPSESS IWATSKIVED SKDALNIGLP LSGAFWVVNR NNIGQLVPVG SPGELLIEGP
LLARGYLNDQ IKTAASFVVD PAFIHDLGFT TGRRMYRTGD LVQQNDDGTM VYLGRQDSQV
KIRGQRVEIG EIEYHVGKQE GVQDAAVLHM KDGPLADRLV AIVIPRNDDL KTTRGQNDAQ
ITQIPQQFKE DTKRHLQGVK QKLSQLVMQY MVPNVWIPLV AMPVNMSGKM DRLALNRWIQ
SLSKDELAFM TGTEETQDPD QDKLFTTAIE RQLRQVWSDV LGVSVHAVTY TSNFFSLGGD
SITAMQVVSM SRSHGILVTV RTVLECQTIP ELALQAKMVD GDNSQLTRVP EGPFALSPIQ
QMYFANISGD GIRADGNYRF NQAVSLYIST HISQEQLKHA LDAVVSKHAM LRARYSQGPA
GWQQWIEKDV SGGFRCQSYD AVDLDAMRQI IETSQTSLDI EHGPVFAADL IERQDNDRQV
LHLVAHHLSI DLVSWRIVIQ DLEQLLTNGK LPNPTTLSFP VWLERQQDSL DTFIAKMDTP
ESALSQLLPT SVPVIDWNYW GLSPGQEVYG SLTSLETRCD SATTSLLLDQ ANSALKTEPV
EILLAALLSS FQQVFTDRQS PAVFTEGHGR EAFDTKSELD LSETVGWFTT MTPVYIPQSA
ATNSIQMLQK IKDQRRRVPG RGMPYFGSRY LTSAGEEKFA DHATPEILFN YFGRFQQFER
DDALFQINND DDSASSQFGD LIKLFAALDV TVAVEASELH IKTRFSRQSQ HQASIQRWVQ
AYGNAIKSLV EELMVTAATS TATDFPLARL TDTNWEFMQK QYLVAMNLQS TAEIEDILPC
SPMQQGILLT QLQSPTTYCI HQISRFQPSK SGSVSVERLI SSWKHVVSRH SILRTILVEP
LPGQERFVQI VLKQPHLDII KLREITDSEH AAIATLEAQP LLNARKITSP PHRITMLQST
AGEVYCRFDI SHALIDGSSM AILIRDLMSA YSEDVSSDSI ASGSKYSSYV AYLEDTDHQK
ADLQYWTSLL ADSEPCILPS EASAPESEPA QLGHVSKTIS DLDALHGFRD THNVSIASIC
QLAWALVLST WTGSSDISFG NLSSGRDVPI EGVQDLVGPM INMLICHVPL DWNASVADVA
RKIQSQSAEA FEHQRSSLAA IQHELGLSRD KPLFNTTLSY KRITPPPSSS GSTSITFEAL
VQEDPTEHDL HVNIDSTPTG LQFDIQFSTA VFSSAAAETL TESLVRTVGI LSQSAHLSLG
NVNLMSTKDI QQLCEWNSKM PSRTELCVHD LISERLNTQP ESMAISAWDG DMSYLELDAV
SHTLASHLIT LGLDFQRSEP MIGLCMDKSK WAVVAMLAIL RAGGTVVPLG VQHPVSRIDN
IVQDTSAIVV IVDRGQEQRL ASLGTSTHLL AIESFFEASP PVASQSTPLT ADTTPDSAAW
VIYTSGSTGK PKGVVLEHGA LVTNILAHGR AMNIQPGDRV LQFAAYTFDI SIAEVLTTLI
FGACVCVPSE SERMEQLACF ISRQEVTTAI LTSTVAALVD PQQTPTLQTL VLTGEAVQPK
VVSQWIGQAT VMNCYGPSES WICTTHKIES AATASVVGPP IAGGFWVVNP GSVDQLVPIG
APGELLIEGP LLAREYLNNA DKTAASFIND LAFTKELGLG SRRMYRTGDL VKYNSNGALV
YIGRIDTQIK IRGQRVEIGE IENQIVDLLP GAREAVVDLI TPAEVEGASP MLVAVVEYRQ
DEPRTDATGL SLYDPSQLTD ATLEALDQLQ TDIAKALPAY MIPATFLLAS KLPINASGKL
DRRTLRLALQ DMTREELGNS TGNTSTKQAP RTTMEKKLRD LFAATLQLTP DNFGINDSFF
RLGGDSVAAM KMTAAARALD LPLSVVDIFR FPILADLAEA TELKCSQQQE DSRSLVPFSL
WPELQQDHAS SSDSYKTQLL AEAAQLCGVS ASQIEDVYPC SPLQAGLMAI TSQRPEAYIM
RRAFKLRASF PIEQLKIAYE RLTEAVSILR TRIIPSTCVD ALQVVVQEKP FWHGEVGMSL
EQYIAKDRSA SMAYGRALSR TAIVSNEDGQ FFVWTMHHSV YDGWSLTKMM GMLTQLMTGQ
ALATTVPSSR FINYLVQQDT DQVAKFWQSH FQGANWTRFP ALPSPRYQAK SSGQLRSQFQ
LPLNPSILET DSTVLRAAWA LLVASKIGAD EAVINVVLSG RMAPVEDIMN MLTPTVTTVP
VRVSATKNQS INKFLKTIHD TAIDMVPFEH TGLQNIRTMV PTLGSDFDPG HTFVVQPAGE
SESAATMWNM DLEREATPLD AFDAYALTVE CTVDSQRTGE VTVDIRYDSF VIPDDDAQKL
LNQFTHIAQE LAQQAATTKP LAQLQMLSEE DRFLLSKWNA HVPPRLEYTL HDAVTETMTS
QPDAPAIYSW DGDMTYGEVN AASHRLASHL ANQGVGPEVM VGLCMDKSKW AIVSMLAILR
AGGAVVPLGI SHPLARIDNI IQDTAAPLVL VDSTHQHRLQ DLTAPTPLLA VDKFFEEYEA
ANYDSSAKLP STVQPHHPAW VIYTSGSTGT PKGIVLEHRA LATSILSHGK EFGIQAHDRV
LQFAAYTFDV AIQDVIATLA SGACLCVLSE YDRINRLTEF LSESNVSFAI LTPTVAALIE
PKDVPTVKTL VLGGEALPAK VVDQWAEHAL IINGYGPSEC CIHSTCAKIP LGSDARNIGR
GVTANTWVVD PTDIGQLVPI GSPGELLIEG PLLARGYLND PTKTAKSFIR DPAFLSTLNL
PNGRRMYRTG DLVQQNRDGS LIYIGRRDTQ VKIRGQRVEI GEIESRIVDL LPEAREAVVN
LVRPAGEAAD LVTLVAVIEC DYAAGASHDT ESELELFKPS SYSDALNRAL VKLDDDLGQA
LPSYMVPSAY LLVPKLPLNP SGKLDRRAIQ DQLQLLPRAK INSLSGLTNR KQAPTTAMEK
RLQNLFCQTL MLTPEEVGVN DSFFRIGGDS IAAMKLTAVA RHQNLPVSAA DIFRWPRLGD
LAQELEQRHE LKTATLNDPA PLSLWPELSQ AGTQSKSQLL ANIASQCGVS VEAIEDVYPC
SALQAGLMAI TTQRPEAYVV QRVFKLQPSL SSQHFKAAWN QLAQSLPILR TRIVPSIHTD
ALQVVTRDAP VWQETQASVQ DYLENDRTVP IAYGTPLSRV AIVQDQQSRY FVWTIHHSAY
DGWSMGKMME VLSQVLEGTT PSVLVPVSRF IGYLSQQDKS QTSTFWQKHF EGASCTVFPE
LPSRQHVVNP NKTLKSRIQI SQSPGVTPFT ALRAAWALVV ASATGSDDAL INVVLSGRLA
SVDGIMDLVA PTITTVPFHV PISQDLSVKE FLANVDERAS DMIPYEHTGL QHIRRMVPGL
GPEFSPGHVF VVQPAAESES TVAALPQMEL IKSDFESEDA FHAQALTVEC TVGQDLSDVE
VQMRYDGNVL STESATHLLD QFSHVVEQLA LNGDKSLSQL ELLTANDRQR LIEWNSTVPP
RVERCIHQLV EEQMSLRPSE LAIKAWDGDM TYAELDTSSR QLAQRLTQMG VGPDVMVGIC
MDKSKMGVVA ILAILRAGGA VVPLGVTHPL TRIEGIVKDT KSPLILVDSA QKQRLASLTA
QLLVVDSTLT NTLTASAQNI SVQSKNVAWV VYTSGSTGTP KGVVLEHGAL ATSVLGHGAA
YNVRSDDRIL QFAAYTFDAA IQEIITTLAF GACICVPSEQ DRVNRLTDFF IETGITMATL
TSTVAGLVRP NMTPAVRTII LVGEAVQANV VDQWIQKATV INGYGPSECS IASTCGEIRH
SSYALNIGTA IAGATWIVGS TNKLVPIGTP GELLLEGPLL ARGYLNDAVK TAASFTTNAA
FVEELGLSSA NRRMYRTGDL VKQNIDGSIT YLGRMDGQIK IRGQRVEIGE IEHHLQKHSV
VGDAVVLYMK QGPLSGRLIA IVVTNDTNST SQTAEIQHLP TGQRESANLE LTDVQQSLSN
QLMQYMIPSV WIALASIPVN ISGKTDRLTL TRWLQSLSDD EVEALTGTEE TEVDESSATN
TERQLRQIWS QVLDVPIEKI TFSSTFFSLG GDSITAMQVV SACRSCGLLV SVQKVLNCQT
IPELAATLEV MDIVNDVDQI PEGFFELSPI QRMYFDDMAA MGLRADGENR FNQAVTLRIT
RPTTSEELIQ ATDILVAKHP MLRARFIQNQ QSWQQHIEKE VAGSYRFELH EVADAQAMQS
IITQSQASLN LEHGPVFAID LIDLPTKKVL HLVAHHLVID LVSWRVLAQN LEDLLTSGTE
PNPTSLSFPS WIQKQFQFLP SSEETSESVL PVQIPASNWE YWGLVPGSER FGNRSKIEVK
CDTSTTSLLT GDANYAFNTE PVEVLLAALA ISFQKTFTDR SMPAVFVEGH GRETMDDKVD
LSDTVGWFTT MTPVHVPMDK NESDLDVLRR TKDQRRRIPG RGLPYFASRF LGPNPDKFDN
HGPAEILFNY FGRFQQLERE NSVFQIEQDG ESAPQLGNSV KLFAALDLSI AIEGDQLSIT
VHYSNKSKHQ STIRQWAESY GRTIKTLVEA LVIAPPTSTA TDFALARLSD SDMVAIEKDC
VSNVGSTRNI EDILPCSPIQ QGILLSQLQS PTTYSIYQTC RIKPSKHDSL VDAHRFLGAW
KQLVAHHSIL RTVLLEPLPG HEKFMQIVLR EPEINVLTKS GVADAEAVEW INSRPGLDLT
DRHHPPHRLT LLTTTSGQVY CRFDISHALV DASSLALIIR DLMSAYEGKL GSSSNGSNYS
AYIAYLDDNN QQDDLNYWTS LLNNAEPCLV PPKEPTHTAT QATIGHASQK VSDLDVLHKF
RDTYGISIAS ICQLSWALVL ATWTGSQNVS FGNLSSGRDA PIPGAQDLVG PMINMLVCHL
QLDWDSKVSD AARKIQTQSS EAFEHQRVSL ASIHHALGLS KDQPLFNSVM SYKRLATGES
TPREIILEGL TAEDPSEYDV NVHINASSTS LDFNIQFSTT VLSQAAANKL TASLVQAVHA
ITQNANRSLG QLKLVLTNDE AQICKWNSFM PAGLQNRVHD HVLEQMARKP EAQAIFASDG
QMSYGELDVS SRQLAHHLVS QGVGPDVVVG VCMDKSRWAV VAMLSILRAG GAVAPLGVQH
PVARIDTIVK DASAPVILVD AEQEQRLDTL SNNFQLINVK SFFDTVQNTV STSEPCTTVQ
SHHIAWVLYT SGSTGVPKGV MLEHGSLATS IMLHSRRFAM QSTERLLQFA AFTFDAAVFD
IFAPLSHGGC TCIPSEHDRM NNLEAFAIGA KVTWGFFTPT VAALMQPSDI PSMRTLILGG
EVVTAKGVDH WVKAGVKVIN VYGPTECSIY STYKHIQDTQ NLRNIGTTVA AGLWVVNPVS
GEQLVPIGAP GELLIEGPLL ARGYLNDSAK TAASFVTDPK FVKELGLSPG RRMYRTGDLV
QQNSDGTLTY LDRIGTQVKI HGQRLEIGEI ESQLHDLLPE SRYVCVCKKG TSLVAVIEST
TPNRDTPGTS AHYIVAPGPE QEKTFEYLNS TLREKLPSYM IPSAFLVINE FPLNDNGKFD
RRRIGNLLNS IPSDKWLEYT AKSQTYYAPI SATEAVFCEL WSQCIQQLDK PVSRTDNFFD
LGGDSVTAMQ LVQQLAKRGM RLATIDIFNN PVLHAMAACV SDVSDDNQEY KRFSLISTEE
KSTALELVAA DDTVDKQIRV IDVLPTTEFQ TLMVRQIMSA ARRQLNQFAF DADEACDVSV
LTSAISDLVA TIESLRAGFV KLPGQKYLQV VYAVWEPEIR VFYTEKSPRA FYEESSEQDL
FPEPTLSRPL FDVAIIIDKI TQKHRVVFRI SHALYDGATL HRVWTALEAL TTGQAPGYFA
PIGAYLQSLQ AQTTSETEDY WQQLVDGATI SCVGTSSEPK VSRLGHVSGP PITLPESKQS
HFNLAVAVKA AWALVFGHHA NTHDIVFADV MTGRNTVDSS VADVVSCCAR AVPCRITYEP
DWTVEKLLDL TKQQQVNSMR HEGLELQQIA QRYMGWPQDD HEEAPDMRVS MTNYVKTSIR
DLLLGATQYR RATAGFQNAY ASADFSVDSV EESDGSLSVS IAYAADRISE QLAATLLHRT
RVTLEKMMEN PRSTVGHLLK QLD
