VLN4_ORYSJ
ID VLN4_ORYSJ Reviewed; 946 AA.
AC Q0JAD9; Q7XTQ0;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Villin-4 {ECO:0000303|PubMed:20807878};
GN Name=VLN4 {ECO:0000303|PubMed:20807878};
GN OrderedLocusNames=LOC_Os04g51440 {ECO:0000305},
GN Os04g0604000 {ECO:0000312|EMBL:BAF15698.1};
GN ORFNames=OsJ_16056 {ECO:0000312|EMBL:EEE61628.1},
GN OSJNBa0041A02.24 {ECO:0000312|EMBL:CAD41877.2},
GN OSNPB_040604000 {ECO:0000312|EMBL:BAS90870.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=20807878; DOI=10.1105/tpc.110.076240;
RA Khurana P., Henty J.L., Huang S., Staiger A.M., Blanchoin L., Staiger C.J.;
RT "Arabidopsis VILLIN1 and VILLIN3 have overlapping and distinct activities
RT in actin bundle formation and turnover.";
RL Plant Cell 22:2727-2748(2010).
CC -!- FUNCTION: Ca(2+)-regulated actin-binding protein (By similarity). Binds
CC actin microfilaments (MFs). Involved in actin filament bundling,
CC severing and capping. Caps the barbed end of actin filaments and is
CC able to sever them in a calcium-dependent manner (By similarity).
CC {ECO:0000250|UniProtKB:O81644, ECO:0000250|UniProtKB:Q10L71}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:O81644}.
CC -!- SIMILARITY: Belongs to the villin/gelsolin family. {ECO:0000305}.
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DR EMBL; AL606638; CAD41877.2; -; Genomic_DNA.
DR EMBL; AP008210; BAF15698.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS90870.1; -; Genomic_DNA.
DR EMBL; CM000141; EEE61628.1; -; Genomic_DNA.
DR RefSeq; XP_015635692.1; XM_015780206.1.
DR AlphaFoldDB; Q0JAD9; -.
DR SMR; Q0JAD9; -.
DR STRING; 4530.OS04T0604000-02; -.
DR PaxDb; Q0JAD9; -.
DR PRIDE; Q0JAD9; -.
DR EnsemblPlants; Os04t0604000-01; Os04t0604000-01; Os04g0604000.
DR EnsemblPlants; Os04t0604000-02; Os04t0604000-02; Os04g0604000.
DR GeneID; 4336902; -.
DR Gramene; Os04t0604000-01; Os04t0604000-01; Os04g0604000.
DR Gramene; Os04t0604000-02; Os04t0604000-02; Os04g0604000.
DR KEGG; osa:4336902; -.
DR eggNOG; KOG0443; Eukaryota.
DR HOGENOM; CLU_002568_2_1_1; -.
DR InParanoid; Q0JAD9; -.
DR OMA; HIWITEN; -.
DR OrthoDB; 1376537at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR GO; GO:0032432; C:actin filament bundle; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0051015; F:actin filament binding; ISS:UniProtKB.
DR GO; GO:0051693; P:actin filament capping; ISS:UniProtKB.
DR GO; GO:0007015; P:actin filament organization; ISS:UniProtKB.
DR GO; GO:0051014; P:actin filament severing; ISS:UniProtKB.
DR Gene3D; 1.10.950.10; -; 1.
DR Gene3D; 3.40.20.10; -; 6.
DR InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR InterPro; IPR007123; Gelsolin-like_dom.
DR InterPro; IPR036180; Gelsolin-like_dom_sf.
DR InterPro; IPR007122; Villin/Gelsolin.
DR InterPro; IPR003128; Villin_headpiece.
DR InterPro; IPR036886; Villin_headpiece_dom_sf.
DR PANTHER; PTHR11977; PTHR11977; 1.
DR Pfam; PF00626; Gelsolin; 4.
DR Pfam; PF02209; VHP; 1.
DR PRINTS; PR00597; GELSOLIN.
DR SMART; SM00262; GEL; 6.
DR SMART; SM00153; VHP; 1.
DR SUPFAM; SSF47050; SSF47050; 1.
DR SUPFAM; SSF82754; SSF82754; 2.
DR PROSITE; PS51089; HP; 1.
PE 3: Inferred from homology;
KW Actin capping; Actin-binding; Calcium; Cytoplasm; Cytoskeleton;
KW Reference proteome; Repeat.
FT CHAIN 1..946
FT /note="Villin-4"
FT /id="PRO_0000438167"
FT REPEAT 28..109
FT /note="Gelsolin-like 1"
FT /evidence="ECO:0000255"
FT REPEAT 152..219
FT /note="Gelsolin-like 2"
FT /evidence="ECO:0000255"
FT REPEAT 274..339
FT /note="Gelsolin-like 3"
FT /evidence="ECO:0000255"
FT REPEAT 641..715
FT /note="Gelsolin-like 4"
FT /evidence="ECO:0000255"
FT DOMAIN 881..946
FT /note="HP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00595"
FT REGION 744..789
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 801..832
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 844..902
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 773..787
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 635..637
FT /note="ENL -> GNQ (in Ref. 1; CAD41877)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 946 AA; 105657 MW; 874D1348A799DE89 CRC64;
MSISMKDVDP AFRGVGQKDG LEVWRIENFK PVPVPTSSHG KFYMGDSYII LKTTALKNGS
FRHDLHYWLG KDTSQDEAGT AAILTVELDA ALGGRAVQYR EVQGGETEKL LSYFRPCIMP
QPGGVASGFN HVEVNQQDHV TRLYVCQGKH VVHVKEVPFV RSSLNHEDIF ILDTANKIFQ
FNGSNSCIQE RAKALEVVQY IKDTFHEGKC EVAAVEDGKL MADTEAGEFW GLFGGFAPLP
KKTSSEDNGD DKETVTKLLC FNQGTLEHIS FESLEHELLE TNKCYLLDCG AEMYVWMGRG
TSLQVRKGAS EAAEKLLIDE NRKGSNVIKV IEGFETIMFK SKFNKWPPTP DLKLSSEDGR
GKVAALLRSQ GLDVKGLMKA APEEEEPQPY IDCTGHLQVW RVNGDGKTLL SSSDQSKLYT
GDCYIFQYTY TGDDKEECLI GTWFGKKSVE EDRTSAISLA SKMFQAAKFQ AAQARLYEGK
EPIQFFVIFQ SLQVFKGGLS SGYKNFIAVN GTDDDTYVEG GLALFRIQGS GSENMQAIQV
DAVSSSLNSS YCYILHNGNT VFTWTGNLTT SLDNDLVERQ LDVIKPDLPS RSQKEGRETD
QFWELLGGKC KYSNKKIGKE NESDPHLFSC ILSKENLKVK EIHHFTQDDL MAEDIFVLDC
RTDLFVWVGQ EVDAKLRSQA MDIGEKFLLH DFLMENLSQD TPIFIVTEGS EPQFFTRFFT
WDSAKSLMHG SSYQRKLAIV KGGATPSLDK PKRRTPAFSG RNAGQDKSQQ RTRSMSHSPE
RHRIRGRSPA FTAIASAFEN PSTRYLSTPP PAVKKLFPRS GGSELPKTSS KQSAINALTS
AFEGPTKSTI PKSVKASPEA EKAIQEEGST IGESENEPED DENSTIYPYE RLTTTSDDPA
PDIDVTKREV YLSSVEFTEK FGMTRASFKN LPKWKQNRLK SDLQLF
