ID   VLP21_BORHE             Reviewed;         364 AA.
AC   P21875;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Variable large protein 21 {ECO:0000303|PubMed:9453591};
DE   AltName: Full=Variable major outer membrane lipoprotein 21 {ECO:0000303|PubMed:1706456};
DE   Flags: Precursor;
GN   Name=vlp21 {ECO:0000303|PubMed:9453591};
GN   Synonyms=vmp21 {ECO:0000303|PubMed:1706456};
OS   Borrelia hermsii.
OG   Plasmid bp21E, and Plasmid lp7E.
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borrelia.
OX   NCBI_TaxID=140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35209 / HS1; PLASMID=lp7E;
RX   PubMed=1706456; DOI=10.1111/j.1365-2958.1990.tb00549.x;
RA   Burman N., Bergstroem S., Restrepo B.I., Barbour A.G.;
RT   "The variable antigens Vmp7 and Vmp21 of the relapsing fever bacterium
RT   Borrelia hermsii are structurally analogous to the VSG proteins of the
RT   African trypanosome.";
RL   Mol. Microbiol. 4:1715-1726(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35209 / HS1; PLASMID=bp21E;
RX   PubMed=1484486; DOI=10.1111/j.1365-2958.1992.tb02198.x;
RA   Restrepo B.I., Kitten T., Carter C.J., Infante D., Barbour A.G.;
RT   "Subtelomeric expression regions of Borrelia hermsii linear plasmids are
RT   highly polymorphic.";
RL   Mol. Microbiol. 6:3299-3311(1992).
RN   [3]
RP   PROTEIN SEQUENCE OF 55-75; 186-208 AND 245-259, AND SUBCELLULAR LOCATION.
RX   PubMed=2409197; DOI=10.1084/jem.161.6.1302;
RA   Barstad P.A., Coligan J.E., Raum M.G., Barbour A.G.;
RT   "Variable major proteins of Borrelia hermsii. Epitope mapping and partial
RT   sequence analysis of CNBr peptides.";
RL   J. Exp. Med. 161:1302-1314(1985).
RN   [4]
RP   FUNCTION.
RC   STRAIN=ATCC 35209 / HS1; PLASMID=bp21E;
RX   PubMed=2385585; DOI=10.1073/pnas.87.16.6077;
RA   Kitten T., Barbour A.G.;
RT   "Juxtaposition of expressed variable antigen genes with a conserved
RT   telomere in the bacterium Borrelia hermsii.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:6077-6081(1990).
RN   [5]
RP   NOMENCLATURE.
RX   PubMed=9453591; DOI=10.1128/iai.66.2.432-440.1998;
RA   Hinnebusch B.J., Barbour A.G., Restrepo B.I., Schwan T.G.;
RT   "Population structure of the relapsing fever spirochete Borrelia hermsii as
RT   indicated by polymorphism of two multigene families that encode immunogenic
RT   outer surface lipoproteins.";
RL   Infect. Immun. 66:432-440(1998).
CC   -!- FUNCTION: The Vlp and Vsp proteins are antigenically distinct proteins,
CC       only one vlp or vsp gene is transcriptionally active at any one time.
CC       Switching between these genes is a mechanism of host immune response
CC       evasion. {ECO:0000269|PubMed:2385585}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305|PubMed:2409197};
CC       Lipid-anchor.
CC   -!- MISCELLANEOUS: Genes for both Vlp and Vsp families are on (usually)
CC       unnamed linear plasmids in B.hermsii HS1. {ECO:0000305|PubMed:9453591}.
CC   -!- SIMILARITY: Belongs to the variable large protein (Vlp) family. Alpha
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M57256; AAB59031.1; -; Genomic_DNA.
DR   PIR; S11981; S11981.
DR   AlphaFoldDB; P21875; -.
DR   SMR; P21875; -.
DR   PRIDE; P21875; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000680; Borrelia_lipo.
DR   Pfam; PF00921; Lipoprotein_2; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Direct protein sequencing; Lipoprotein; Membrane;
KW   Palmitate; Plasmid; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000305"
FT   CHAIN           27..364
FT                   /note="Variable large protein 21"
FT                   /id="PRO_0000018089"
FT   LIPID           27
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305"
FT   LIPID           27
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   364 AA;  37084 MW;  16598B639EE63776 CRC64;
     MRKRISAIIN KLNISIMMMI VVLMIGCGQQ PEAGKTGAAG GEKQGAGSLS EVLMEVGKSA
     ENAFYSFLEL VSDTLGFTAK STTKKEDVGG YFNSLGGKLG EASNELEQVA KNSEAGIEKN
     DASKNPIRSA VNAAKKTLEA LKGYLDSLGT VGDSNPVGWA SNNAQGAAVD EAELKKAYKA
     LKGIMDTAEG AGVARPEVGN IAVKVGNGTD NKDGAKILAT DGAAAVGDAG KAAAILTTVS
     GKEMLASIVN STEDKAVKIT GNVTVETTPL EFAVGGNGAH LSQNANSKAA AVAGGIALRS
     LVKGGKLAAD NNDDDKASQG VGITAANKLL VAVEDIIKKT VKNVLEKAKG EIDKARDPKP
     AGQQ