ID   VLP25_BORHE             Reviewed;         362 AA.
AC   P32779;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Variable large protein 25 {ECO:0000303|PubMed:9453591};
DE   AltName: Full=Variable major outer membrane lipoprotein 25;
DE   Flags: Precursor;
GN   Name=vlp25 {ECO:0000303|PubMed:9453591};
GN   Synonyms=vmp25 {ECO:0000303|PubMed:1484486};
OS   Borrelia hermsii.
OG   Plasmid {ECO:0000303|PubMed:1484486}.
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borrelia.
OX   NCBI_TaxID=140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35209 / HS1;
RX   PubMed=1484486; DOI=10.1111/j.1365-2958.1992.tb02198.x;
RA   Restrepo B.I., Kitten T., Carter C.J., Infante D., Barbour A.G.;
RT   "Subtelomeric expression regions of Borrelia hermsii linear plasmids are
RT   highly polymorphic.";
RL   Mol. Microbiol. 6:3299-3311(1992).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=9453591; DOI=10.1128/iai.66.2.432-440.1998;
RA   Hinnebusch B.J., Barbour A.G., Restrepo B.I., Schwan T.G.;
RT   "Population structure of the relapsing fever spirochete Borrelia hermsii as
RT   indicated by polymorphism of two multigene families that encode immunogenic
RT   outer surface lipoproteins.";
RL   Infect. Immun. 66:432-440(1998).
CC   -!- FUNCTION: The Vlp and Vsp proteins are antigenically distinct proteins,
CC       only one vlp or vsp gene is transcriptionally active at any one time.
CC       Switching between these genes is a mechanism of host immune response
CC       evasion. {ECO:0000250|UniProtKB:P21875}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000250|UniProtKB:P21875}; Lipid-anchor.
CC   -!- MISCELLANEOUS: Genes for both Vlp and Vsp families are on (usually)
CC       unnamed linear plasmids in B.hermsii HS1. {ECO:0000305|PubMed:9453591}.
CC   -!- SIMILARITY: Belongs to the variable large protein (Vlp) family. Alpha
CC       subfamily. {ECO:0000305}.
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DR   EMBL; L04787; AAA22965.1; -; Genomic_DNA.
DR   PIR; I40304; I40304.
DR   AlphaFoldDB; P32779; -.
DR   SMR; P32779; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR000680; Borrelia_lipo.
DR   Pfam; PF00921; Lipoprotein_2; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000305"
FT   CHAIN           27..362
FT                   /note="Variable large protein 25"
FT                   /id="PRO_0000018091"
FT   LIPID           27
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305"
FT   LIPID           27
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   362 AA;  36469 MW;  2A3EF5248D5637FD CRC64;
     MRKRISAIIN KLNISIMMMI VVLMIGCGQQ AVEAGKDGAA AATGGRSLSE VLMEVGKSAE
     NAFYSFMALV PDTLGLRVTK DTKKNEVGGY FNSLGGKLGK ASDELEEVAK KSEVEGAKDG
     PIAVAIRAAV DTAKTTLSTL KEHLESLKGI GDDDKVGEAT SNQNGVAAST DELKGAFKAL
     KGIVDTAGKE GVAKPKAGDT AVKIGNADNK DGAKVLAAAA NAGRAVGDKA AAIVSAVSGE
     EMLASIVASQ EGDADAALAA DATAQTSALK FARGGGNAGQ LAKEAAKAAA VAGGIALRSL
     VKGGKLAANN NDDDKVVQSA GVTAVNKLLV AVEGIIKKTV KNVLEKAKGE IDKARAPKAT
     GQ