ID   VLP4_BEAB2              Reviewed;         230 AA.
AC   J4KLP6;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Vacuole-localized protein 4 {ECO:0000303|PubMed:28557308};
DE   Flags: Precursor;
GN   Name=VLP4 {ECO:0000303|PubMed:28557308}; ORFNames=BBA_08480;
OS   Beauveria bassiana (strain ARSEF 2860) (White muscardine disease fungus)
OS   (Tritirachium shiotae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Cordycipitaceae; Beauveria.
OX   NCBI_TaxID=655819;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ARSEF 2860;
RX   PubMed=22761991; DOI=10.1038/srep00483;
RA   Xiao G., Ying S.-H., Zheng P., Wang Z.-L., Zhang S., Xie X.-Q., Shang Y.,
RA   St Leger R.J., Zhao G.-P., Wang C., Feng M.-G.;
RT   "Genomic perspectives on the evolution of fungal entomopathogenicity in
RT   Beauveria bassiana.";
RL   Sci. Rep. 2:483-483(2012).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX   PubMed=28557308; DOI=10.1111/1462-2920.13803;
RA   Chu Z.J., Sun H.H., Zhu X.G., Ying S.H., Feng M.G.;
RT   "Discovery of a new intravacuolar protein required for the autophagy,
RT   development and virulence of Beauveria bassiana.";
RL   Environ. Microbiol. 19:2806-2818(2017).
CC   -!- FUNCTION: Vacuolar protein required for aerial conidiation and conidial
CC       maturation (PubMed:28557308). Also involved in blastospore production
CC       and cell cycle (PubMed:28557308). Plays a vital role in the secretion
CC       of Pr1 proteases for cuticular penetration and hence contributes
CC       significantly to host infection and virulence (PubMed:28557308).
CC       {ECO:0000269|PubMed:28557308}.
CC   -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:28557308}.
CC       Note=Localizes in vacuoles of germinating conidia, germ tubes and
CC       hyphal cells (PubMed:28557308). {ECO:0000269|PubMed:28557308}.
CC   -!- DISRUPTION PHENOTYPE: Affects proper autophagosome formation, asexual
CC       and blastospore development, and virulence (PubMed:28557308). Leads to
CC       severe defects in secretion of cuticle-degrading Pr1 proteases and
CC       melanisation of infected insects (PubMed:28557308). Reduces the
CC       transcript levels of all autophagy-related (ATG) genes including ATG1,
CC       ATG5 and ATG8, except unaffected ATG26 (PubMed:28557308).
CC       {ECO:0000269|PubMed:28557308}.
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DR   EMBL; JH725184; EJP62569.1; -; Genomic_DNA.
DR   RefSeq; XP_008601799.1; XM_008603577.1.
DR   AlphaFoldDB; J4KLP6; -.
DR   EnsemblFungi; EJP62569; EJP62569; BBA_08480.
DR   GeneID; 19891492; -.
DR   HOGENOM; CLU_087081_0_0_1; -.
DR   InParanoid; J4KLP6; -.
DR   PHI-base; PHI:7192; -.
DR   Proteomes; UP000002762; Unassembled WGS sequence.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
PE   3: Inferred from homology;
KW   Reference proteome; Signal; Vacuole.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..230
FT                   /note="Vacuole-localized protein 4"
FT                   /id="PRO_5003779112"
SQ   SEQUENCE   230 AA;  22965 MW;  9FC28BCAE44CF5E3 CRC64;
     MRVSSAIFTI ASGIAAVSAA DCAAAGDYDS QGRYSCNPAH QYPNGQSCKT IDGCPLLADA
     SGQPIYKSTC AAPGDYDDKG RYSCNPAHQY PNGQSCKTVE GCPLLVDANG QPIVKATGTS
     TGTGTVTVTS SAAAQPTSTG TPSSKCAAPG DYDSKGRYSC NPAHQYPNGQ TCKVIDNCPL
     LCDASGKPIV KATGTGSGNN GKPTDLPIVG AGSVLTGGMA MVAAAVVAAI