ID   VLTF1_VACCW             Reviewed;         260 AA.
AC   P68613; P21029; Q76ZT9;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   02-JUN-2021, entry version 59.
DE   RecName: Full=Late transcription factor 1;
DE            Short=VLTF-1;
DE   AltName: Full=Trans-activator protein GK1;
GN   Name=VLTF1; OrderedLocusNames=VACWR086; ORFNames=G8R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=2344616; DOI=10.1016/0092-8674(90)90190-p;
RA   Keck J.G., Baldick C.J. Jr., Moss B.;
RT   "Role of DNA replication in vaccinia virus gene expression: a naked
RT   template is required for transcription of three late trans-activator
RT   genes.";
RL   Cell 61:801-809(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS OF ARG-60.
RX   PubMed=2024483; DOI=10.1016/0042-6822(91)90585-y;
RA   Meis R.J., Condit R.C.;
RT   "Genetic and molecular biological characterization of a vaccinia virus gene
RT   which renders the virus dependent on isatin-beta-thiosemicarbazone (IBT).";
RL   Virology 182:442-454(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=2476568; DOI=10.1128/jvi.63.10.4224-4233.1989;
RA   Wright C.F., Moss B.;
RT   "Identification of factors specific for transcription of the late class of
RT   vaccinia virus genes.";
RL   J. Virol. 63:4224-4233(1989).
RN   [5]
RP   INDUCTION.
RX   PubMed=2041091; DOI=10.1128/jvi.65.7.3715-3720.1991;
RA   Wright C.F., Keck J.G., Tsai M.M., Moss B.;
RT   "A transcription factor for expression of vaccinia virus late genes is
RT   encoded by an intermediate gene.";
RL   J. Virol. 65:3715-3720(1991).
RN   [6]
RP   INTERACTION WITH THE LATE TRANSCRIPTION FACTORS VLTF-2; VLTF-3 AND THE LATE
RP   TRANSCRIPTION ELONGATION FACTOR H5/VLTF-4.
RX   PubMed=15518812; DOI=10.1016/j.virol.2004.08.017;
RA   Dellis S., Strickland K.C., McCrary W.J., Patel A., Stocum E., Wright C.F.;
RT   "Protein interactions among the vaccinia virus late transcription
RT   factors.";
RL   Virology 329:328-336(2004).
CC   -!- FUNCTION: Associates with RNA polymerase to initiate transcription from
CC       late gene promoters. {ECO:0000269|PubMed:2344616,
CC       ECO:0000269|PubMed:2476568}.
CC   -!- SUBUNIT: Interacts with the late transcription factors VLTF-2 and VLTF-
CC       3. Interacts with the late transcription elongation factor H5/VLTF-4.
CC       Interacts with itself. {ECO:0000269|PubMed:15518812}.
CC   -!- INTERACTION:
CC       P68613; P07242: VACWR103; NbExp=4; IntAct=EBI-7273218, EBI-7272435;
CC       P68613; P68613: VLTF1; NbExp=2; IntAct=EBI-7273218, EBI-7273218;
CC       P68613; P07610: VLTF2; NbExp=4; IntAct=EBI-7273218, EBI-7273273;
CC       P68613; P68318: VLTF3; Xeno; NbExp=3; IntAct=EBI-7273218, EBI-7366338;
CC   -!- INDUCTION: Expressed in the intermediate phase of the viral replicative
CC       cycle. {ECO:0000269|PubMed:2041091}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae VLTF-1 family.
CC       {ECO:0000305}.
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DR   EMBL; M34067; AAA48347.1; -; Genomic_DNA.
DR   EMBL; J03399; AAB59819.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89365.1; -; Genomic_DNA.
DR   PIR; A35347; A35347.
DR   RefSeq; YP_232968.1; NC_006998.1.
DR   IntAct; P68613; 3.
DR   MINT; P68613; -.
DR   DNASU; 3707542; -.
DR   GeneID; 3707542; -.
DR   KEGG; vg:3707542; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0039695; P:DNA-templated viral transcription; IDA:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR005022; Pox_TAP.
DR   Pfam; PF03355; Pox_TAP; 1.
PE   1: Evidence at protein level;
KW   Activator; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..260
FT                   /note="Late transcription factor 1"
FT                   /id="PRO_0000099167"
FT   MUTAGEN         60
FT                   /note="Missing: Essential for transactivation."
FT                   /evidence="ECO:0000269|PubMed:2024483"
SQ   SEQUENCE   260 AA;  29911 MW;  D2407C4A5CEF4D69 CRC64;
     MSIRIKIDKL RQIVAYFSEF SEEVSINVDS TDELMYIFAA LGGSVNIWAI IPLSASVFYR
     GAENIVFNLP VSKVKSCLCS FHNDAIIDIE PDLENNLVKL SSYHVVSVDC NKELMPIRTD
     TTICLSIDQK KSYVFNFHKY EEKCCGRTVI HLEWLLGFIK CISQHQHLAI MFKDDNIIMK
     TPGNTDAFSR EYSMTECSQE LQKFSFKIAI SSLNKLRGFK KRVNVFETRI VMDNDDNILG
     MLFSDRVQSF KINIFMAFLD