ID   VLTF2_VACCW             Reviewed;         150 AA.
AC   P07610; Q76ZR3;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   02-JUN-2021, entry version 85.
DE   RecName: Full=Viral late gene transcription factor 2;
DE            Short=VLTF-2;
DE   AltName: Full=Trans-activator protein A1;
GN   Name=VLTF2; OrderedLocusNames=VACWR119; ORFNames=A1L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3008103; DOI=10.1093/nar/14.7.3003;
RA   Weinrich S.L., Hruby D.E.;
RT   "A tandemly-oriented late gene cluster within the vaccinia virus genome.";
RL   Nucleic Acids Res. 14:3003-3016(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3811229; DOI=10.1016/0042-6822(87)90444-2;
RA   Baldick C.J. Jr., Moss B.;
RT   "Resistance of vaccinia virus to rifampicin conferred by a single
RT   nucleotide substitution near the predicted NH2 terminus of a gene encoding
RT   an Mr 62,000 polypeptide.";
RL   Virology 156:138-145(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1566576; DOI=10.1016/0042-6822(92)90753-c;
RA   Carpenter M.S., Delange A.M.;
RT   "Identification of a temperature-sensitive mutant of vaccinia virus
RT   defective in late but not intermediate gene expression.";
RL   Virology 188:233-244(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=2344616; DOI=10.1016/0092-8674(90)90190-p;
RA   Keck J.G., Baldick C.J. Jr., Moss B.;
RT   "Role of DNA replication in vaccinia virus gene expression: a naked
RT   template is required for transcription of three late trans-activator
RT   genes.";
RL   Cell 61:801-809(1990).
RN   [6]
RP   FUNCTION.
RX   PubMed=8371339; DOI=10.1128/jvi.67.10.5740-5748.1993;
RA   Keck J.G., Kovacs G.R., Moss B.;
RT   "Overexpression, purification, and late transcription factor activity of
RT   the 17-kilodalton protein encoded by the vaccinia virus A1L gene.";
RL   J. Virol. 67:5740-5748(1993).
RN   [7]
RP   INTERACTION WITH VLTF-1.
RX   PubMed=15518812; DOI=10.1016/j.virol.2004.08.017;
RA   Dellis S., Strickland K.C., McCrary W.J., Patel A., Stocum E., Wright C.F.;
RT   "Protein interactions among the vaccinia virus late transcription
RT   factors.";
RL   Virology 329:328-336(2004).
CC   -!- FUNCTION: Acts with RNA polymerase to initiate transcription from late
CC       gene promoters. {ECO:0000269|PubMed:8371339}.
CC   -!- SUBUNIT: Interacts with itself. Interacts with the late transcription
CC       factors VLTF-1. {ECO:0000269|PubMed:15518812}.
CC   -!- INTERACTION:
CC       P07610; P68613: VLTF1; NbExp=4; IntAct=EBI-7273273, EBI-7273218;
CC       P07610; P07610: VLTF2; NbExp=2; IntAct=EBI-7273273, EBI-7273273;
CC   -!- DEVELOPMENTAL STAGE: Intermediate stages of infection.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae VLTF-2 family.
CC       {ECO:0000305}.
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DR   EMBL; M16556; AAA48304.1; -; Genomic_DNA.
DR   EMBL; X03729; CAA27367.1; -; Genomic_DNA.
DR   EMBL; M86531; AAB59802.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89398.1; -; Genomic_DNA.
DR   PIR; C23768; WMVZR2.
DR   RefSeq; YP_233001.1; NC_006998.1.
DR   DIP; DIP-2161N; -.
DR   IntAct; P07610; 2.
DR   MINT; P07610; -.
DR   DNASU; 3707517; -.
DR   GeneID; 3707517; -.
DR   KEGG; vg:3707517; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0039695; P:DNA-templated viral transcription; IDA:UniProtKB.
DR   InterPro; IPR004975; Poxvirus_VLTF2.
DR   InterPro; IPR010507; Znf_MYM.
DR   Pfam; PF03295; Pox_TAA1; 1.
DR   Pfam; PF06467; zf-FCS; 1.
PE   1: Evidence at protein level;
KW   Activator; Late protein; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..150
FT                   /note="Viral late gene transcription factor 2"
FT                   /id="PRO_0000099172"
SQ   SEQUENCE   150 AA;  16906 MW;  5569B1B78F0A9A8D CRC64;
     MAKRVSLPDV VISAPKAVFK PAKEEALACI LPKYYKSMAD VSIKTNSVID KCWFCNQDLV
     FKPISIETFK GGEVGYFCSK ICRDSLASMV KSHVALREEP KISLLPLVFY EDKEKVINTI
     NLLRDKDGVY GSCYFKENSQ IIDISLRSLL