ID   VLTF3_VACCW             Reviewed;         224 AA.
AC   P68319; P07609;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   23-FEB-2022, entry version 58.
DE   RecName: Full=Viral late gene transcription factor 3;
DE            Short=VLTF-3;
DE   AltName: Full=Trans-activator protein A2;
GN   Name=VLTF3; OrderedLocusNames=VACWR120; ORFNames=A2L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3008103; DOI=10.1093/nar/14.7.3003;
RA   Weinrich S.L., Hruby D.E.;
RT   "A tandemly-oriented late gene cluster within the vaccinia virus genome.";
RL   Nucleic Acids Res. 14:3003-3016(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=2344616; DOI=10.1016/0092-8674(90)90190-p;
RA   Keck J.G., Baldick C.J. Jr., Moss B.;
RT   "Role of DNA replication in vaccinia virus gene expression: a naked
RT   template is required for transcription of three late trans-activator
RT   genes.";
RL   Cell 61:801-809(1990).
RN   [4]
RP   ZINC-FINGER, AND MUTAGENESIS OF CYS-6; CYS-9; CYS-23 AND CYS-26.
RX   PubMed=8371340; DOI=10.1128/jvi.67.10.5749-5753.1993;
RA   Keck J.G., Feigenbaum F., Moss B.;
RT   "Mutational analysis of a predicted zinc-binding motif in the 26-kilodalton
RT   protein encoded by the vaccinia virus A2L gene: correlation of zinc binding
RT   with late transcriptional transactivation activity.";
RL   J. Virol. 67:5749-5753(1993).
RN   [5]
RP   FUNCTION.
RX   PubMed=8676468; DOI=10.1128/jvi.70.7.4444-4450.1996;
RA   Passarelli A.L., Kovacs G.R., Moss B.;
RT   "Transcription of a vaccinia virus late promoter template: requirement for
RT   the product of the A2L intermediate-stage gene.";
RL   J. Virol. 70:4444-4450(1996).
RN   [6]
RP   INTERACTION WITH THE LATE TRANSCRIPTION ELONGATION FACTOR H5 AND VLTF-1.
RX   PubMed=15518812; DOI=10.1016/j.virol.2004.08.017;
RA   Dellis S., Strickland K.C., McCrary W.J., Patel A., Stocum E., Wright C.F.;
RT   "Protein interactions among the vaccinia virus late transcription
RT   factors.";
RL   Virology 329:328-336(2004).
CC   -!- FUNCTION: Acts with RNA polymerase to initiate transcription from late
CC       gene promoters. {ECO:0000269|PubMed:8676468}.
CC   -!- SUBUNIT: Interacts with the late transcription elongation factor
CC       H5/VLTF-4. Interacts with the late transcription factors VLTF-1.
CC       {ECO:0000269|PubMed:15518812}.
CC   -!- DEVELOPMENTAL STAGE: Intermediate stages of infection.
CC   -!- SIMILARITY: Belongs to the nucleo-cytoplasmic large DNA viruses
CC       (NCLDVs) VLTF-3 family. {ECO:0000305}.
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DR   EMBL; X03729; CAA27366.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89399.1; -; Genomic_DNA.
DR   PIR; B23768; WMVZR1.
DR   RefSeq; YP_233002.1; NC_006998.1.
DR   SMR; P68319; -.
DR   DNASU; 3707518; -.
DR   GeneID; 3707518; -.
DR   KEGG; vg:3707518; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0039695; P:DNA-templated viral transcription; IDA:UniProtKB.
DR   GO; GO:0046782; P:regulation of viral transcription; IEA:InterPro.
DR   InterPro; IPR007031; Poxvirus_VLTF3.
DR   InterPro; IPR014900; VLTF-3_Zn_ribbon.
DR   Pfam; PF08792; A2L_zn_ribbon; 1.
DR   Pfam; PF04947; Pox_VLTF3; 1.
PE   1: Evidence at protein level;
KW   Activator; Late protein; Metal-binding; Reference proteome; Transcription;
KW   Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..224
FT                   /note="Viral late gene transcription factor 3"
FT                   /id="PRO_0000099177"
FT   ZN_FING         6..26
FT                   /evidence="ECO:0000305"
FT   MUTAGEN         6
FT                   /note="C->S: Complete loss of transactivation."
FT                   /evidence="ECO:0000269|PubMed:8371340"
FT   MUTAGEN         9
FT                   /note="C->S: Complete loss of transactivation."
FT                   /evidence="ECO:0000269|PubMed:8371340"
FT   MUTAGEN         23
FT                   /note="C->S: Complete loss of transactivation."
FT                   /evidence="ECO:0000269|PubMed:8371340"
FT   MUTAGEN         26
FT                   /note="C->S: Complete loss of transactivation."
FT                   /evidence="ECO:0000269|PubMed:8371340"
SQ   SEQUENCE   224 AA;  26289 MW;  B2406504F65885FB CRC64;
     MNLRLCSGCR HNGIVSEQGY EYCIFCESVF QKCTKVQKKS NFHVSNKLIH LRNVLRRLLS
     HQCSGEIISE LLDIMEKNQI STDDVDANFV SSFLKANERI NKKDYKLVFE IINQVKDEKL
     NLSTEKINEV VEIFKHLVFF CQENTPSKTI NYSFFLDKIF DITSVTKNLK PQTVKNYTKN
     NSNQLVWENF LAHMRSKKRV TMVEDYGHEY VFVDERFSTC SLEV