VM1H2_CRORU
ID VM1H2_CRORU Reviewed; 202 AA.
AC P20897;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Snake venom metalloproteinase HT-2;
DE Short=SVMP;
DE EC=3.4.24.48;
DE AltName: Full=Hemorrhagic metalloproteinase HT-2;
DE AltName: Full=Hemorrhagic toxin II;
DE AltName: Full=Ruberlysin;
OS Crotalus ruber ruber (Red diamond rattlesnake).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Viperidae; Crotalinae; Crotalus.
OX NCBI_TaxID=8736;
RN [1]
RP PROTEIN SEQUENCE, PYROGLUTAMATE FORMATION AT GLN-1, AND DISULFIDE BONDS.
RC TISSUE=Venom;
RX PubMed=2081731; DOI=10.1093/oxfordjournals.jbchem.a123270;
RA Takeya H., Onikura T., Sugihara H., Iwanaga S.;
RT "Primary structure of a hemorrhagic metalloproteinase, HT-2, isolated from
RT the venom of Crotalus ruber ruber.";
RL J. Biochem. 108:711-719(1990).
CC -!- FUNCTION: This protein is a zinc protease from snake venom that induces
CC hemorrhage.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Cleavage of 10-His-|-Leu-11, 14-Ala-|-Leu-15, 16-Tyr-|-Leu-17
CC and 23-Gly-|-Phe-24 bonds of the B chain of insulin, His-|-Pro,
CC Pro-|-Phe, and Trp-|-Ser of angiotensin I, and Gly-|-Phe of Met
CC enkephalin.; EC=3.4.24.48;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC -!- SIMILARITY: Belongs to the venom metalloproteinase (M12B) family. P-I
CC subfamily. {ECO:0000305}.
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DR PIR; JX0139; HYRSR.
DR AlphaFoldDB; P20897; -.
DR SMR; P20897; -.
DR MEROPS; M12.150; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04269; ZnMc_adamalysin_II_like; 1.
DR Gene3D; 3.40.390.10; -; 1.
DR InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR InterPro; IPR001590; Peptidase_M12B.
DR InterPro; IPR034027; Reprolysin_adamalysin.
DR Pfam; PF01421; Reprolysin; 1.
DR PROSITE; PS50215; ADAM_MEPRO; 1.
DR PROSITE; PS00142; ZINC_PROTEASE; 1.
PE 1: Evidence at protein level;
KW Calcium; Direct protein sequencing; Disulfide bond; Hemorrhagic toxin;
KW Hemostasis impairing toxin; Hydrolase; Metal-binding; Metalloprotease;
KW Protease; Pyrrolidone carboxylic acid; Secreted; Toxin; Zinc.
FT CHAIN 1..202
FT /note="Snake venom metalloproteinase HT-2"
FT /id="PRO_0000078191"
FT DOMAIN 6..202
FT /note="Peptidase M12B"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00276"
FT ACT_SITE 143
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00276,
FT ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 9
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 93
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 142
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 146
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 152
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 197
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT BINDING 200
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250"
FT MOD_RES 1
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:2081731"
FT DISULFID 117..197
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00276,
FT ECO:0000269|PubMed:2081731"
FT DISULFID 157..164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00276,
FT ECO:0000269|PubMed:2081731"
SQ SEQUENCE 202 AA; 23321 MW; 5AF5D29318A61A9B CRC64;
QNLPQSYIEL VVVADHRMFM KYNSDLNTIR TRVHEIVNFI NEFYRSLNIR VSLTDLEIWS
DQDFITVQSS AKNTLHSFGE WRKSVLLNRK RHDNAQLLTA IVLDDYTLGL AYLNSMCHPR
NSVGLIQDHS PINLLMGVTM AHELGHNLGM EHDGKDCLRG ASLCIMRPGL TPGRSYEFSD
ASMRYYQKFL DQYKPQCILN KP
