VM26B_ECHCS
ID VM26B_ECHCS Reviewed; 69 AA.
AC P82466;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2000, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Disintegrin EC6 subunit beta;
DE Short=EC6B;
OS Echis carinatus sochureki (Saw-scaled viper).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Viperidae; Viperinae; Echis.
OX NCBI_TaxID=124223;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Venom;
RX PubMed=10926928; DOI=10.1074/jbc.m003209200;
RA Marcinkiewicz C., Taooka Y., Yokosaki Y., Calvete J.J., Marcinkiewicz M.M.,
RA Lobb R.R., Niewiarowski S., Sheppard D.;
RT "Inhibitory effects of MLDG-containing heterodimeric disintegrins reveal
RT distinct structural requirements for interaction of the integrin alpha
RT 9beta 1 with VCAM-1, tenascin-C, and osteopontin.";
RL J. Biol. Chem. 275:31930-31937(2000).
CC -!- FUNCTION: Potently inhibits adhesion of alpha-4/beta-1 (ITGA4/ITGB1)
CC and alpha-9/beta-1 (ITGA9/ITGB1) integrins to VCAM1, and adhesion of
CC alpha-5/beta-1 (ITGA5/ITGB1) integrin to fibronectin. Has a much less
CC effect on alpha-IIb/beta-3 (ITGA2B/ITGB3) integrin. Also potently
CC inhibits neutrophil migration across TNF-alpha-activated human
CC umbilical endothelial cells. {ECO:0000269|PubMed:10926928}.
CC -!- SUBUNIT: Heterodimer with subunit alpha; disulfide-linked.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC -!- MISCELLANEOUS: The disintegrin belongs to the dimeric disintegrin
CC subfamily.
CC -!- SIMILARITY: Belongs to the venom metalloproteinase (M12B) family. P-II
CC subfamily. P-IIe sub-subfamily. {ECO:0000305}.
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DR AlphaFoldDB; P82466; -.
DR SMR; P82466; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 4.10.70.10; -; 1.
DR InterPro; IPR018358; Disintegrin_CS.
DR InterPro; IPR001762; Disintegrin_dom.
DR InterPro; IPR036436; Disintegrin_dom_sf.
DR Pfam; PF00200; Disintegrin; 1.
DR PRINTS; PR00289; DISINTEGRIN.
DR SMART; SM00050; DISIN; 1.
DR SUPFAM; SSF57552; SSF57552; 1.
DR PROSITE; PS00427; DISINTEGRIN_1; 1.
DR PROSITE; PS50214; DISINTEGRIN_2; 1.
PE 1: Evidence at protein level;
KW Cell adhesion impairing toxin; Direct protein sequencing; Disulfide bond;
KW Secreted; Toxin.
FT CHAIN 1..69
FT /note="Disintegrin EC6 subunit beta"
FT /id="PRO_0000101802"
FT DOMAIN 1..65
FT /note="Disintegrin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT MOTIF 42..44
FT /note="Cell attachment site"
FT DISULFID 6..29
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT DISULFID 7
FT /note="Interchain (with C-59 in subunit alpha)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT DISULFID 12
FT /note="Interchain (with C-54 in subunit alpha)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT DISULFID 20..26
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT DISULFID 25..50
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT DISULFID 38..57
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
SQ SEQUENCE 69 AA; 7279 MW; 6916F2CA0821E1E8 CRC64;
NSVHPCCDPV TCKPKRGKHC ASGPCCENCY IVGVGTVCNP ARGDWNDDNC TGVSSDCPPN
PWNGKPSDN