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VM2L4_GLOBR
ID   VM2L4_GLOBR             Reviewed;         319 AA.
AC   Q698K8;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2013, sequence version 3.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Zinc metalloproteinase/disintegrin;
DE   Contains:
DE     RecName: Full=Snake venom metalloproteinase brevilysin L4;
DE              Short=SVMP;
DE     AltName: Full=Snake venom metalloproteinase hxl-1;
DE              EC=3.4.24.-;
DE   Contains:
DE     RecName: Full=Disintegrin brevicaudin-1a;
DE   Contains:
DE     RecName: Full=Disintegrin brevicaudin-1b;
DE     AltName: Full=Disintegrin adinbitor;
DE     AltName: Full=Disintegrin halystatin;
DE   Flags: Precursor; Fragment;
OS   Gloydius brevicaudus (Korean slamosa snake) (Agkistrodon halys
OS   brevicaudus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Gloydius.
OX   NCBI_TaxID=259325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-237, MASS SPECTROMETRY,
RP   AND 3D-STRUCTURE MODELING.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=15777952; DOI=10.1016/j.toxicon.2004.12.005;
RA   Deshimaru M., Ichihara M., Hattori T., Koba K., Terada S.;
RT   "Primary structure of brevilysin L4, an enzymatically active fragment of a
RT   disintegrin precursor from Gloydius halys brevicaudus venom.";
RL   Toxicon 45:571-580(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 244-319, FUNCTION, AND ACTIVITY REGULATION.
RC   TISSUE=Venom gland;
RX   PubMed=15188058; DOI=10.1093/abbs/36.6.425;
RA   Wang J.-H., Wu Y., Ren F., Lu L., Zhao B.-C.;
RT   "Cloning and characterization of adinbitor, a novel disintegrin from the
RT   snake venom of Agkistrodon halys brevicaudus stejneger.";
RL   Acta Biochim. Biophys. Sin. 36:425-429(2004).
RN   [3]
RP   PROTEIN SEQUENCE OF 247-319, AND FUNCTION.
RC   TISSUE=Venom;
RA   Oshikawa K., Yasukouchi Y., Terada S.;
RT   "Isolation and primary structures of platelet aggregation inhibitors from
RT   Gloydius halys brevicaudus venom.";
RL   Fukuoka Univ. Sci. Rep. 30:201-208(2000).
CC   -!- FUNCTION: [Snake venom metalloproteinase brevilysin L4]:
CC       metalloproteinase that impairs hemostasis in the envenomed animal (By
CC       similarity). Shows autoproteolysis dependent on pH and temperature.
CC       Does not show hemorrhagic activity. {ECO:0000250,
CC       ECO:0000269|PubMed:15188058, ECO:0000269|Ref.3}.
CC   -!- FUNCTION: [Disintegrin brevicaudin-1a]: Inhibits platelet aggregation
CC       induced by ADP (IC(50) is 200 nM), collagen (IC(50) is 500 nM),
CC       thrombin and epinephrin (IC(50) is 300 nM). Does not inhibit
CC       aggregation induced by ristocetin.
CC   -!- FUNCTION: [Disintegrin brevicaudin-1b]: Inhibits platelet aggregation
CC       induced by ADP (IC(50) is 100 nM), collagen (IC(50) is 500 nM),
CC       thrombin and epinephrin (IC(50) is 300 nM). Does not inhibit
CC       aggregation induced by ristocetin. Significantly inhibits angiogenesis
CC       both in vivo and in vitro.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- ACTIVITY REGULATION: Excess of calcium ions significantly suppress the
CC       autoproteolysis of the enzyme. {ECO:0000269|PubMed:15188058}.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- MASS SPECTROMETRY: [Snake venom metalloproteinase brevilysin L4]:
CC       Mass=22752; Method=MALDI; Evidence={ECO:0000269|PubMed:15777952};
CC   -!- MISCELLANEOUS: The disintegrins belong to the medium disintegrin
CC       subfamily.
CC   -!- SIMILARITY: Belongs to the venom metalloproteinase (M12B) family. P-II
CC       subfamily. P-IIa sub-subfamily. {ECO:0000305}.
CC   -!- CAUTION: The metalloprotease is also encoded by another precursor (AC
CC       Q90WC0). {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAT76292.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AY551929; AAT76292.1; ALT_TERM; mRNA.
DR   PIR; A59410; A59410.
DR   PIR; A59411; A59411.
DR   AlphaFoldDB; Q698K8; -.
DR   SMR; Q698K8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd04269; ZnMc_adamalysin_II_like; 1.
DR   Gene3D; 3.40.390.10; -; 1.
DR   Gene3D; 4.10.70.10; -; 1.
DR   InterPro; IPR018358; Disintegrin_CS.
DR   InterPro; IPR001762; Disintegrin_dom.
DR   InterPro; IPR036436; Disintegrin_dom_sf.
DR   InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR   InterPro; IPR001590; Peptidase_M12B.
DR   InterPro; IPR034027; Reprolysin_adamalysin.
DR   Pfam; PF00200; Disintegrin; 1.
DR   Pfam; PF01421; Reprolysin; 1.
DR   PRINTS; PR00289; DISINTEGRIN.
DR   SMART; SM00050; DISIN; 1.
DR   SUPFAM; SSF57552; SSF57552; 1.
DR   PROSITE; PS50215; ADAM_MEPRO; 1.
DR   PROSITE; PS00427; DISINTEGRIN_1; 1.
DR   PROSITE; PS50214; DISINTEGRIN_2; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   1: Evidence at protein level;
KW   Calcium; Cell adhesion impairing toxin; Direct protein sequencing;
KW   Disulfide bond; Hemostasis impairing toxin; Hydrolase; Metal-binding;
KW   Metalloprotease; Platelet aggregation inhibiting toxin; Protease; Secreted;
KW   Toxin; Zinc; Zymogen.
FT   PROPEP          <1..28
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000424444"
FT   CHAIN           29..230
FT                   /note="Snake venom metalloproteinase brevilysin L4"
FT                   /id="PRO_0000424445"
FT   PROPEP          231..246
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000424446"
FT   CHAIN           247..319
FT                   /note="Disintegrin brevicaudin-1b"
FT                   /id="PRO_0000318180"
FT   CHAIN           249..319
FT                   /note="Disintegrin brevicaudin-1a"
FT                   /id="PRO_0000424447"
FT   DOMAIN          34..230
FT                   /note="Peptidase M12B"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00276"
FT   DOMAIN          238..319
FT                   /note="Disintegrin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00068"
FT   MOTIF           297..299
FT                   /note="Cell attachment site"
FT   ACT_SITE        171
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00276,
FT                   ECO:0000255|PROSITE-ProRule:PRU10095"
FT   BINDING         37
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         121
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         170
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         174
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         180
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         225
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         228
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   DISULFID        145..225
FT                   /evidence="ECO:0000250"
FT   DISULFID        185..209
FT                   /evidence="ECO:0000250"
FT   DISULFID        187..192
FT                   /evidence="ECO:0000250"
FT   DISULFID        252..261
FT                   /evidence="ECO:0000250"
FT   DISULFID        254..262
FT                   /evidence="ECO:0000250"
FT   DISULFID        267..281
FT                   /evidence="ECO:0000250"
FT   DISULFID        275..305
FT                   /evidence="ECO:0000250"
FT   DISULFID        280..284
FT                   /evidence="ECO:0000250"
FT   DISULFID        293..312
FT                   /evidence="ECO:0000250"
FT   CONFLICT        244..246
FT                   /note="ELL -> MHM (in Ref. 2; AAT76292)"
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
SQ   SEQUENCE   319 AA;  35314 MW;  093F991FA589B1FA CRC64;
     EDEAPKMCGV TQNWESYEPI KKASQSNLTP AHQRYIELVI VADHGMFTKY NGDSDKIREW
     VRQMVNTVDE IYSYMYIDVA LAGLEIWSNE DLINVQPAAP HTLDSFGKWR ERDLLHRIHH
     DNAMLLTAID FDGPTIGLAY VGTMCKPKGS TGVVQDHSTI NLRVAVTMAH EIGHNLGIHH
     DTGSCSCGGY SCIMSPVISH EPSKYFSDCS YTQCWDFIMN QKPQCILNKP LRTDTVSTPV
     SGNELLEAGE ECDCGSPGNP CCDAATCKLR QGAQCAEGLC CDQCRFMKKG TVCRIARGDD
     MDDYCNGISA GCPRNPFHA
 
 
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