CALI_HUMAN
ID CALI_HUMAN Reviewed; 588 AA.
AC Q13939; Q9BXG7;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 3.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Calicin;
GN Name=CCIN;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RA Sha J.H., Zhou Z.M., Li J.M.;
RL Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-588, AND TISSUE SPECIFICITY.
RC TISSUE=Sperm;
RX PubMed=7641791; DOI=10.1006/excr.1995.1246;
RA von Buelow M., Heid H.W., Hess H., Franke W.W.;
RT "Molecular nature of calicin, a major basic protein of the mammalian sperm
RT head cytoskeleton.";
RL Exp. Cell Res. 219:407-413(1995).
CC -!- FUNCTION: Possible morphogenetic cytoskeletal element in spermiogenic
CC differentiation.
CC -!- INTERACTION:
CC Q13939; P42858: HTT; NbExp=3; IntAct=EBI-25879469, EBI-466029;
CC Q13939; O60260-5: PRKN; NbExp=3; IntAct=EBI-25879469, EBI-21251460;
CC Q13939; Q13148: TARDBP; NbExp=3; IntAct=EBI-25879469, EBI-372899;
CC Q13939; P40337-2: VHL; NbExp=3; IntAct=EBI-25879469, EBI-12157263;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, perinuclear theca,
CC calyx. Note=Sperm head cytoskeletal structure tightly associated to the
CC nucleus.
CC -!- TISSUE SPECIFICITY: Testis. Not detectable or shows a drastically
CC altered pattern of arrangement in the heads of malformed spermatozoa.
CC {ECO:0000269|PubMed:7641791}.
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DR EMBL; AF333334; AAK20829.1; -; mRNA.
DR EMBL; Z46967; CAA87088.1; -; mRNA.
DR CCDS; CCDS6599.1; -.
DR PIR; I37216; I37216.
DR RefSeq; NP_005884.2; NM_005893.2.
DR AlphaFoldDB; Q13939; -.
DR SMR; Q13939; -.
DR BioGRID; 107324; 10.
DR IntAct; Q13939; 4.
DR STRING; 9606.ENSP00000334996; -.
DR iPTMnet; Q13939; -.
DR PhosphoSitePlus; Q13939; -.
DR BioMuta; CCIN; -.
DR DMDM; 143811369; -.
DR MassIVE; Q13939; -.
DR PaxDb; Q13939; -.
DR PeptideAtlas; Q13939; -.
DR PRIDE; Q13939; -.
DR ProteomicsDB; 59761; -.
DR Antibodypedia; 11871; 137 antibodies from 22 providers.
DR DNASU; 881; -.
DR Ensembl; ENST00000335119.4; ENSP00000334996.2; ENSG00000185972.6.
DR GeneID; 881; -.
DR KEGG; hsa:881; -.
DR MANE-Select; ENST00000335119.4; ENSP00000334996.2; NM_005893.3; NP_005884.2.
DR CTD; 881; -.
DR GeneCards; CCIN; -.
DR HGNC; HGNC:1568; CCIN.
DR HPA; ENSG00000185972; Tissue enriched (testis).
DR MIM; 603960; gene.
DR neXtProt; NX_Q13939; -.
DR OpenTargets; ENSG00000185972; -.
DR PharmGKB; PA26140; -.
DR VEuPathDB; HostDB:ENSG00000185972; -.
DR eggNOG; KOG4441; Eukaryota.
DR GeneTree; ENSGT00940000162268; -.
DR HOGENOM; CLU_466589_0_0_1; -.
DR InParanoid; Q13939; -.
DR OMA; MPYKAAA; -.
DR OrthoDB; 311575at2759; -.
DR PhylomeDB; Q13939; -.
DR TreeFam; TF331981; -.
DR PathwayCommons; Q13939; -.
DR SignaLink; Q13939; -.
DR SIGNOR; Q13939; -.
DR BioGRID-ORCS; 881; 23 hits in 1111 CRISPR screens.
DR ChiTaRS; CCIN; human.
DR GenomeRNAi; 881; -.
DR Pharos; Q13939; Tbio.
DR PRO; PR:Q13939; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q13939; protein.
DR Bgee; ENSG00000185972; Expressed in left testis and 92 other tissues.
DR ExpressionAtlas; Q13939; baseline and differential.
DR Genevisible; Q13939; HS.
DR GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR GO; GO:0033150; C:cytoskeletal calyx; IEA:UniProtKB-SubCell.
DR GO; GO:0032839; C:dendrite cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0030036; P:actin cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR Gene3D; 2.120.10.80; -; 2.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR017096; BTB-kelch_protein.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF01344; Kelch_1; 1.
DR PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00612; Kelch; 3.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Developmental protein; Differentiation;
KW Kelch repeat; Phosphoprotein; Reference proteome; Repeat; Spermatogenesis.
FT CHAIN 1..588
FT /note="Calicin"
FT /id="PRO_0000119067"
FT DOMAIN 12..124
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 133..235
FT /note="BACK"
FT REPEAT 280..327
FT /note="Kelch 1"
FT REPEAT 328..375
FT /note="Kelch 2"
FT REPEAT 377..423
FT /note="Kelch 3"
FT REPEAT 425..475
FT /note="Kelch 4"
FT REPEAT 476..525
FT /note="Kelch 5"
FT REPEAT 526..580
FT /note="Kelch 6"
FT MOD_RES 149
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5XI58"
FT VARIANT 75
FT /note="S -> N (in dbSNP:rs34789048)"
FT /id="VAR_050039"
FT CONFLICT 226
FT /note="N -> S (in Ref. 1; AAK20829)"
FT /evidence="ECO:0000305"
FT CONFLICT 433
FT /note="C -> W (in Ref. 2; CAA87088)"
FT /evidence="ECO:0000305"
FT CONFLICT 490
FT /note="I -> N (in Ref. 1; AAK20829)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 588 AA; 66582 MW; 8EEB910611BBA98B CRC64;
MKLEFTEKNY NSFVLQNLNR QRKRKEYWDM ALSVDNHVFF AHRNVLAAVS PLVRSLISSN
DMKTADELFI TIDTSYLSPV TVDQLLDYFY SGKVVISEQN VEELLRGAQY FNTPRLRVHC
NDFLIKSICR ANCLRYLFLA ELFELKEVSD VAYSGIRDNF HYWASPEGSM HFMRCPPVIF
GRLLRDENLH VLNEDQALSA LINWVYFRKE DREKYFKKFF NYINLNAVSN KTLVFASNKL
VGMENTSSHT TLIESVLMDR KQERPCSLLV YQRKGALLDS VVILGGQKAH GQFNDGVFAY
IIQENLWMKL SDMPYRAAAL SATSAGRYIY ISGGTTEQIS GLKTAWRYDM DDNSWTKLPD
LPIGLVFHTM VTCGGTVYSV GGSIAPRRYV SNIYRYDERK EVWCLAGKMS IPMDGTAVIT
KGDRHLYIVT GRCLVKGYIS RVGVVDCFDT STGDVVQCIT FPIEFNHRPL LSFQQDNILC
VHSHRQSVEI NLQKVKASKT TTSVPVLPNS CPLDVSHAIC SIGDSKVFVC GGVTTASDVQ
TKDYTINPNA FLLDQKTGKW KTLAPPPEAL DCPACCLAKL PCKILQRI
