ID   VM3AH_NAJAT             Reviewed;          16 AA.
AC   P0DJJ1;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2012, sequence version 1.
DT   03-AUG-2022, entry version 22.
DE   RecName: Full=Zinc metalloproteinase atrahagin;
DE            EC=3.4.24.-;
DE   AltName: Full=Snake venom metalloproteinase;
DE            Short=SVMP;
DE   Flags: Fragment;
OS   Naja atra (Chinese cobra).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8656;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, ACTIVITY REGULATION, AND SUBUNIT.
RC   TISSUE=Venom;
RX   PubMed=16310401; DOI=10.1016/j.biocel.2005.10.011;
RA   Wei J.-F., Mo Y.-Z., Qiao L.-Y., Wei X.-L., Chen H.-Q., Xie H., Fu Y.-L.,
RA   Wang W.-Y., Xiong Y.-L., He S.-H.;
RT   "Potent histamine-releasing activity of atrahagin, a novel snake venom
RT   metalloproteinase.";
RL   Int. J. Biochem. Cell Biol. 38:510-520(2006).
CC   -!- FUNCTION: Snake venom zinc metalloprotease that causes mast cell
CC       degranulation and histamine release. Selectively degrades the alpha-
CC       chain of human fibrinogen (FGA). {ECO:0000269|PubMed:16310401}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- ACTIVITY REGULATION: Inhibited by EDTA and EGTA, but not by PMSF and
CC       leupeptin. {ECO:0000269|PubMed:16310401}.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:16310401}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- PTM: Glycosylated. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Does not degrade the beta- and the gamma-chain of
CC       fibrinogen. Does not induce skin hemorrhage when subcutaneously
CC       injected into mice (5 or 50 ug) (PubMed:16310401).
CC       {ECO:0000305|PubMed:16310401}.
CC   -!- SIMILARITY: Belongs to the venom metalloproteinase (M12B) family. P-III
CC       subfamily. P-IIIa sub-subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DJJ1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Fibrinogenolytic toxin; Glycoprotein;
KW   Hemostasis impairing toxin; Hydrolase; Metal-binding; Metalloprotease;
KW   Protease; Secreted; Toxin; Zinc.
FT   CHAIN           1..>16
FT                   /note="Zinc metalloproteinase atrahagin"
FT                   /id="PRO_0000418046"
FT   DOMAIN          14..>16
FT                   /note="Peptidase M12B"
FT   NON_TER         16
SQ   SEQUENCE   16 AA;  2005 MW;  0C4542DA0EE68F8D CRC64;
     TNTPEDDRYL QDYVYI