CALL4_HUMAN
ID CALL4_HUMAN Reviewed; 196 AA.
AC Q96GE6; B4DL15; F8W6Y4; Q6MZY3; Q6N048; Q9H286;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 3.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Calmodulin-like protein 4;
DE AltName: Full=Serologically defined breast cancer antigen NY-BR-20;
GN Name=CALML4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC TISSUE=Mammary gland;
RX PubMed=12747765;
RA Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O.,
RA Jaeger D., Chen Y.-T., Mackay A., O'Hare M.J., Old L.J.;
RT "Humoral immunity to human breast cancer: antigen definition and
RT quantitative analysis of mRNA expression.";
RL Cancer Immun. 1:4-4(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Placenta;
RA Li W.B., Gruber C., Jessee J., Polayes D.;
RT "Full-length cDNA libraries and normalization.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5).
RC TISSUE=Prostate, and Uterine endothelium;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 16-196 (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q96GE6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96GE6-2; Sequence=VSP_030436;
CC Name=3;
CC IsoId=Q96GE6-3; Sequence=VSP_030435;
CC Name=4;
CC IsoId=Q96GE6-4; Sequence=VSP_047150;
CC Name=5;
CC IsoId=Q96GE6-5; Sequence=VSP_055513, VSP_055514;
CC -!- TISSUE SPECIFICITY: Expressed in breast cancer cell lines.
CC {ECO:0000269|PubMed:12747765}.
CC -!- SIMILARITY: Belongs to the calmodulin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG48255.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAG59377.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF308287; AAG48255.1; ALT_SEQ; mRNA.
DR EMBL; CR598967; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BX640702; CAE45822.1; -; mRNA.
DR EMBL; BX640817; CAE45894.1; -; mRNA.
DR EMBL; AC107871; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471082; EAW77810.1; -; Genomic_DNA.
DR EMBL; BC009516; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK296804; BAG59377.1; ALT_INIT; mRNA.
DR CCDS; CCDS66808.1; -. [Q96GE6-2]
DR RefSeq; NP_001026903.2; NM_001031733.2.
DR RefSeq; NP_001273623.1; NM_001286694.1. [Q96GE6-2]
DR RefSeq; NP_001273624.1; NM_001286695.1. [Q96GE6-3]
DR RefSeq; NP_219501.2; NM_033429.2.
DR AlphaFoldDB; Q96GE6; -.
DR SMR; Q96GE6; -.
DR BioGRID; 124886; 2.
DR IntAct; Q96GE6; 1.
DR STRING; 9606.ENSP00000419081; -.
DR iPTMnet; Q96GE6; -.
DR PhosphoSitePlus; Q96GE6; -.
DR BioMuta; CALML4; -.
DR DMDM; 209572745; -.
DR EPD; Q96GE6; -.
DR jPOST; Q96GE6; -.
DR MassIVE; Q96GE6; -.
DR MaxQB; Q96GE6; -.
DR PaxDb; Q96GE6; -.
DR PeptideAtlas; Q96GE6; -.
DR PRIDE; Q96GE6; -.
DR ProteomicsDB; 29855; -.
DR ProteomicsDB; 76627; -. [Q96GE6-1]
DR ProteomicsDB; 76628; -. [Q96GE6-2]
DR ProteomicsDB; 76629; -. [Q96GE6-3]
DR Antibodypedia; 42938; 133 antibodies from 22 providers.
DR DNASU; 91860; -.
DR Ensembl; ENST00000395463.3; ENSP00000435285.2; ENSG00000129007.16. [Q96GE6-2]
DR Ensembl; ENST00000540479.6; ENSP00000438177.2; ENSG00000129007.16. [Q96GE6-3]
DR GeneID; 91860; -.
DR KEGG; hsa:91860; -.
DR UCSC; uc002arb.4; human. [Q96GE6-1]
DR CTD; 91860; -.
DR GeneCards; CALML4; -.
DR HGNC; HGNC:18445; CALML4.
DR HPA; ENSG00000129007; Tissue enhanced (intestine).
DR neXtProt; NX_Q96GE6; -.
DR OpenTargets; ENSG00000129007; -.
DR PharmGKB; PA134903345; -.
DR VEuPathDB; HostDB:ENSG00000129007; -.
DR eggNOG; KOG0027; Eukaryota.
DR GeneTree; ENSGT00940000157859; -.
DR HOGENOM; CLU_2995949_0_0_1; -.
DR InParanoid; Q96GE6; -.
DR OMA; RMTDKQN; -.
DR OrthoDB; 1386217at2759; -.
DR PhylomeDB; Q96GE6; -.
DR TreeFam; TF300912; -.
DR PathwayCommons; Q96GE6; -.
DR BioGRID-ORCS; 91860; 7 hits in 1074 CRISPR screens.
DR ChiTaRS; CALML4; human.
DR GenomeRNAi; 91860; -.
DR Pharos; Q96GE6; Tdark.
DR PRO; PR:Q96GE6; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q96GE6; protein.
DR Bgee; ENSG00000129007; Expressed in mucosa of transverse colon and 93 other tissues.
DR ExpressionAtlas; Q96GE6; baseline and differential.
DR Genevisible; Q96GE6; HS.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR CDD; cd00051; EFh; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 2.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Repeat.
FT CHAIN 1..196
FT /note="Calmodulin-like protein 4"
FT /id="PRO_0000314933"
FT DOMAIN 51..86
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 87..122
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 124..159
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 160..195
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 1..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..115
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_030435"
FT VAR_SEQ 1..76
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.2"
FT /id="VSP_030436"
FT VAR_SEQ 45..59
FT /note="AKFLSQDQINEYKEC -> WMISSGKQISNPMAK (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_055513"
FT VAR_SEQ 55..101
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_047150"
FT VAR_SEQ 60..196
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_055514"
FT VARIANT 28
FT /note="R -> C (in dbSNP:rs3803381)"
FT /id="VAR_048586"
FT VARIANT 154
FT /note="T -> K (in dbSNP:rs2280217)"
FT /id="VAR_048587"
FT CONFLICT 40
FT /note="R -> G (in Ref. 1; AAG48255)"
FT /evidence="ECO:0000305"
FT CONFLICT 60
FT /note="F -> S (in Ref. 1; AAG48255)"
FT /evidence="ECO:0000305"
FT CONFLICT 76
FT /note="L -> F (in Ref. 1; AAG48255)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 196 AA; 21883 MW; 547C558AC7EDFABD CRC64;
MAAEHLLPGP PPSLADFRLE AGGKGTERGS GSSKPTGSSR GPRMAKFLSQ DQINEYKECF
SLYDKQQRGK IKATDLMVAM RCLGASPTPG EVQRHLQTHG IDGNGELDFS TFLTIMHMQI
KQEDPKKEIL LAMLMVDKEK KGYVMASDLR SKLTSLGEKL THKEVDDLFR EADIEPNGKV
KYDEFIHKIT LPGRDY
