ID   VMA21_CRYNB             Reviewed;         104 AA.
AC   P0CS25; Q55JN0; Q5K9X1;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Vacuolar ATPase assembly integral membrane protein VMA21 {ECO:0000255|HAMAP-Rule:MF_03058};
GN   Name=VMA21 {ECO:0000255|HAMAP-Rule:MF_03058}; OrderedLocusNames=CNBK3030;
OS   Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS   (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=283643;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B-3501A;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Required for the assembly of the V0 complex of the vacuolar
CC       ATPase (V-ATPase) in the endoplasmic reticulum. {ECO:0000255|HAMAP-
CC       Rule:MF_03058}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000255|HAMAP-Rule:MF_03058}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_03058}. Endoplasmic reticulum-Golgi
CC       intermediate compartment membrane {ECO:0000255|HAMAP-Rule:MF_03058};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03058}.
CC       Cytoplasmic vesicle, COPII-coated vesicle membrane {ECO:0000255|HAMAP-
CC       Rule:MF_03058}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_03058}.
CC   -!- SIMILARITY: Belongs to the VMA21 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03058}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAL17952.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AAEY01000053; EAL17952.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_772599.1; XM_767506.1.
DR   AlphaFoldDB; P0CS25; -.
DR   EnsemblFungi; EAL17952; EAL17952; CNBK3030.
DR   GeneID; 4939003; -.
DR   KEGG; cnb:CNBK3030; -.
DR   HOGENOM; CLU_154717_0_0_1; -.
DR   Proteomes; UP000001435; Chromosome 11.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_03058; VMA21; 1.
DR   InterPro; IPR019013; Vma21.
DR   PANTHER; PTHR31792; PTHR31792; 1.
DR   Pfam; PF09446; VMA21; 1.
PE   3: Inferred from homology;
KW   Cytoplasmic vesicle; Endoplasmic reticulum; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..104
FT                   /note="Vacuolar ATPase assembly integral membrane protein
FT                   VMA21"
FT                   /id="PRO_0000410325"
FT   TOPO_DOM        1..21
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TOPO_DOM        43..65
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TOPO_DOM        87..104
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   MOTIF           101..104
FT                   /note="Prevents secretion from ER"
SQ   SEQUENCE   104 AA;  10993 MW;  89F1FB413DDE2153 CRC64;
     MSNRVSTGKM AMAPQESVQP AVLYKLVLFA LLMAVVPIGT YFSTLNYLWD GASRCGFPSG
     LCSTTFAAIS AIAAANLILV GYVVVAFRED AASRTGPLPE KKTS