ID   VMA21_KLULA             Reviewed;          77 AA.
AC   Q6CQP9;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 2.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Vacuolar ATPase assembly integral membrane protein VMA21 {ECO:0000255|HAMAP-Rule:MF_03058};
GN   Name=VMA21 {ECO:0000255|HAMAP-Rule:MF_03058};
GN   OrderedLocusNames=KLLA0D15312g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for the assembly of the V0 complex of the vacuolar
CC       ATPase (V-ATPase) in the endoplasmic reticulum. {ECO:0000255|HAMAP-
CC       Rule:MF_03058}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000255|HAMAP-Rule:MF_03058}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_03058}. Endoplasmic reticulum-Golgi
CC       intermediate compartment membrane {ECO:0000255|HAMAP-Rule:MF_03058};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03058}.
CC       Cytoplasmic vesicle, COPII-coated vesicle membrane {ECO:0000255|HAMAP-
CC       Rule:MF_03058}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_03058}.
CC   -!- SIMILARITY: Belongs to the VMA21 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03058}.
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DR   EMBL; CR382124; CAH00836.2; -; Genomic_DNA.
DR   RefSeq; XP_453740.2; XM_453740.2.
DR   AlphaFoldDB; Q6CQP9; -.
DR   STRING; 28985.XP_453740.2; -.
DR   EnsemblFungi; CAH00836; CAH00836; KLLA0_D15312g.
DR   GeneID; 2892788; -.
DR   KEGG; kla:KLLA0_D15312g; -.
DR   eggNOG; ENOG502SBNA; Eukaryota.
DR   HOGENOM; CLU_154717_1_0_1; -.
DR   InParanoid; Q6CQP9; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_03058; VMA21; 1.
DR   InterPro; IPR019013; Vma21.
DR   PANTHER; PTHR31792; PTHR31792; 1.
DR   Pfam; PF09446; VMA21; 1.
PE   3: Inferred from homology;
KW   Cytoplasmic vesicle; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..77
FT                   /note="Vacuolar ATPase assembly integral membrane protein
FT                   VMA21"
FT                   /id="PRO_0000377589"
FT   TOPO_DOM        1..8
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TOPO_DOM        30..38
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TOPO_DOM        60..77
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   MOTIF           74..77
FT                   /note="Prevents secretion from ER"
SQ   SEQUENCE   77 AA;  8509 MW;  B6F5A70F51F7F439 CRC64;
     MPVDVPRSVI AKLMFFTVAM VVLPVLTFFW LQEHTDNTLV SGGLAAAMAN LVLIAYVIMA
     FQEDSSSSED DETKKEK