VMA21_MOUSE
ID VMA21_MOUSE Reviewed; 101 AA.
AC Q78T54; Q3UUC3;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Vacuolar ATPase assembly integral membrane protein Vma21 {ECO:0000255|HAMAP-Rule:MF_03058};
GN Name=Vma21;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=NMRI;
RX PubMed=16503986; DOI=10.1186/1471-2164-7-29;
RA Kolb-Kokocinski A., Mehrle A., Bechtel S., Simpson J.C., Kioschis P.,
RA Wiemann S., Wellenreuther R., Poustka A.;
RT "The systematic functional characterisation of Xq28 genes prioritises
RT candidate disease genes.";
RL BMC Genomics 7:29-29(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J;
RC TISSUE=Embryo, Embryonic stem cell, Hippocampus, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Mammary tumor, and Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Required for the assembly of the V0 complex of the vacuolar
CC ATPase (V-ATPase) in the endoplasmic reticulum. {ECO:0000255|HAMAP-
CC Rule:MF_03058}.
CC -!- SUBUNIT: Associates with the V0 complex of the vacuolar ATPase (V-
CC ATPase) (By similarity). Interacts with ATP6AP2 (By similarity).
CC {ECO:0000250|UniProtKB:Q3ZAQ7, ECO:0000255|HAMAP-Rule:MF_03058}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000255|HAMAP-Rule:MF_03058}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_03058}. Endoplasmic reticulum-Golgi
CC intermediate compartment membrane {ECO:0000255|HAMAP-Rule:MF_03058};
CC Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03058}.
CC Cytoplasmic vesicle, COPII-coated vesicle membrane {ECO:0000255|HAMAP-
CC Rule:MF_03058}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_03058}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q78T54-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q78T54-2; Sequence=VSP_037464;
CC -!- SIMILARITY: Belongs to the VMA21 family. {ECO:0000255|HAMAP-
CC Rule:MF_03058}.
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DR EMBL; AJ748652; CAG38686.1; -; mRNA.
DR EMBL; AK049296; BAC33664.1; -; mRNA.
DR EMBL; AK049488; BAC33774.1; -; mRNA.
DR EMBL; AK164163; BAE37657.1; -; mRNA.
DR EMBL; AK138582; BAE23704.1; -; mRNA.
DR EMBL; BC028317; AAH28317.1; -; mRNA.
DR EMBL; BC094639; AAH94639.1; -; mRNA.
DR CCDS; CCDS41000.1; -. [Q78T54-1]
DR RefSeq; NP_001074825.1; NM_001081356.3. [Q78T54-1]
DR RefSeq; NP_001277710.1; NM_001290781.1.
DR AlphaFoldDB; Q78T54; -.
DR SMR; Q78T54; -.
DR STRING; 10090.ENSMUSP00000110223; -.
DR iPTMnet; Q78T54; -.
DR PhosphoSitePlus; Q78T54; -.
DR EPD; Q78T54; -.
DR jPOST; Q78T54; -.
DR MaxQB; Q78T54; -.
DR PaxDb; Q78T54; -.
DR PeptideAtlas; Q78T54; -.
DR PRIDE; Q78T54; -.
DR ProteomicsDB; 300171; -. [Q78T54-1]
DR ProteomicsDB; 300172; -. [Q78T54-2]
DR TopDownProteomics; Q78T54-1; -. [Q78T54-1]
DR TopDownProteomics; Q78T54-2; -. [Q78T54-2]
DR Antibodypedia; 534; 79 antibodies from 23 providers.
DR Ensembl; ENSMUST00000114576; ENSMUSP00000110223; ENSMUSG00000073131. [Q78T54-1]
DR GeneID; 67048; -.
DR KEGG; mmu:67048; -.
DR UCSC; uc009tkf.3; mouse. [Q78T54-1]
DR CTD; 203547; -.
DR MGI; MGI:1914298; Vma21.
DR VEuPathDB; HostDB:ENSMUSG00000073131; -.
DR eggNOG; KOG4783; Eukaryota.
DR GeneTree; ENSGT00390000017980; -.
DR HOGENOM; CLU_143588_1_0_1; -.
DR InParanoid; Q78T54; -.
DR OMA; PYFRGNE; -.
DR OrthoDB; 1603333at2759; -.
DR TreeFam; TF314021; -.
DR BioGRID-ORCS; 67048; 12 hits in 51 CRISPR screens.
DR ChiTaRS; Vma21; mouse.
DR PRO; PR:Q78T54; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q78T54; protein.
DR Bgee; ENSMUSG00000073131; Expressed in otolith organ and 218 other tissues.
DR ExpressionAtlas; Q78T54; baseline and differential.
DR Genevisible; Q78T54; MM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005764; C:lysosome; ISO:MGI.
DR GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IBA:GO_Central.
DR HAMAP; MF_03058; VMA21; 1.
DR InterPro; IPR019013; Vma21.
DR PANTHER; PTHR31792; PTHR31792; 1.
DR Pfam; PF09446; VMA21; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..101
FT /note="Vacuolar ATPase assembly integral membrane protein
FT Vma21"
FT /id="PRO_0000331500"
FT TOPO_DOM 1..25
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT TOPO_DOM 47..65
FT /note="Lumenal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT TOPO_DOM 87..101
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT VAR_SEQ 55..101
FT /note="GALGMSNRDSYFYAAIVAVVAVHVVLALFVYVAWNEGSRQWREGKQD -> A
FT LRTKASLIDTLQLSSGRKKISPFRALQL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_037464"
SQ SEQUENCE 101 AA; 11365 MW; 5BDB68CC836190E1 CRC64;
MERLDKAALN ALQPPEFRNE NSLAATLKTL LFFTALMITV PIGLYFTTKA YIFEGALGMS
NRDSYFYAAI VAVVAVHVVL ALFVYVAWNE GSRQWREGKQ D
