ID   VMA21_NEUCR             Reviewed;         118 AA.
AC   Q7S158;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Vacuolar ATPase assembly integral membrane protein vma-21 {ECO:0000255|HAMAP-Rule:MF_03058};
GN   Name=vma-21; ORFNames=NCU09107;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Required for the assembly of the V0 complex of the vacuolar
CC       ATPase (V-ATPase) in the endoplasmic reticulum. {ECO:0000255|HAMAP-
CC       Rule:MF_03058}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000255|HAMAP-Rule:MF_03058}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_03058}. Endoplasmic reticulum-Golgi
CC       intermediate compartment membrane {ECO:0000255|HAMAP-Rule:MF_03058};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_03058}.
CC       Cytoplasmic vesicle, COPII-coated vesicle membrane {ECO:0000255|HAMAP-
CC       Rule:MF_03058}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_03058}.
CC   -!- SIMILARITY: Belongs to the VMA21 family. {ECO:0000255|HAMAP-
CC       Rule:MF_03058}.
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DR   EMBL; CM002236; EAA29080.1; -; Genomic_DNA.
DR   RefSeq; XP_958316.1; XM_953223.2.
DR   AlphaFoldDB; Q7S158; -.
DR   STRING; 5141.EFNCRP00000006484; -.
DR   EnsemblFungi; EAA29080; EAA29080; NCU09107.
DR   GeneID; 3874464; -.
DR   KEGG; ncr:NCU09107; -.
DR   VEuPathDB; FungiDB:NCU09107; -.
DR   HOGENOM; CLU_154717_1_1_1; -.
DR   InParanoid; Q7S158; -.
DR   OMA; AMKEDQT; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IBA:GO_Central.
DR   HAMAP; MF_03058; VMA21; 1.
DR   InterPro; IPR019013; Vma21.
DR   PANTHER; PTHR31792; PTHR31792; 1.
DR   Pfam; PF09446; VMA21; 1.
PE   3: Inferred from homology;
KW   Cytoplasmic vesicle; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..118
FT                   /note="Vacuolar ATPase assembly integral membrane protein
FT                   vma-21"
FT                   /id="PRO_0000377592"
FT   TOPO_DOM        1..35
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TOPO_DOM        57..73
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TRANSMEM        74..94
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   TOPO_DOM        95..118
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03058"
FT   REGION          98..118
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           115..118
FT                   /note="Prevents secretion from ER"
SQ   SEQUENCE   118 AA;  12566 MW;  63E61E10F9F65972 CRC64;
     MATRRIISQE KTLLEKDDRI GSSPAASEKS NITPAVPASV IIKLLAFTFA MIVIPISSYF
     LTVDRLFKGN STYAGATAAI MANVVLIGYI IVAMAEDQSD QENEKKGGGG KGEGKKDL