VMA22_YEAST
ID VMA22_YEAST Reviewed; 181 AA.
AC P38784; D3DL09;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Vacuolar ATPase assembly protein VMA22;
GN Name=VMA22; OrderedLocusNames=YHR060W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7673216; DOI=10.1074/jbc.270.38.22329;
RA Hill K.J., Stevens T.H.;
RT "Vma22p is a novel endoplasmic reticulum-associated protein required for
RT assembly of the yeast vacuolar H(+)-ATPase complex.";
RL J. Biol. Chem. 270:22329-22336(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2 (ISOFORM 2), CLEAVAGE OF
RP INITIATOR METHIONINE [LARGE SCALE ANALYSIS] (ISOFORM 2), AND IDENTIFICATION
RP BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Required for V-ATPase activity.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=1;
CC IsoId=P38784-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P38784-2; Sequence=VSP_058123;
CC -!- MISCELLANEOUS: Present with 752 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U24501; AAA87458.1; -; Genomic_DNA.
DR EMBL; U00061; AAB68380.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06753.1; -; Genomic_DNA.
DR PIR; S46701; S46701.
DR RefSeq; NP_011927.1; NM_001179190.1. [P38784-1]
DR AlphaFoldDB; P38784; -.
DR BioGRID; 36492; 117.
DR DIP; DIP-1736N; -.
DR IntAct; P38784; 10.
DR MINT; P38784; -.
DR STRING; 4932.YHR060W; -.
DR MaxQB; P38784; -.
DR PaxDb; P38784; -.
DR PRIDE; P38784; -.
DR EnsemblFungi; YHR060W_mRNA; YHR060W; YHR060W. [P38784-1]
DR GeneID; 856457; -.
DR KEGG; sce:YHR060W; -.
DR SGD; S000001102; VMA22.
DR VEuPathDB; FungiDB:YHR060W; -.
DR eggNOG; ENOG502S50X; Eukaryota.
DR HOGENOM; CLU_089394_0_0_1; -.
DR InParanoid; P38784; -.
DR OMA; RANYHNK; -.
DR BioCyc; YEAST:G3O-31113-MON; -.
DR PRO; PR:P38784; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38784; protein.
DR GO; GO:0042406; C:extrinsic component of endoplasmic reticulum membrane; IDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:1990871; C:Vma12-Vma22 assembly complex; IPI:SGD.
DR GO; GO:0051082; F:unfolded protein binding; IMP:SGD.
DR GO; GO:0007035; P:vacuolar acidification; IMP:SGD.
DR GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IMP:SGD.
DR InterPro; IPR040357; Vma22/CCDC115.
DR PANTHER; PTHR31996; PTHR31996; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative initiation; Reference proteome.
FT CHAIN 1..181
FT /note="Vacuolar ATPase assembly protein VMA22"
FT /id="PRO_0000065876"
FT REGION 93..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..5
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_058123"
FT INIT_MET P38784-2:1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES P38784-2:2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22814378"
SQ SEQUENCE 181 AA; 21078 MW; C1CB5DC8292D2734 CRC64;
MSETRMAQNM DTTDEQYLRL IELLSNYDST LEQLQKGFQD GYIQLSRSNY YNKDSLRGNY
GEDYWDETYI GQLMATVEEK NSKVVVEIVK RKAQDKQEKK EEEDNKLTQR KKGTKPEKQK
TQSHKLKQDY DPILMFGGVL SVPSSLRQSQ TSFKGCIPLI AQLINYKNEI LTLVETLSEQ
E
