VMAT2_HUMAN
ID VMAT2_HUMAN Reviewed; 514 AA.
AC Q05940; B2RC96; D3DRC4; Q15876; Q4G147; Q5VW49; Q9H3P6;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 193.
DE RecName: Full=Synaptic vesicular amine transporter;
DE AltName: Full=Monoamine transporter;
DE AltName: Full=Solute carrier family 18 member 2;
DE AltName: Full=Vesicular amine transporter 2;
DE Short=VAT2;
GN Name=SLC18A2; Synonyms=SVMT, VMAT2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=8095030; DOI=10.1016/0014-5793(93)80539-7;
RA Surratt C.K., Persico A.M., Yang X.D., Edgar S.R., Bird G.S., Hawkins A.L.,
RA Griffin C.A., Li X., Jabs E.W., Uhl G.R.;
RT "A human synaptic vesicle monoamine transporter cDNA predicts
RT posttranslational modifications, reveals chromosome 10 gene localization
RT and identifies TaqI RFLPs.";
RL FEBS Lett. 318:325-330(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=8245983; DOI=10.1111/j.1471-4159.1993.tb07476.x;
RA Erickson J.D., Eiden L.E.;
RT "Functional identification and molecular cloning of a human brain vesicle
RT monoamine transporter.";
RL J. Neurochem. 61:2314-2317(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Substantia nigra;
RX PubMed=7905859; DOI=10.1016/s0888-7543(05)80383-0;
RA Peter D., Finn J.P., Klisak I., Liu Y., Kojis T., Heinzmann C., Roghani A.,
RA Sparkes R.S., Edwards R.H.;
RT "Chromosomal localization of the human vesicular amine transporter genes.";
RL Genomics 18:720-723(1993).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=8373557; DOI=10.1007/bf01244941;
RA Lesch K.P., Gross J., Wolozin B.L., Murphy D.L., Riederer P.;
RT "Extensive sequence divergence between the human and rat brain vesicular
RT monoamine transporter: possible molecular basis for species differences in
RT the susceptibility to MPP+.";
RL J. Neural Transm. 93:75-82(1993).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11443533; DOI=10.1038/sj.mp.4000895;
RA Kunugi H., Ishida S., Akahane A., Nanko S.;
RT "Exon/intron boundaries, novel polymorphisms, and association analysis with
RT schizophrenia of the human synaptic vesicle monoamine transporter (SVMT)
RT gene.";
RL Mol. Psychiatry 6:456-460(2001).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Uterus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP DISULFIDE BOND.
RX PubMed=12009896; DOI=10.1021/bi015779j;
RA Thiriot D.S., Sievert M.K., Ruoho A.E.;
RT "Identification of human vesicle monoamine transporter (VMAT2) lumenal
RT cysteines that form an intramolecular disulfide bond.";
RL Biochemistry 41:6346-6353(2002).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Platelet;
RX PubMed=18088087; DOI=10.1021/pr0704130;
RA Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J.,
RA Schuetz C., Walter U., Gambaryan S., Sickmann A.;
RT "Phosphoproteome of resting human platelets.";
RL J. Proteome Res. 7:526-534(2008).
RN [12]
RP INVOLVEMENT IN PKDYS2, VARIANT PKDYS2 LEU-387, FUNCTION, AND
RP CHARACTERIZATION OF VARIANT PKDYS2 LEU-387.
RX PubMed=23363473; DOI=10.1056/nejmoa1207281;
RA Rilstone J.J., Alkhater R.A., Minassian B.A.;
RT "Brain dopamine-serotonin vesicular transport disease and its treatment.";
RL N. Engl. J. Med. 368:543-550(2013).
CC -!- FUNCTION: Involved in the ATP-dependent vesicular transport of biogenic
CC amine neurotransmitters. Pumps cytosolic monoamines including dopamine,
CC norepinephrine, serotonin, and histamine into synaptic vesicles
CC (PubMed:23363473). Requisite for vesicular amine storage prior to
CC secretion via exocytosis. {ECO:0000269|PubMed:23363473}.
CC -!- SUBUNIT: Interacts with SLC6A3. {ECO:0000250}.
CC -!- INTERACTION:
CC Q05940; Q6PL45-2: BRICD5; NbExp=3; IntAct=EBI-18036244, EBI-12244618;
CC Q05940; Q9BXR6: CFHR5; NbExp=3; IntAct=EBI-18036244, EBI-11579371;
CC Q05940; Q96DZ9-2: CMTM5; NbExp=3; IntAct=EBI-18036244, EBI-11522780;
CC Q05940; P25025: CXCR2; NbExp=3; IntAct=EBI-18036244, EBI-2835281;
CC Q05940; Q8TDV0: GPR151; NbExp=3; IntAct=EBI-18036244, EBI-11955647;
CC Q05940; Q6N075: MFSD5; NbExp=3; IntAct=EBI-18036244, EBI-3920969;
CC Q05940; Q16617: NKG7; NbExp=3; IntAct=EBI-18036244, EBI-3919611;
CC Q05940; P30825: SLC7A1; NbExp=3; IntAct=EBI-18036244, EBI-4289564;
CC Q05940; Q9BVC6: TMEM109; NbExp=3; IntAct=EBI-18036244, EBI-1057733;
CC Q05940; Q96B49: TOMM6; NbExp=3; IntAct=EBI-18036244, EBI-10826510;
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane
CC protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q05940-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q05940-2; Sequence=VSP_057151, VSP_057152;
CC -!- DISEASE: Parkinsonism-dystonia 2, infantile-onset (PKDYS2)
CC [MIM:618049]: An autosomal recessive disorder characterized by
CC infantile onset of abnormal movements, including parkinsonism,
CC dystonia, and poor fine motor skills, as well as autonomic dysfunction,
CC including abnormal sweating, cold extremities, and poor sleep. Some
CC patients have variable degrees of developmental delay.
CC {ECO:0000269|PubMed:23363473}. Note=The disease is caused by variants
CC affecting the gene represented in this entry.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Vesicular
CC transporter family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB19009.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Wikipedia; Note=Vesicular monoamine transporter
CC entry;
CC URL="https://en.wikipedia.org/wiki/Vesicular_monoamine_transporter";
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DR EMBL; L09118; AAA59877.1; -; mRNA.
DR EMBL; L23205; AAA61290.1; -; mRNA.
DR EMBL; L14269; AAA91853.1; -; mRNA.
DR EMBL; X71354; CAA50489.1; -; mRNA.
DR EMBL; AB044401; BAB19009.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK314997; BAG37493.1; -; mRNA.
DR EMBL; AL391988; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL731557; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471066; EAW49428.1; -; Genomic_DNA.
DR EMBL; CH471066; EAW49429.1; -; Genomic_DNA.
DR EMBL; BC030593; AAH30593.1; -; mRNA.
DR EMBL; BC108927; AAI08928.1; -; mRNA.
DR EMBL; BC108928; AAI08929.1; -; mRNA.
DR CCDS; CCDS7599.1; -. [Q05940-1]
DR PIR; A49368; A49368.
DR PIR; S29810; S29810.
DR RefSeq; NP_003045.2; NM_003054.4. [Q05940-1]
DR AlphaFoldDB; Q05940; -.
DR SMR; Q05940; -.
DR BioGRID; 112459; 104.
DR IntAct; Q05940; 10.
DR STRING; 9606.ENSP00000298472; -.
DR BindingDB; Q05940; -.
DR ChEMBL; CHEMBL1893; -.
DR DrugBank; DB01472; 4-Methoxyamphetamine.
DR DrugBank; DB00182; Amphetamine.
DR DrugBank; DB00714; Apomorphine.
DR DrugBank; DB00865; Benzphetamine.
DR DrugBank; DB01089; Deserpidine.
DR DrugBank; DB12161; Deutetrabenazine.
DR DrugBank; DB01576; Dextroamphetamine.
DR DrugBank; DB00988; Dopamine.
DR DrugBank; DB01363; Ephedra sinica root.
DR DrugBank; DB01364; Ephedrine.
DR DrugBank; DB00502; Haloperidol.
DR DrugBank; DB05381; Histamine.
DR DrugBank; DB06706; Isometheptene.
DR DrugBank; DB00579; Mazindol.
DR DrugBank; DB01577; Metamfetamine.
DR DrugBank; DB01454; Midomafetamine.
DR DrugBank; DB01442; MMDA.
DR DrugBank; DB04821; Nomifensine.
DR DrugBank; DB00368; Norepinephrine.
DR DrugBank; DB06714; Propylhexedrine.
DR DrugBank; DB09363; Rauwolfia serpentina root.
DR DrugBank; DB00206; Reserpine.
DR DrugBank; DB08839; Serotonin.
DR DrugBank; DB04844; Tetrabenazine.
DR DrugCentral; Q05940; -.
DR GuidetoPHARMACOLOGY; 1012; -.
DR TCDB; 2.A.1.2.29; the major facilitator superfamily (mfs).
DR GlyGen; Q05940; 2 sites.
DR iPTMnet; Q05940; -.
DR PhosphoSitePlus; Q05940; -.
DR BioMuta; SLC18A2; -.
DR DMDM; 1722742; -.
DR EPD; Q05940; -.
DR jPOST; Q05940; -.
DR MassIVE; Q05940; -.
DR PaxDb; Q05940; -.
DR PeptideAtlas; Q05940; -.
DR PRIDE; Q05940; -.
DR ProteomicsDB; 58362; -. [Q05940-1]
DR ABCD; Q05940; 1 sequenced antibody.
DR Antibodypedia; 2802; 394 antibodies from 38 providers.
DR DNASU; 6571; -.
DR Ensembl; ENST00000644641.2; ENSP00000496339.1; ENSG00000165646.14. [Q05940-1]
DR GeneID; 6571; -.
DR KEGG; hsa:6571; -.
DR MANE-Select; ENST00000644641.2; ENSP00000496339.1; NM_003054.6; NP_003045.2.
DR UCSC; uc001ldd.3; human. [Q05940-1]
DR CTD; 6571; -.
DR DisGeNET; 6571; -.
DR GeneCards; SLC18A2; -.
DR HGNC; HGNC:10935; SLC18A2.
DR HPA; ENSG00000165646; Tissue enhanced (brain, cervix).
DR MalaCards; SLC18A2; -.
DR MIM; 193001; gene.
DR MIM; 618049; phenotype.
DR neXtProt; NX_Q05940; -.
DR OpenTargets; ENSG00000165646; -.
DR Orphanet; 352649; Brain dopamine-serotonin vesicular transport disease.
DR PharmGKB; PA325; -.
DR VEuPathDB; HostDB:ENSG00000165646; -.
DR eggNOG; KOG3764; Eukaryota.
DR GeneTree; ENSGT00940000157593; -.
DR HOGENOM; CLU_001265_10_9_1; -.
DR InParanoid; Q05940; -.
DR OMA; IVAPLWG; -.
DR OrthoDB; 956763at2759; -.
DR PhylomeDB; Q05940; -.
DR TreeFam; TF313494; -.
DR PathwayCommons; Q05940; -.
DR Reactome; R-HSA-181429; Serotonin Neurotransmitter Release Cycle.
DR Reactome; R-HSA-181430; Norepinephrine Neurotransmitter Release Cycle.
DR Reactome; R-HSA-212676; Dopamine Neurotransmitter Release Cycle.
DR Reactome; R-HSA-442660; Na+/Cl- dependent neurotransmitter transporters.
DR SignaLink; Q05940; -.
DR SIGNOR; Q05940; -.
DR BioGRID-ORCS; 6571; 9 hits in 1077 CRISPR screens.
DR ChiTaRS; SLC18A2; human.
DR GeneWiki; Vesicular_monoamine_transporter_2; -.
DR GenomeRNAi; 6571; -.
DR Pharos; Q05940; Tclin.
DR PRO; PR:Q05940; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q05940; protein.
DR Bgee; ENSG00000165646; Expressed in substantia nigra pars reticulata and 121 other tissues.
DR Genevisible; Q05940; HS.
DR GO; GO:0005813; C:centrosome; IDA:HPA.
DR GO; GO:0070083; C:clathrin-sculpted monoamine transport vesicle membrane; TAS:Reactome.
DR GO; GO:0098691; C:dopaminergic synapse; IEA:Ensembl.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0016020; C:membrane; TAS:ProtInc.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0008021; C:synaptic vesicle; TAS:ParkinsonsUK-UCL.
DR GO; GO:0030672; C:synaptic vesicle membrane; IBA:GO_Central.
DR GO; GO:0043195; C:terminal bouton; IBA:GO_Central.
DR GO; GO:0008504; F:monoamine transmembrane transporter activity; TAS:Reactome.
DR GO; GO:0005335; F:serotonin:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015842; P:aminergic neurotransmitter loading into synaptic vesicle; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; TAS:ParkinsonsUK-UCL.
DR GO; GO:0015872; P:dopamine transport; TAS:ParkinsonsUK-UCL.
DR GO; GO:0007626; P:locomotory behavior; IEA:Ensembl.
DR GO; GO:0015844; P:monoamine transport; TAS:ProtInc.
DR GO; GO:1903427; P:negative regulation of reactive oxygen species biosynthetic process; NAS:ParkinsonsUK-UCL.
DR GO; GO:0098700; P:neurotransmitter loading into synaptic vesicle; TAS:ParkinsonsUK-UCL.
DR GO; GO:0006836; P:neurotransmitter transport; TAS:Reactome.
DR GO; GO:0009791; P:post-embryonic development; IEA:Ensembl.
DR GO; GO:0001975; P:response to amphetamine; IEA:Ensembl.
DR GO; GO:0009636; P:response to toxic substance; IEA:Ensembl.
DR GO; GO:0042137; P:sequestering of neurotransmitter; NAS:ParkinsonsUK-UCL.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; Disease variant; Disulfide bond;
KW Dystonia; Glycoprotein; Membrane; Neurotransmitter transport; Parkinsonism;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..514
FT /note="Synaptic vesicular amine transporter"
FT /id="PRO_0000127514"
FT TOPO_DOM 1..20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 21..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..129
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..159
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..189
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..248
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..291
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..328
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..357
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 379..389
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 411..414
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 436..440
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 441..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..514
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 511
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q01827"
FT MOD_RES 513
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q01827"
FT CARBOHYD 84
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 91
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 117..324
FT /evidence="ECO:0000269|PubMed:12009896"
FT VAR_SEQ 204..209
FT /note="MGMLAS -> RCGMPE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_057151"
FT VAR_SEQ 210..514
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_057152"
FT VARIANT 387
FT /note="P -> L (in PKDYS2; strong decrease in serotonin
FT transport; dbSNP:rs1392638187)"
FT /evidence="ECO:0000269|PubMed:23363473"
FT /id="VAR_081069"
FT CONFLICT 17
FT /note="R -> H (in Ref. 4; CAA50489)"
FT /evidence="ECO:0000305"
FT CONFLICT 302
FT /note="C -> S (in Ref. 1; AAA59877)"
FT /evidence="ECO:0000305"
FT CONFLICT 354
FT /note="K -> T (in Ref. 1; AAA59877)"
FT /evidence="ECO:0000305"
FT CONFLICT 378
FT /note="A -> P (in Ref. 1; AAA59877)"
FT /evidence="ECO:0000305"
FT CONFLICT 395
FT /note="I -> N (in Ref. 1; AAA59877)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 514 AA; 55713 MW; 42EA91A225485DB2 CRC64;
MALSELALVR WLQESRRSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS IKHEKNATEI
QTARPVHTAS ISDSFQSIFS YYDNSTMVTG NATRDLTLHQ TATQHMVTNA SAVPSDCPSE
DKDLLNENVQ VGLLFASKAT VQLITNPFIG LLTNRIGYPI PIFAGFCIMF VSTIMFAFSS
SYAFLLIARS LQGIGSSCSS VAGMGMLASV YTDDEERGNV MGIALGGLAM GVLVGPPFGS
VLYEFVGKTA PFLVLAALVL LDGAIQLFVL QPSRVQPESQ KGTPLTTLLK DPYILIAAGS
ICFANMGIAM LEPALPIWMM ETMCSRKWQL GVAFLPASIS YLIGTNIFGI LAHKMGRWLC
ALLGMIIVGV SILCIPFAKN IYGLIAPNFG VGFAIGMVDS SMMPIMGYLV DLRHVSVYGS
VYAIADVAFC MGYAIGPSAG GAIAKAIGFP WLMTIIGIID ILFAPLCFFL RSPPAKEEKM
AILMDHNCPI KTKMYTQNNI QSYPIGEDEE SESD
