ID   VME1_BCHK3              Reviewed;         221 AA.
AC   Q3LZX9;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Membrane protein {ECO:0000255|HAMAP-Rule:MF_04202};
DE            Short=M protein {ECO:0000255|HAMAP-Rule:MF_04202};
DE   AltName: Full=E1 glycoprotein {ECO:0000255|HAMAP-Rule:MF_04202};
DE   AltName: Full=Matrix glycoprotein {ECO:0000255|HAMAP-Rule:MF_04202};
DE   AltName: Full=Membrane glycoprotein {ECO:0000255|HAMAP-Rule:MF_04202};
GN   Name=M {ECO:0000255|HAMAP-Rule:MF_04202}; ORFNames=5;
OS   Bat coronavirus HKU3 (BtCoV) (SARS-like coronavirus HKU3).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Sarbecovirus.
OX   NCBI_TaxID=442736;
OH   NCBI_TaxID=89399; Rhinolophus sinicus (Chinese rufous horseshoe bat).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate HKU3-1;
RX   PubMed=16169905; DOI=10.1073/pnas.0506735102;
RA   Lau S.K.P., Woo P.C.Y., Li K.S.M., Huang Y., Tsoi H.-W., Wong B.H.L.,
RA   Wong S.S.Y., Leung S.-Y., Chan K.-H., Yuen K.-Y.;
RT   "Severe acute respiratory syndrome coronavirus-like virus in Chinese
RT   horseshoe bats.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:14040-14045(2005).
CC   -!- FUNCTION: Component of the viral envelope that plays a central role in
CC       virus morphogenesis and assembly via its interactions with other viral
CC       proteins. {ECO:0000255|HAMAP-Rule:MF_04202, ECO:0000255|PROSITE-
CC       ProRule:PRU01275}.
CC   -!- SUBUNIT: Homomultimer. Interacts with envelope E protein in the budding
CC       compartment of the host cell, which is located between endoplasmic
CC       reticulum and the Golgi complex. Forms a complex with HE and S
CC       proteins. Interacts with nucleocapsid N protein. This interaction
CC       probably participates in RNA packaging into the virus.
CC       {ECO:0000255|HAMAP-Rule:MF_04202, ECO:0000255|PROSITE-
CC       ProRule:PRU01275}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000255|HAMAP-
CC       Rule:MF_04202}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_04202}. Host Golgi apparatus membrane {ECO:0000255|HAMAP-
CC       Rule:MF_04202}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_04202}. Note=Largely embedded in the lipid bilayer.
CC       {ECO:0000255|HAMAP-Rule:MF_04202}.
CC   -!- MISCELLANEOUS: Bat coronavirus HKU3 is highly similar to SARS-CoV
CC       (SARS-like).
CC   -!- SIMILARITY: Belongs to the betacoronaviruses M protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04202}.
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DR   EMBL; DQ022305; AAY88869.1; -; Genomic_RNA.
DR   Proteomes; UP000007450; Genome.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-UniRule.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039660; F:structural constituent of virion; IEA:UniProtKB-UniRule.
DR   CDD; cd21569; SARS-like-CoV_M; 1.
DR   HAMAP; MF_04202; BETA_CORONA_M; 1.
DR   InterPro; IPR002574; M_CoV.
DR   InterPro; IPR044361; M_SARS-like-CoV.
DR   Pfam; PF01635; CoV_M; 1.
DR   PROSITE; PS51927; COV_M; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Host Golgi apparatus; Host membrane; Host-virus interaction;
KW   Membrane; Transmembrane; Transmembrane helix; Viral envelope protein;
KW   Viral immunoevasion; Viral matrix protein; Virion.
FT   CHAIN           1..221
FT                   /note="Membrane protein"
FT                   /id="PRO_0000291372"
FT   TOPO_DOM        1..18
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT   TOPO_DOM        40..49
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT   TRANSMEM        50..70
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT   TOPO_DOM        71..78
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT   TOPO_DOM        100..221
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
SQ   SEQUENCE   221 AA;  25091 MW;  FBF6C7E7466A8AE1 CRC64;
     MADNGTITVE ELKQLLEQWN LVIGFIFLAW IMLLQFAYSN RNRFLYIIKL VFLWLLWPVT
     LACFVLAAVY RINWVTGGIA IAMACIVGLM WLSYFVASFR LFARTRSMWS FNPETNILLN
     VPLRGTILTR PLMESELVIG AVIIRGHLRM AGHSLGRCDI KDLPKEITVA TSRTLSYYKL
     GASQRVGTDS GFAAYNRYRI GNYKLNTDHS GSNDNIALLV Q