VME1_CVMJH
ID VME1_CVMJH Reviewed; 228 AA.
AC P08549;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Membrane protein {ECO:0000255|HAMAP-Rule:MF_04202};
DE Short=M protein {ECO:0000255|HAMAP-Rule:MF_04202};
DE AltName: Full=E1 glycoprotein {ECO:0000255|HAMAP-Rule:MF_04202};
DE AltName: Full=Matrix glycoprotein {ECO:0000255|HAMAP-Rule:MF_04202};
DE AltName: Full=Membrane glycoprotein {ECO:0000255|HAMAP-Rule:MF_04202};
GN Name=M {ECO:0000255|HAMAP-Rule:MF_04202}; ORFNames=6;
OS Murine coronavirus (strain JHM) (MHV-JHM) (Murine hepatitis virus).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Betacoronavirus; Embecovirus.
OX NCBI_TaxID=11144;
OH NCBI_TaxID=10090; Mus musculus (Mouse).
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3748812; DOI=10.1093/nar/14.15.6338;
RA Pfleiderer M., Skinner M.A., Siddell S.G.;
RT "Coronavirus MHV-JHM: nucleotide sequence of the mRNA that encodes the
RT membrane protein.";
RL Nucleic Acids Res. 14:6338-6338(1986).
RN [2]
RP INTERACTION WITH E PROTEIN.
RX PubMed=10544100; DOI=10.1006/viro.1999.9955;
RA Maeda J., Maeda A., Makino S.;
RT "Release of coronavirus E protein in membrane vesicles from virus-infected
RT cells and E protein-expressing cells.";
RL Virology 263:265-272(1999).
RN [3]
RP FUNCTION, AND INTERACTION WITH N PROTEIN.
RX PubMed=10933723; DOI=10.1128/jvi.74.17.8127-8134.2000;
RA Narayanan K., Maeda A., Maeda J., Makino S.;
RT "Characterization of the coronavirus M protein and nucleocapsid interaction
RT in infected cells.";
RL J. Virol. 74:8127-8134(2000).
CC -!- FUNCTION: Component of the viral envelope that plays a central role in
CC virus morphogenesis and assembly via its interactions with other viral
CC proteins. {ECO:0000255|HAMAP-Rule:MF_04202, ECO:0000255|PROSITE-
CC ProRule:PRU01275, ECO:0000269|PubMed:10933723}.
CC -!- SUBUNIT: Homomultimer. Interacts with envelope E protein in the budding
CC compartment of the host cell, which is located between endoplasmic
CC reticulum and the Golgi complex. Forms a complex with HE and S
CC proteins. Interacts with nucleocapsid N protein. This interaction
CC probably participates in RNA packaging into the virus.
CC {ECO:0000255|HAMAP-Rule:MF_04202, ECO:0000255|PROSITE-
CC ProRule:PRU01275}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000255|HAMAP-
CC Rule:MF_04202}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_04202}. Host Golgi apparatus membrane {ECO:0000255|HAMAP-
CC Rule:MF_04202}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_04202}. Note=Largely embedded in the lipid bilayer.
CC {ECO:0000255|HAMAP-Rule:MF_04202}.
CC -!- SIMILARITY: Belongs to the betacoronaviruses M protein family.
CC {ECO:0000255|HAMAP-Rule:MF_04202}.
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DR EMBL; X04223; CAA27802.1; -; mRNA.
DR PIR; A25304; VGIHJH.
DR RefSeq; YP_209237.1; AC_000192.1.
DR SMR; P08549; -.
DR KEGG; vg:3283261; -.
DR Proteomes; UP000007193; Genome.
DR GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-UniRule.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0039660; F:structural constituent of virion; IEA:UniProtKB-UniRule.
DR CDD; cd21568; HCoV-like_M; 1.
DR HAMAP; MF_04202; BETA_CORONA_M; 1.
DR InterPro; IPR002574; M_CoV.
DR InterPro; IPR044362; M_HCoV-like.
DR Pfam; PF01635; CoV_M; 1.
DR PROSITE; PS51927; COV_M; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Host Golgi apparatus; Host membrane; Host-virus interaction;
KW Membrane; Transmembrane; Transmembrane helix; Viral envelope protein;
KW Viral immunoevasion; Viral matrix protein; Virion.
FT CHAIN 1..228
FT /note="Membrane protein"
FT /id="PRO_0000106038"
FT TOPO_DOM 2..25
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT TOPO_DOM 47..56
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT TOPO_DOM 78..85
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
FT TOPO_DOM 107..228
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04202"
SQ SEQUENCE 228 AA; 26051 MW; 82009BB56AE63038 CRC64;
MSSTTQAPGP VYQWTADEAV QFLKEWNFSL GIILLFITII LQFGYTSRSM FIYVVKMIIL
WLMWPLIIVL CMFNCVYALN NVYLGFSIVF TIVSVVMWIM YFVNSIRLFI RTGSWWSFNP
ETNNLMCIDM KGTVYVRPII EDYHTLTATI IRGHFYMQGV KLGTGFSLSD LPAYVTVAKV
SHLCTYKRAF LDKVDGVSGF AVYVKSKVGN YRLPSNKPSG ADTVLLRI
