ID   VME1_CVPFS              Reviewed;         262 AA.
AC   P09175;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   23-FEB-2022, entry version 91.
DE   RecName: Full=Membrane protein {ECO:0000255|HAMAP-Rule:MF_04201};
DE            Short=M protein {ECO:0000255|HAMAP-Rule:MF_04201};
DE   AltName: Full=E1 glycoprotein {ECO:0000255|HAMAP-Rule:MF_04201};
DE   AltName: Full=Matrix glycoprotein {ECO:0000255|HAMAP-Rule:MF_04201};
DE   AltName: Full=Membrane glycoprotein {ECO:0000255|HAMAP-Rule:MF_04201};
GN   Name=M {ECO:0000255|HAMAP-Rule:MF_04201}; ORFNames=5;
OS   Porcine transmissible gastroenteritis coronavirus (strain FS772/70) (TGEV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Tegacovirus.
OX   NCBI_TaxID=11150;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2845226; DOI=10.1111/j.1365-2958.1988.tb00056.x;
RA   Britton P., Carmenes R.S., Page K.W., Garwes D.J.;
RT   "The integral membrane protein from a virulent isolate of transmissible
RT   gastroenteritis virus: molecular characterization, sequence and expression
RT   in Escherichia coli.";
RL   Mol. Microbiol. 2:497-505(1988).
CC   -!- FUNCTION: Component of the viral envelope that plays a central role in
CC       virus morphogenesis and assembly via its interactions with other viral
CC       proteins. {ECO:0000255|HAMAP-Rule:MF_04201, ECO:0000255|PROSITE-
CC       ProRule:PRU01275}.
CC   -!- SUBUNIT: Homomultimer. Interacts with envelope E protein in the budding
CC       compartment of the host cell, which is located between endoplasmic
CC       reticulum and the Golgi complex. Forms a complex with HE and S
CC       proteins. Interacts with nucleocapsid N protein. This interaction
CC       probably participates in RNA packaging into the virus.
CC       {ECO:0000255|HAMAP-Rule:MF_04201, ECO:0000255|PROSITE-
CC       ProRule:PRU01275}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000255|HAMAP-
CC       Rule:MF_04201}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_04201}. Host Golgi apparatus membrane {ECO:0000255|HAMAP-
CC       Rule:MF_04201}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_04201}. Note=Largely embedded in the lipid bilayer.
CC       {ECO:0000255|HAMAP-Rule:MF_04201}.
CC   -!- SIMILARITY: Belongs to the alphacoronaviruses M protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04201}.
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DR   EMBL; Y00560; CAA68643.1; -; Genomic_RNA.
DR   PIR; S01914; S01914.
DR   SMR; P09175; -.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-UniRule.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039660; F:structural constituent of virion; IEA:UniProtKB-UniRule.
DR   CDD; cd21564; alphaCoV_M; 1.
DR   HAMAP; MF_04201; ALPHA_CORONA_M; 1.
DR   InterPro; IPR042551; ALPHA_CORONA_M.
DR   InterPro; IPR002574; M_CoV.
DR   Pfam; PF01635; CoV_M; 1.
DR   PROSITE; PS51927; COV_M; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Host Golgi apparatus; Host membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Viral envelope protein; Viral matrix protein; Virion.
FT   CHAIN           1..262
FT                   /note="Membrane protein"
FT                   /id="PRO_0000037154"
FT   TOPO_DOM        18..47
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT   TOPO_DOM        69..77
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT   TOPO_DOM        99..112
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT   TRANSMEM        113..133
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT   TOPO_DOM        134..262
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT   REGION          237..252
FT                   /note="Interaction with N protein"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04201"
FT   CARBOHYD        32
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
SQ   SEQUENCE   262 AA;  29459 MW;  79E3847C6C74671F CRC64;
     MKLLLILACV IACACGERYC AMKDDTGLSC RNSTASACES CFNGGDLIWH LANWNFSWSI
     ILIIFITVLQ YGRPQFSWFV YGIKMLIMWL LWPIVLALTI FNAYSEYQVS RYVMFGVSIA
     GAIVTFVLWI MYFVRSIQLY RRTKSWWSFN PEINAILCVS ALGRSYVLPL EGVPTGVTLT
     LLSGNLYAEG FKIAGGMNID NLPKYVMVAL PSRTIVYTLV GKKLKASSAT GWAYYVKSKA
     GDYSTDARTD NLSEQEKLLH MV