ID   VME1_CVRNJ              Reviewed;         222 AA.
AC   Q9WCD1;
DT   11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   07-APR-2021, entry version 73.
DE   RecName: Full=Membrane protein;
DE            Short=M protein;
DE   AltName: Full=E1 glycoprotein;
DE   AltName: Full=Matrix glycoprotein;
DE   AltName: Full=Membrane glycoprotein;
DE   Flags: Fragment;
GN   Name=M;
OS   Rat coronavirus (strain NJ) (RCV-NJ).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Embecovirus.
OX   NCBI_TaxID=231433;
OH   NCBI_TaxID=10116; Rattus norvegicus (Rat).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=10551451;
RA   Compton S.R., Vivas-Gonzalez B.E., Macy J.D.;
RT   "Reverse transcriptase polymerase chain reaction-based diagnosis and
RT   molecular characterization of a new rat coronavirus strain.";
RL   Lab. Anim. Sci. 49:506-513(1999).
CC   -!- FUNCTION: Component of the viral envelope that plays a central role in
CC       virus morphogenesis and assembly via its interactions with other viral
CC       proteins. {ECO:0000255|PROSITE-ProRule:PRU01275}.
CC   -!- SUBUNIT: Homomultimer. Interacts with envelope E protein in the budding
CC       compartment of the host cell, which is located between endoplasmic
CC       reticulum and the Golgi complex. Forms a complex with HE and S
CC       proteins. Interacts with nucleocapsid N protein. This interaction
CC       probably participates in RNA packaging into the virus.
CC       {ECO:0000255|PROSITE-ProRule:PRU01275}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}. Host Golgi apparatus membrane
CC       {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. Note=Largely
CC       embedded in the lipid bilayer. {ECO:0000250}.
CC   -!- PTM: O-linked glycans consist of Gal-GalNAc disaccharides which are
CC       modified with up to 2 sialic acid residues. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the coronaviruses M protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF088985; AAD33105.1; -; Genomic_RNA.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039660; F:structural constituent of virion; IEA:UniProtKB-KW.
DR   CDD; cd21568; HCoV-like_M; 1.
DR   InterPro; IPR002574; M_CoV.
DR   InterPro; IPR044362; M_HCoV-like.
DR   Pfam; PF01635; CoV_M; 1.
DR   PROSITE; PS51927; COV_M; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Host Golgi apparatus; Host membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Viral envelope protein; Viral matrix protein; Virion.
FT   CHAIN           <1..222
FT                   /note="Membrane protein"
FT                   /id="PRO_0000106043"
FT   TOPO_DOM        <1..19
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        41..43
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        65..75
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        76..96
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        97..222
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        9
FT                   /note="O-linked (GalNAc...) threonine; by host"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   222 AA;  25420 MW;  D9012C28C0ACC76E CRC64;
     APQTVYEWTA DVAVRFLKEW NFLLGIILLF ITIILQFGYT SRSMFIYVVK MIILWLMWPL
     TIVLCIFNCV YALNNVYLGF SIVFTIVSIV MWIMYFVNSI RLFIRTGSWW SFNPETNNLM
     CIDVKGTVYV RPIIEDYHTL TATNVRGHLY MQGVKLGTGF SLSDLPAYVT VAKVSHLCTY
     KRAFLDKVDG VSGFAVYVKS KVGNYRLPSN KPSGVDTALL RI