ID   VMXBN_BOTJA             Reviewed;          29 AA.
AC   P20416;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Snake venom metalloproteinase bothrolysin;
DE            Short=SVMP;
DE            EC=3.4.24.50;
DE   AltName: Full=Hemorrhagic metalloproteinase J;
DE   AltName: Full=Zinc metalloproteinase bothrolysin;
DE   Flags: Fragment;
OS   Bothrops jararaca (Jararaca) (Bothrops jajaraca).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Bothrops.
OX   NCBI_TaxID=8724;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Venom;
RX   PubMed=2781574; DOI=10.1016/0041-0101(89)90041-x;
RA   Tanizaki M.M., Zingali R.B., Kawazaki H., Imajoh S., Yamazaki S.,
RA   Suzuki K.;
RT   "Purification and some characteristics of a zinc metalloprotease from the
RT   venom of Bothrops jararaca (jararaca).";
RL   Toxicon 27:747-755(1989).
CC   -!- FUNCTION: Snake venom zinc metalloproteinase that impairs hemostasis in
CC       the envenomed animal.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Cleavage of 4-Gln-|-His-5, 9-Ser-|-His-10 and 14-Ala-|-Leu-15
CC         of insulin B chain and Pro-|-Phe of angiotensin I.; EC=3.4.24.50;
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- SIMILARITY: Belongs to the venom metalloproteinase (M12B) family.
CC       {ECO:0000305}.
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DR   PIR; A32414; A32414.
DR   AlphaFoldDB; P20416; -.
DR   MEROPS; M12.139; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Hemostasis impairing toxin; Hydrolase;
KW   Metal-binding; Metalloprotease; Protease; Secreted; Toxin; Zinc.
FT   CHAIN           1..>29
FT                   /note="Snake venom metalloproteinase bothrolysin"
FT                   /id="PRO_0000078200"
FT   DOMAIN          6..>29
FT                   /note="Peptidase M12B"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00276"
FT   BINDING         9
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   NON_TER         29
SQ   SEQUENCE   29 AA;  3433 MW;  C26FE9C5A1D3E3BE CRC64;
     TPEHQRYIEL FLVVDSGMFM KYNGNSDKI