ID   VNFA_AZOVI              Reviewed;         522 AA.
AC   P12627;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Nitrogen fixation protein VnfA;
GN   Name=vnfA;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2722750; DOI=10.1128/jb.171.6.3258-3267.1989;
RA   Joerger R.D., Jacobson M.R., Bishop P.E.;
RT   "Two nifA-like genes required for expression of alternative nitrogenases by
RT   Azotobacter vinelandii.";
RL   J. Bacteriol. 171:3258-3267(1989).
CC   -!- FUNCTION: Required for the expression of the V-dependent nitrogen
CC       fixation system in Azotobacter vinelandii. It is required for the
CC       regulation of nitrogenase 2 transcription. Interacts with sigma-54.
CC   -!- INTERACTION:
CC       P12627; P12627: vnfA; NbExp=2; IntAct=EBI-7020421, EBI-7020421;
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M26752; AAA82516.1; -; Genomic_DNA.
DR   PIR; D44514; D44514.
DR   AlphaFoldDB; P12627; -.
DR   SMR; P12627; -.
DR   MINT; P12627; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF01590; GAF; 1.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   1: Evidence at protein level;
KW   Activator; ATP-binding; DNA-binding; Nitrogen fixation; Nucleotide-binding;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..522
FT                   /note="Nitrogen fixation protein VnfA"
FT                   /id="PRO_0000081317"
FT   DOMAIN          35..177
FT                   /note="GAF"
FT   DOMAIN          210..439
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        493..512
FT                   /note="H-T-H motif"
FT   REGION          22..183
FT                   /note="A domain"
FT   BINDING         238..245
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         301..310
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   522 AA;  57630 MW;  35619FD910AF59A1 CRC64;
     MSSLPQYCEC GLGECRTDVL PLLYEMSQIA TESGDLSSII SILLRLMKRH MKVVRGMVTL
     YDRDSGSIVL HESFGLSPEE AGKGVYLLGE GIIGRVVETG QSIVVPCIRD EPAFLNRTGS
     RDRDSDDANL SFICVPILRG RQVMGTISAE RLYDNAELLK LDVEVLSILA TTTAQAVELY
     LVENVENVAL EAENRRLRSA LGERFKPANI IGNSKPMLEV YQLIERVVRT RTTVLILGES
     GVGKELVAGA IHYNSPAAKG PFVKFNCASL PESVIESELF GHEKGSFTGA IGLRKGRFEE
     AAGGTIFLDE VGEMSLTTQA KLLRVLQERS FERVGGNTTI HVDLRVIAAT NRNLAEMVAD
     GTFAEDLYYR LNVFPITIPP LRERGSDIIT LADHFVSRFS REMGIEVNRI STPRLNMLQS
     YQWPGNVREL ENVIERAMLL SEDGVIHGYH LPPSLQAPVV GDSEAPPDGL EARLGAIEYE
     LIVEALKLHH GNMTEAATHL GLTARVLGLR MGKYNLNYKD YR