ID   VNFD_AZOSA              Reviewed;         279 AA.
AC   O68954;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Nitrogenase vanadium-iron protein alpha chain;
DE            EC=1.18.6.1;
DE   AltName: Full=Dinitrogenase 2 subunit alpha;
DE   AltName: Full=Nitrogenase component I;
DE   Flags: Fragment;
GN   Name=vnfD;
OS   Azotobacter salinestris.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=69964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10420583; DOI=10.1139/w99-007;
RA   Loveless T.M., Bishop P.E.;
RT   "Identification of genes unique to Mo-independent nitrogenase systems in
RT   diverse diazotrophs.";
RL   Can. J. Microbiol. 45:312-317(1999).
CC   -!- FUNCTION: This vanadium-iron protein is part of the nitrogenase complex
CC       that catalyzes the key enzymatic reactions in nitrogen fixation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC         ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC         phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC         ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:456216; EC=1.18.6.1;
CC   -!- COFACTOR:
CC       Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143; Evidence={ECO:0000250};
CC       Note=Binds 1 [8Fe-7S] cluster per heterodimer. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=[7Fe-V-9S-C-homocitryl] cluster; Xref=ChEBI:CHEBI:60357;
CC         Evidence={ECO:0000250};
CC       Note=Binds 1 [7Fe-V-9S-C-homocitryl] cluster per subunit.
CC       {ECO:0000250};
CC   -!- SUBUNIT: Hexamer of two alpha, two beta, and two delta chains.
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: The structure of the 7Fe-V-9S-C-homocitryl cluster is
CC       assumed to be analogous to the 7Fe-Mo-9S-C-homocitryl cluster.
CC   -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR   EMBL; AF058784; AAC14341.1; -; Genomic_DNA.
DR   AlphaFoldDB; O68954; -.
DR   SMR; O68954; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   InterPro; IPR000510; Nase/OxRdtase_comp1.
DR   InterPro; IPR005974; Nase_asu.
DR   InterPro; IPR010143; Nase_comp1_asu.
DR   InterPro; IPR000318; Nase_comp1_CS.
DR   PANTHER; PTHR43457; PTHR43457; 1.
DR   Pfam; PF00148; Oxidored_nitro; 1.
DR   TIGRFAMs; TIGR01284; alt_nitrog_alph; 1.
DR   PROSITE; PS00699; NITROGENASE_1_1; 1.
DR   PROSITE; PS00090; NITROGENASE_1_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Iron; Iron-sulfur; Metal-binding; Nitrogen fixation;
KW   Nucleotide-binding; Oxidoreductase; Vanadium.
FT   CHAIN           <1..>279
FT                   /note="Nitrogenase vanadium-iron protein alpha chain"
FT                   /id="PRO_0000153057"
FT   BINDING         5
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with beta chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         31
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with beta chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         94
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with beta chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         213
FT                   /ligand="[7Fe-V-9S-C-homocitryl] cluster"
FT                   /ligand_id="ChEBI:CHEBI:60357"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
FT   NON_TER         279
SQ   SEQUENCE   279 AA;  31795 MW;  76618195F65A5E55 CRC64;
     SERGCAFCGA KLVIGGVLKD TIQMIHGPLG CAYDTWHTKR YPTDNGHFNM KYVWSTDMKE
     SHVVFGGEKR LEKSMHEAFD EMPDIKRMIV YTTCPTALIG DDIKAVAKKV MKDRPDVDVF
     TVECPGFSGV SQSKGHHVLN IGWINEKVET MEKEITSEYT MNFIGDFNIQ GDTQLLQTYW
     DRLGIQVVAH FTGNGTYDDL RCMHQAQLNV VNCARSSGYI ANELKKRYGI PRLDIDSWGF
     NYMAEGIRKI CAFFGIEEKG EALIAEEYAK WKPKLDWYK