ID   VNFK_AZOCH              Reviewed;         475 AA.
AC   P15334;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Nitrogenase vanadium-iron protein beta chain;
DE            EC=1.18.6.1;
DE   AltName: Full=Dinitrogenase 2 subunit beta;
DE   AltName: Full=Nitrogenase component I;
GN   Name=vnfK;
OS   Azotobacter chroococcum mcd 1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2388847; DOI=10.1093/nar/18.15.4616;
RA   Fallik E., Robson R.L.;
RT   "Completed sequence of the region encoding the structural genes for the
RT   vanadium nitrogenase of Azotobacter chroococcum.";
RL   Nucleic Acids Res. 18:4616-4616(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-11.
RX   PubMed=2743980; DOI=10.1002/j.1460-2075.1989.tb03495.x;
RA   Robson R.L., Woodley P.R., Pau R.N., Eady R.R.;
RT   "Structural genes for the vanadium nitrogenase from Azotobacter
RT   chroococcum.";
RL   EMBO J. 8:1217-1224(1989).
CC   -!- FUNCTION: This vanadium-iron protein is part of the nitrogenase complex
CC       that catalyzes the key enzymatic reactions in nitrogen fixation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC         ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC         phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC         ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:456216; EC=1.18.6.1;
CC   -!- COFACTOR:
CC       Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143; Evidence={ECO:0000250};
CC       Note=Binds 1 [8Fe-7S] cluster per heterodimer. {ECO:0000250};
CC   -!- SUBUNIT: Hexamer of two alpha, two beta, and two delta chains.
CC   -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR   EMBL; X51756; CAA36060.1; -; Genomic_DNA.
DR   EMBL; X15077; CAA33175.1; -; Genomic_DNA.
DR   PIR; S04115; S04115.
DR   AlphaFoldDB; P15334; -.
DR   SMR; P15334; -.
DR   BRENDA; 1.18.6.2; 617.
DR   GO; GO:0016613; C:vanadium-iron nitrogenase complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   InterPro; IPR000510; Nase/OxRdtase_comp1.
DR   InterPro; IPR000318; Nase_comp1_CS.
DR   InterPro; IPR014281; Nase_VnfK.
DR   Pfam; PF00148; Oxidored_nitro; 1.
DR   TIGRFAMs; TIGR02932; vnfK_nitrog; 1.
DR   PROSITE; PS00699; NITROGENASE_1_1; 1.
DR   PROSITE; PS00090; NITROGENASE_1_2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Direct protein sequencing; Iron; Iron-sulfur; Metal-binding;
KW   Nitrogen fixation; Nucleotide-binding; Oxidoreductase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2743980"
FT   CHAIN           2..475
FT                   /note="Nitrogenase vanadium-iron protein beta chain"
FT                   /id="PRO_0000153088"
FT   BINDING         31
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         56
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         115
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         153
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   475 AA;  52855 MW;  4F73D43FC656DB42 CRC64;
     MSNCELTVLK PAEVKLVKRE REGIINPMYD CQPAGAQYAG IGVKDCIPLV HGGQGCTMFV
     RLLFAQHFKE NFDVASTSLH EESAVFGGAK RVEEGVLVLA RRYPELRLIP IITTCSTEVI
     GDDIEGTINV CNRALAAEFP ERKIYLAPVH TPSFKGSHVT GYAECVKSMF KTITEVHGKG
     QPSGKLNVFP GWVNPGDVVL LKRYFKEMGV DATVFMDTED FDSPMLPNKS IETHGRTTVE
     DIADSANALA TLALARYEGA TTGEYLEKTF AVPNSLVNTP YGIKNTDDML RKIAEITGKE
     IPESLVREPR IAWIALADLA HMFFANKKVA IFGHPDLVLG LAQFCLEVEL EPVLLLIGDD
     QGSKYKKDPR LQELKDAAHF DMEIVHNADL WELEKRINDG LQLDLIMGHS KGRYVAIEAN
     IPMVRVGFPT FDRAGLYRKP SIGYQGAMEL GEMIANAMFA HMEYTRNKEW ILNTW