ID   VNFK_TRIV2              Reviewed;         463 AA.
AC   Q57302; Q3M5V4;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 2.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Nitrogenase vanadium-iron protein beta chain;
DE            EC=1.18.6.1;
DE   AltName: Full=Dinitrogenase 2 subunit beta;
DE   AltName: Full=Nitrogenase component I;
GN   Name=vnfK; OrderedLocusNames=Ava_4027;
OS   Trichormus variabilis (strain ATCC 29413 / PCC 7937) (Anabaena variabilis).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Trichormus.
OX   NCBI_TaxID=240292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8407800; DOI=10.1128/jb.175.19.6276-6286.1993;
RA   Thiel T.;
RT   "Characterization of genes for an alternative nitrogenase in the
RT   cyanobacterium Anabaena variabilis.";
RL   J. Bacteriol. 175:6276-6286(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29413 / PCC 7937;
RX   PubMed=25197444; DOI=10.4056/sigs.3899418;
RA   Thiel T., Pratte B.S., Zhong J., Goodwin L., Copeland A., Lucas S., Han C.,
RA   Pitluck S., Land M.L., Kyrpides N.C., Woyke T.;
RT   "Complete genome sequence of Anabaena variabilis ATCC 29413.";
RL   Stand. Genomic Sci. 9:562-573(2014).
CC   -!- FUNCTION: This vanadium-iron protein is part of the nitrogenase complex
CC       that catalyzes the key enzymatic reactions in nitrogen fixation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16
CC         ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16
CC         phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997,
CC         ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:456216; EC=1.18.6.1;
CC   -!- COFACTOR:
CC       Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143; Evidence={ECO:0000250};
CC       Note=Binds 1 [8Fe-7S] cluster per heterodimer. {ECO:0000250};
CC   -!- SUBUNIT: Hexamer of two alpha, two beta, and two delta chains.
CC   -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR   EMBL; X73824; CAA52045.1; -; Genomic_DNA.
DR   EMBL; L20472; AAC36816.1; -; Unassigned_DNA.
DR   EMBL; CP000117; ABA23632.1; -; Genomic_DNA.
DR   PIR; B36936; B36936.
DR   AlphaFoldDB; Q57302; -.
DR   SMR; Q57302; -.
DR   STRING; 240292.Ava_4027; -.
DR   EnsemblBacteria; ABA23632; ABA23632; Ava_4027.
DR   KEGG; ava:Ava_4027; -.
DR   eggNOG; COG2710; Bacteria.
DR   HOGENOM; CLU_025876_2_0_3; -.
DR   BioCyc; MetaCyc:MON-16482; -.
DR   Proteomes; UP000002533; Chromosome.
DR   GO; GO:0016613; C:vanadium-iron nitrogenase complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0018697; F:carbonyl sulfide nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016163; F:nitrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   InterPro; IPR000510; Nase/OxRdtase_comp1.
DR   InterPro; IPR000318; Nase_comp1_CS.
DR   InterPro; IPR014281; Nase_VnfK.
DR   Pfam; PF00148; Oxidored_nitro; 1.
DR   TIGRFAMs; TIGR02932; vnfK_nitrog; 1.
DR   PROSITE; PS00699; NITROGENASE_1_1; 1.
DR   PROSITE; PS00090; NITROGENASE_1_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Iron; Iron-sulfur; Metal-binding; Nitrogen fixation;
KW   Nucleotide-binding; Oxidoreductase.
FT   CHAIN           1..463
FT                   /note="Nitrogenase vanadium-iron protein beta chain"
FT                   /id="PRO_0000153086"
FT   BINDING         20
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         45
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         104
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
FT   BINDING         142
FT                   /ligand="[8Fe-7S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:21143"
FT                   /ligand_note="ligand shared with alpha chain"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        64
FT                   /note="A -> V (in Ref. 1; CAA52045/AAC36816)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   463 AA;  50805 MW;  7724491A86F1C4E8 CRC64;
     MTLAVNKKER AGVINPIFTC QPAGAEYATI GVKDCIPLVH GGQGCSMFVR LIFAQHLKEN
     FDIASSSLHE ASAVFGGMPR IEEGVKTLVA RYPDLRLIPI ITTCSTETIG DDVEGTINKV
     NKFLKKEYPN REVKLIPVHT PSYRGSQVTG YDAGVTSLIT NLAKKGEPNG KLNIITGWVN
     PGDVTEVKHI LSEMGVDANI LLDTETFNAP TMPDKNSFTF GNTTIEDIAG SANAIGTIAL
     CKYEGGNAAK FLQEQFDVPA IVGPTPIGIK NTDAWLQNIK KLTGKPIPES LVVERGKAID
     SLADLAHMYF ANKRVAIYGD PDLVIGLAEF CLEVELEPVL LLLGDDNQAA SKDPRLAELA
     KRANHAEYDI DVIWNADLWE LESRVKEKGD IDLILGHSKG RYIAIDNKIP MVRVGFPTFD
     RAGLWKNPVI GYRGAEWLGD AIANAMFADM EYKHDREWIL NVW