VNNL2_DROME
ID VNNL2_DROME Reviewed; 525 AA.
AC Q8IRR1; D5SHR9; X2JDW2;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 12-AUG-2020, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Vanin-like protein 2 {ECO:0000312|FlyBase:FBgn0052751};
DE EC=3.5.1.- {ECO:0000305};
DE Flags: Precursor;
GN ORFNames=CG32751 {ECO:0000312|FlyBase:FBgn0052751},
GN CG3648 {ECO:0000312|FlyBase:FBgn0052751};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAN09160.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:ADG03455.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=25387828; DOI=10.1534/g3.114.015040;
RA Logan-Garbisch T., Bortolazzo A., Luu P., Ford A., Do D., Khodabakhshi P.,
RA French R.L.;
RT "Developmental ethanol exposure leads to dysregulation of lipid metabolism
RT and oxidative stress in Drosophila.";
RL G3 (Bethesda) 5:49-59(2014).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B {ECO:0000312|FlyBase:FBgn0052751};
CC IsoId=Q8IRR1-1; Sequence=Displayed;
CC Name=A {ECO:0000312|FlyBase:FBgn0052751};
CC IsoId=Q8IRR1-2; Sequence=VSP_060649;
CC -!- TISSUE SPECIFICITY: Expressed in third instar larvae.
CC {ECO:0000269|PubMed:25387828}.
CC -!- INDUCTION: Induced by ethanol. {ECO:0000269|PubMed:25387828}.
CC -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC BTD/VNN family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ADG03455.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAN09160.1; -; Genomic_DNA.
DR EMBL; AE014298; AHN59398.1; -; Genomic_DNA.
DR EMBL; BT124870; ADG03455.1; ALT_INIT; mRNA.
DR RefSeq; NP_001284927.1; NM_001297998.1. [Q8IRR1-1]
DR RefSeq; NP_727068.1; NM_167061.3. [Q8IRR1-2]
DR AlphaFoldDB; Q8IRR1; -.
DR SMR; Q8IRR1; -.
DR STRING; 7227.FBpp0070845; -.
DR GlyGen; Q8IRR1; 9 sites.
DR PaxDb; Q8IRR1; -.
DR EnsemblMetazoa; FBtr0070880; FBpp0070845; FBgn0052751. [Q8IRR1-2]
DR EnsemblMetazoa; FBtr0340669; FBpp0309511; FBgn0052751. [Q8IRR1-1]
DR GeneID; 318189; -.
DR KEGG; dme:Dmel_CG32751; -.
DR UCSC; CG32751-RA; d. melanogaster. [Q8IRR1-1]
DR FlyBase; FBgn0052751; CG32751.
DR VEuPathDB; VectorBase:FBgn0052751; -.
DR eggNOG; KOG0806; Eukaryota.
DR GeneTree; ENSGT00390000013823; -.
DR HOGENOM; CLU_033209_1_0_1; -.
DR InParanoid; Q8IRR1; -.
DR OMA; YEKDWAS; -.
DR OrthoDB; 1276751at2759; -.
DR PhylomeDB; Q8IRR1; -.
DR BioGRID-ORCS; 318189; 0 hits in 3 CRISPR screens.
DR ChiTaRS; vanin-like; fly.
DR GenomeRNAi; 318189; -.
DR PRO; PR:Q8IRR1; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0052751; Expressed in spermathecum and 3 other tissues.
DR ExpressionAtlas; Q8IRR1; baseline and differential.
DR Genevisible; Q8IRR1; DM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR CDD; cd07567; biotinidase_like; 1.
DR Gene3D; 3.60.110.10; -; 1.
DR InterPro; IPR012101; Biotinidase-like_euk.
DR InterPro; IPR040154; Biotinidase/VNN.
DR InterPro; IPR003010; C-N_Hydrolase.
DR InterPro; IPR036526; C-N_Hydrolase_sf.
DR InterPro; IPR043957; Vanin_C.
DR PANTHER; PTHR10609; PTHR10609; 1.
DR Pfam; PF00795; CN_hydrolase; 1.
DR Pfam; PF19018; Vanin_C; 1.
DR PIRSF; PIRSF011861; Biotinidase; 1.
DR SUPFAM; SSF56317; SSF56317; 1.
DR PROSITE; PS50263; CN_HYDROLASE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Hydrolase; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..525
FT /note="Vanin-like protein 2"
FT /id="PRO_0000019725"
FT DOMAIN 33..303
FT /note="CN hydrolase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT ACT_SITE 72
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT ACT_SITE 167
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT ACT_SITE 199
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT CARBOHYD 20
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 99
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 116
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 176
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 333
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 348
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 375
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 235..242
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_060649"
SQ SEQUENCE 525 AA; 59549 MW; 8B1DE2AA737856D2 CRC64;
MAKNYWGFFL FCLALGLMLN LSQQASLFKR DFYTAGVVEF EPSNELSDNL AGYLEIIQSQ
NATSTDIIVF PESTLNSAGS TTFVPNPEDQ INPCLSDPNA TYYEEFLVTL SCAARNASKY
IVINLTEKQK CEDIPEDTRP CASNGLNVFN TNVVFDRQGV VVSRYRKVHL YGEPKNSTFL
PELSTFETDF GVTFGHFICF DILFYTPAHQ LIVEQGITDF VYPAMWFSQL PFLTVNGIFS
SKAVQIQLGW SYANNVNLLA AGASDPIVGN SGSGIYHGRS GSLRSVMRQE SGERSIYVAR
VPKYRAKRRM KRDLKRQVAT SSSFNIKRDY LENFTSEELK IDAGKIGNLS QNLCHGGFCC
HFDLAWRSLG KPSRNTSHYS YRVGIYEGWR NEKRLDVNYI RNCGVFTCSG NSIDDCGQLL
PDIQRPLVTF THVEIRVTYP QSREFLLFPD TLLDNLLPLE PKQFEWSQKR ISEESHQVRF
ALRKSLEVKH LLTFGIYGNY YNNECTFGVG TAEEQLECGY KNPKI
