ID   VNP_PSEPC               Reviewed;          37 AA.
AC   P82972;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Natriuretic peptide PNP;
OS   Pseudocerastes persicus (Persian horned viper) (False horned viper).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Viperinae; Pseudocerastes.
OX   NCBI_TaxID=47769;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, MASS SPECTROMETRY, AND DISULFIDE BOND.
RC   TISSUE=Venom {ECO:0000269|PubMed:14741349};
RX   PubMed=14741349; DOI=10.1016/s0014-5793(03)01455-8;
RA   Amininasab M., Elmi M.M., Endlich N., Endlich K., Parekh N.,
RA   Naderi-Manesh H., Schaller J., Mostafavi H., Sattler M., Sarbolouki M.N.,
RA   Muhle-Goll C.;
RT   "Functional and structural characterization of a novel member of the
RT   natriuretic family of peptides from the venom of Pseudocerastes persicus.";
RL   FEBS Lett. 557:104-108(2004).
RN   [2]
RP   MUTAGENESIS OF GLY-16; LYS-18; ARG-21; GLY-23 AND 24-SER--SER-26, AND
RP   STRUCTURE BY NMR.
RX   PubMed=16515458; DOI=10.2174/092986606775338452;
RA   Elmi M.M., Amininasab M., Hondo T., Kikuchi J., Kuroda Y.,
RA   Naderi-Manesh H., Sarbolouki M.N.;
RT   "Structural and functional characterization of a mutant of Pseudocerastes
RT   persicus natriuretic peptide.";
RL   Protein Pept. Lett. 13:295-300(2006).
CC   -!- FUNCTION: Increases urine flow and decreases blood pressure when
CC       administered to rats by intravenous injection. Inhibits thrombin-
CC       induced platelet aggregation. Stimulates cGMP production via the
CC       natriuretic peptide receptor-A (NPR1). {ECO:0000269|PubMed:14741349}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:14741349}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:14741349}.
CC   -!- MASS SPECTROMETRY: Mass=3941.5; Mass_error=0.17; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:14741349};
CC   -!- SIMILARITY: Belongs to the natriuretic peptide family. {ECO:0000255}.
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DR   AlphaFoldDB; P82972; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; IEA:InterPro.
DR   GO; GO:0048018; F:receptor ligand activity; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0044496; P:negative regulation of blood pressure in another organism; IDA:UniProtKB.
DR   GO; GO:0044505; P:positive regulation of G protein-coupled receptor activity in another organism; IDA:UniProtKB.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR   InterPro; IPR000663; Natr_peptide.
DR   InterPro; IPR030480; Natr_peptide_CS.
DR   InterPro; IPR002408; Natriuretic_peptide_brain.
DR   Pfam; PF00212; ANP; 1.
DR   PRINTS; PR00712; BNATPEPTIDE.
DR   PRINTS; PR00710; NATPEPTIDES.
DR   SMART; SM00183; NAT_PEP; 1.
DR   PROSITE; PS00263; NATRIURETIC_PEPTIDE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Hypotensive agent; Secreted;
KW   Toxin; Vasoactive; Vasodilator.
FT   PEPTIDE         1..37
FT                   /note="Natriuretic peptide PNP"
FT                   /id="PRO_0000045077"
FT   DISULFID        14..30
FT                   /evidence="ECO:0000269|PubMed:14741349"
FT   MUTAGEN         16
FT                   /note="G->T: Does not affect blood pressure or diuresis;
FT                   when associated with S-18; S-21; R-23 and 24-S--S-26 Del."
FT                   /evidence="ECO:0000269|PubMed:16515458"
FT   MUTAGEN         18
FT                   /note="K->S: Does not affect blood pressure or diuresis;
FT                   when associated with T-16; S-21; R-23 and 24-S--S-26 Del."
FT                   /evidence="ECO:0000269|PubMed:16515458"
FT   MUTAGEN         21
FT                   /note="R->S: Does not affect blood pressure or diuresis;
FT                   when associated with T-16; S-18; R-23 and 24-S--S-26 Del."
FT                   /evidence="ECO:0000269|PubMed:16515458"
FT   MUTAGEN         23
FT                   /note="G->R: Does not affect blood pressure or diuresis;
FT                   when associated with T-16; S-18; S-21 and 24-S--S-26 Del."
FT                   /evidence="ECO:0000269|PubMed:16515458"
FT   MUTAGEN         24..26
FT                   /note="Missing: Does not affect blood pressure or diuresis;
FT                   when associated with T-16; S-18; S-21 and R-23."
FT                   /evidence="ECO:0000269|PubMed:16515458"
SQ   SEQUENCE   37 AA;  3944 MW;  AD7BE649AD480BA6 CRC64;
     GENEPPKKKA PDGCFGHKID RIGSHSGLGC NKFKPGH