VOPP1_HUMAN
ID VOPP1_HUMAN Reviewed; 172 AA.
AC Q96AW1; B0AZU1; B2RAT4; B3KS72; C9JWR3; Q6FIE3; Q8NBN8; Q96RE5; Q9H0W4;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Vesicular, overexpressed in cancer, prosurvival protein 1;
DE AltName: Full=EGFR-coamplified and overexpressed protein;
DE Short=ECop;
DE AltName: Full=Glioblastoma-amplified secreted protein;
DE AltName: Full=Putative NF-kappa-B-activating protein 055N;
DE Flags: Precursor;
GN Name=VOPP1; Synonyms=ECOP, GASP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lung fibroblast;
RX PubMed=12761501; DOI=10.1038/sj.onc.1206406;
RA Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y.,
RA Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.;
RT "Large-scale identification and characterization of human genes that
RT activate NF-kappaB and MAPK signaling pathways.";
RL Oncogene 22:3307-3318(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=Spleen, Synovial cell, and Umbilical cord blood;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 14-172 (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=11916499; DOI=10.1093/neuonc/4.2.86;
RA Eley G.D., Reiter J.L., Pandita A., Park S., Jenkins R.B., Maihle N.J.,
RA James C.D.;
RT "A chromosomal region 7p11.2 transcript map: its development and
RT application to the study of EGFR amplicons in glioblastoma.";
RL Neuro-oncol. 4:86-94(2002).
RN [10]
RP FUNCTION.
RX PubMed=15735698; DOI=10.1038/sj.onc.1208496;
RA Park S., James C.D.;
RT "ECop (EGFR-coamplified and overexpressed protein), a novel protein,
RT regulates NF-kappaB transcriptional activity and associated apoptotic
RT response in an IkappaBalpha-dependent manner.";
RL Oncogene 24:2495-2502(2005).
RN [11]
RP SUBCELLULAR LOCATION.
RX PubMed=20571887; DOI=10.1007/s10735-010-9272-8;
RA Baras A., Moskaluk C.A.;
RT "Intracellular localization of GASP/ECOP/VOPP1.";
RL J. Mol. Histol. 41:153-164(2010).
CC -!- FUNCTION: Increases the transcriptional activity of NFKB1 by
CC facilitating its nuclear translocation, DNA-binding and associated
CC apoptotic response, when overexpressed. {ECO:0000269|PubMed:15735698}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC {ECO:0000269|PubMed:20571887}; Single-pass type I membrane protein
CC {ECO:0000269|PubMed:20571887}. Note=When overexpressed, localizes in
CC the nucleus and perinuclear regions.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q96AW1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96AW1-2; Sequence=VSP_043868;
CC Name=3;
CC IsoId=Q96AW1-3; Sequence=VSP_043867;
CC Name=4;
CC IsoId=Q96AW1-4; Sequence=VSP_055723;
CC -!- TISSUE SPECIFICITY: Widely expressed with highest levels in thymus and
CC ovary. {ECO:0000269|PubMed:11916499}.
CC -!- SIMILARITY: Belongs to the VOPP1/ECOP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK82888.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
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DR EMBL; AL136610; CAB66545.1; -; mRNA.
DR EMBL; AB097004; BAC77357.1; -; mRNA.
DR EMBL; AK075391; BAC11590.1; -; mRNA.
DR EMBL; AK092992; BAG52634.1; -; mRNA.
DR EMBL; AK314338; BAG36981.1; -; mRNA.
DR EMBL; AK315882; BAF98773.1; -; mRNA.
DR EMBL; CR533483; CAG38514.1; -; mRNA.
DR EMBL; AC073237; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC073347; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC099681; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH236957; EAL23811.1; -; Genomic_DNA.
DR EMBL; CH471201; EAW50969.1; -; Genomic_DNA.
DR EMBL; BC016650; AAH16650.1; -; mRNA.
DR EMBL; AF395824; AAK82888.1; ALT_SEQ; mRNA.
DR CCDS; CCDS47588.1; -. [Q96AW1-1]
DR CCDS; CCDS64654.1; -. [Q96AW1-2]
DR CCDS; CCDS64655.1; -. [Q96AW1-3]
DR CCDS; CCDS64656.1; -. [Q96AW1-4]
DR RefSeq; NP_001271211.1; NM_001284282.1. [Q96AW1-4]
DR RefSeq; NP_001271212.1; NM_001284283.1. [Q96AW1-3]
DR RefSeq; NP_001271213.1; NM_001284284.1. [Q96AW1-2]
DR RefSeq; NP_001308178.1; NM_001321249.1.
DR RefSeq; NP_001308179.1; NM_001321250.1.
DR RefSeq; NP_001308180.1; NM_001321251.1.
DR RefSeq; NP_110423.3; NM_030796.4. [Q96AW1-1]
DR AlphaFoldDB; Q96AW1; -.
DR BioGRID; 123516; 8.
DR IntAct; Q96AW1; 17.
DR STRING; 9606.ENSP00000285279; -.
DR iPTMnet; Q96AW1; -.
DR PhosphoSitePlus; Q96AW1; -.
DR BioMuta; VOPP1; -.
DR DMDM; 74760714; -.
DR MassIVE; Q96AW1; -.
DR PaxDb; Q96AW1; -.
DR PeptideAtlas; Q96AW1; -.
DR PRIDE; Q96AW1; -.
DR ProteomicsDB; 12027; -.
DR ProteomicsDB; 76001; -. [Q96AW1-1]
DR ProteomicsDB; 76002; -. [Q96AW1-2]
DR ProteomicsDB; 76003; -. [Q96AW1-3]
DR Antibodypedia; 27923; 132 antibodies from 20 providers.
DR DNASU; 81552; -.
DR Ensembl; ENST00000285279.10; ENSP00000285279.5; ENSG00000154978.13. [Q96AW1-1]
DR Ensembl; ENST00000418904.5; ENSP00000393210.1; ENSG00000154978.13. [Q96AW1-4]
DR Ensembl; ENST00000433959.5; ENSP00000415230.1; ENSG00000154978.13. [Q96AW1-2]
DR Ensembl; ENST00000625836.2; ENSP00000486983.1; ENSG00000154978.13. [Q96AW1-3]
DR GeneID; 81552; -.
DR KEGG; hsa:81552; -.
DR MANE-Select; ENST00000285279.10; ENSP00000285279.5; NM_030796.5; NP_110423.3.
DR UCSC; uc003tqq.5; human. [Q96AW1-1]
DR CTD; 81552; -.
DR DisGeNET; 81552; -.
DR GeneCards; VOPP1; -.
DR HGNC; HGNC:34518; VOPP1.
DR HPA; ENSG00000154978; Tissue enhanced (retina).
DR MIM; 611915; gene.
DR neXtProt; NX_Q96AW1; -.
DR OpenTargets; ENSG00000154978; -.
DR PharmGKB; PA165618503; -.
DR VEuPathDB; HostDB:ENSG00000154978; -.
DR eggNOG; ENOG502RYIF; Eukaryota.
DR GeneTree; ENSGT00390000015821; -.
DR HOGENOM; CLU_1694909_0_0_1; -.
DR InParanoid; Q96AW1; -.
DR OMA; VECIEAK; -.
DR OrthoDB; 1458369at2759; -.
DR PhylomeDB; Q96AW1; -.
DR TreeFam; TF332098; -.
DR PathwayCommons; Q96AW1; -.
DR SignaLink; Q96AW1; -.
DR BioGRID-ORCS; 81552; 52 hits in 1069 CRISPR screens.
DR ChiTaRS; VOPP1; human.
DR GeneWiki; ECOP; -.
DR GenomeRNAi; 81552; -.
DR Pharos; Q96AW1; Tbio.
DR PRO; PR:Q96AW1; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q96AW1; protein.
DR Bgee; ENSG00000154978; Expressed in thymus and 209 other tissues.
DR ExpressionAtlas; Q96AW1; baseline and differential.
DR Genevisible; Q96AW1; HS.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IDA:UniProtKB.
DR GO; GO:0005768; C:endosome; IDA:LIFEdb.
DR GO; GO:0031301; C:integral component of organelle membrane; IDA:UniProtKB.
DR InterPro; IPR026229; VOPP1.
DR PANTHER; PTHR14971; PTHR14971; 1.
DR PRINTS; PR02068; VOPPROTEIN1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasmic vesicle; Membrane; Reference proteome;
KW Signal; Transcription; Transcription regulation; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..172
FT /note="Vesicular, overexpressed in cancer, prosurvival
FT protein 1"
FT /id="PRO_0000325917"
FT TOPO_DOM 23..60
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 82..172
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 102..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..123
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..18
FT /note="MRRQPAKVAALLLGLLLE -> M (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_055723"
FT VAR_SEQ 2..18
FT /note="RRQPAKVAALLLGLLLE -> LSETLGYLSSVLLQ (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_043867"
FT VAR_SEQ 4..18
FT /note="QPAKVAALLLGLLLE -> TGSHAQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_043868"
FT CONFLICT 92
FT /note="E -> K (in Ref. 4; CAG38514)"
FT /evidence="ECO:0000305"
FT CONFLICT 111
FT /note="A -> T (in Ref. 3; BAC11590)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="M -> T (in Ref. 2; CAB66545 and 4; CAG38514)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 172 AA; 19224 MW; 7F7F75231C2A4276 CRC64;
MRRQPAKVAA LLLGLLLECT EAKKHCWYFE GLYPTYYICR SYEDCCGSRC CVRALSIQRL
WYFWFLLMMG VLFCCGAGFF IRRRMYPPPL IEEPAFNVSY TRQPPNPGPG AQQPGPPYYT
DPGGPGMNPV GNSMAMAFQV PPNSPQGSVA CPPPPAYCNT PPPPYEQVVK AK
