ID   VORA_METTH              Reviewed;         477 AA.
AC   O26801;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Ketoisovalerate oxidoreductase subunit VorA;
DE            Short=VOR;
DE            EC=1.2.7.7;
DE   AltName: Full=2-oxoisovalerate ferredoxin reductase subunit alpha;
DE   AltName: Full=2-oxoisovalerate oxidoreductase alpha chain;
GN   Name=vorA; OrderedLocusNames=MTH_705;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-methyl-2-oxobutanoate + CoA + 2 oxidized [2Fe-2S]-
CC         [ferredoxin] = 2-methylpropanoyl-CoA + CO2 + H(+) + 2 reduced [2Fe-
CC         2S]-[ferredoxin]; Xref=Rhea:RHEA:11712, Rhea:RHEA-COMP:10000,
CC         Rhea:RHEA-COMP:10001, ChEBI:CHEBI:11851, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57338; EC=1.2.7.7;
CC   -!- SUBUNIT: Heterotrimer of the VorA, VorB and VorC subunits.
CC       {ECO:0000250}.
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DR   EMBL; AE000666; AAB85210.1; -; Genomic_DNA.
DR   PIR; B69194; B69194.
DR   AlphaFoldDB; O26801; -.
DR   SMR; O26801; -.
DR   STRING; 187420.MTH_705; -.
DR   EnsemblBacteria; AAB85210; AAB85210; MTH_705.
DR   KEGG; mth:MTH_705; -.
DR   PATRIC; fig|187420.15.peg.688; -.
DR   HOGENOM; CLU_042621_0_0_2; -.
DR   OMA; RGGTSNC; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0043807; F:3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity; IEA:UniProtKB-EC.
DR   GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR   GO; GO:0006082; P:organic acid metabolic process; IEA:UniProt.
DR   GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR   GO; GO:0044272; P:sulfur compound biosynthetic process; IEA:UniProt.
DR   Gene3D; 3.40.920.10; -; 1.
DR   InterPro; IPR019752; Pyrv/ketoisovalerate_OxRed_cat.
DR   InterPro; IPR002869; Pyrv_flavodox_OxRed_cen.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR011766; TPP_enzyme-bd_C.
DR   Pfam; PF01558; POR; 1.
DR   Pfam; PF02775; TPP_enzyme_C; 1.
DR   SUPFAM; SSF52518; SSF52518; 1.
DR   SUPFAM; SSF53323; SSF53323; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..477
FT                   /note="Ketoisovalerate oxidoreductase subunit VorA"
FT                   /id="PRO_0000099949"
SQ   SEQUENCE   477 AA;  51628 MW;  FFDF19226C40790A CRC64;
     MTKKVIRKPD SLHDVFERKG GSAPTATHYC AGCGHGILHK LIGEAMDELG IQERAVMISP
     VGCAVFAYYY FDCGNVQVAH GRAPAVGTGI SRAEDDAVVI LYQGDGDLAS IGLNETIQAA
     NRGEKLAVFF VNNTVYGMTG GQMAPTTLVG EVTVTCPTGR DPRYAGYPLH MCELLDNLQA
     PVFIERVSLA DPKRIRRARR AIKRALEIQR DGKGYAFVEV LSPCPTNLRQ DAEGAERFLK
     EEMEKEFPVK NFRDRSAETE PLIRSESDFS RESLDRIFQI REDSVPDPVD DPEFPEVRVK
     IAGFGGQGVL SMGLTLAQAA CSEGRHTSWY PAYGPEQRGG TSSCGVVISG ERVGSPAVDT
     PDVLVALNQP SLDEFADDVA DGGIILYDST TASFSGGAVR AMGVPALEIA RKHGTARAAN
     TVMLGVMMAL GLTGLDEESF REAIKFTFAG KEKIIDMNLR ILEAGAEWAR ENIEGEL