VORA_PYRHO
ID VORA_PYRHO Reviewed; 394 AA.
AC O58413;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Ketoisovalerate oxidoreductase subunit VorA;
DE Short=VOR;
DE EC=1.2.7.7;
DE AltName: Full=2-oxoisovalerate ferredoxin reductase subunit alpha;
DE AltName: Full=2-oxoisovalerate oxidoreductase alpha chain;
GN Name=vorA; OrderedLocusNames=PH0680;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-methyl-2-oxobutanoate + CoA + 2 oxidized [2Fe-2S]-
CC [ferredoxin] = 2-methylpropanoyl-CoA + CO2 + H(+) + 2 reduced [2Fe-
CC 2S]-[ferredoxin]; Xref=Rhea:RHEA:11712, Rhea:RHEA-COMP:10000,
CC Rhea:RHEA-COMP:10001, ChEBI:CHEBI:11851, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57338; EC=1.2.7.7;
CC -!- SUBUNIT: Heterotetramer of one alpha, one beta, one delta and one gamma
CC chain. {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA29771.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BA000001; BAA29771.1; ALT_INIT; Genomic_DNA.
DR PIR; A71114; A71114.
DR RefSeq; WP_048053189.1; NC_000961.1.
DR AlphaFoldDB; O58413; -.
DR SMR; O58413; -.
DR IntAct; O58413; 1.
DR MINT; O58413; -.
DR STRING; 70601.3257088; -.
DR EnsemblBacteria; BAA29771; BAA29771; BAA29771.
DR GeneID; 1443008; -.
DR KEGG; pho:PH0680; -.
DR eggNOG; arCOG01608; Archaea.
DR OMA; MKSNYIS; -.
DR OrthoDB; 29908at2157; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0043807; F:3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity; IEA:UniProtKB-EC.
DR GO; GO:0006082; P:organic acid metabolic process; IEA:UniProt.
DR GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR GO; GO:0044272; P:sulfur compound biosynthetic process; IEA:UniProt.
DR CDD; cd07034; TPP_PYR_PFOR_IOR-alpha_like; 1.
DR Gene3D; 3.40.50.920; -; 1.
DR InterPro; IPR033412; PFOR_II.
DR InterPro; IPR002880; Pyrv_Fd/Flavodoxin_OxRdtase_N.
DR InterPro; IPR029061; THDP-binding.
DR InterPro; IPR009014; Transketo_C/PFOR_II.
DR Pfam; PF17147; PFOR_II; 1.
DR Pfam; PF01855; POR_N; 1.
DR SUPFAM; SSF52518; SSF52518; 1.
DR SUPFAM; SSF52922; SSF52922; 1.
PE 3: Inferred from homology;
KW Oxidoreductase.
FT CHAIN 1..394
FT /note="Ketoisovalerate oxidoreductase subunit VorA"
FT /id="PRO_0000099953"
SQ SEQUENCE 394 AA; 44126 MW; 68E5038B32D63FD3 CRC64;
MEYNPIKKVV SGNYAAAYAA LHARVQVVAA YPITPQTSII EKIAEFIANG EADIQYIPVE
SEHSAMAACI GASATGARVF TATSAQGLAL MHEMLHWAAG SRLPIVMVNV NRAMAPPWSV
WDDQTDSLSQ RDTGWMQFYA ENNQEVYDGV LMAYKVAETV NIPTMIIESA FILSHTYEVV
NMIPQELVDE FLPPRKPLYT LTDFENPIAV GALATPADYY EFRYKIAKAH EEAKKVIKSV
GKEFGERFGR DYSKMIETGY IEDADFVFMG MGSLMGTVKE AVDLLRKEGY KVGYAKVRWF
RPFPKEELLE IAESVKGIAV LDRNFSFGQE GILFTEAKGA LYNTGVRPIM KDYIVGLGGR
DFTVRDVKAI AEDMKKVVES GKLDKEIEWY HLKR
