ID   VORB_METTH              Reviewed;         352 AA.
AC   O26800;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Ketoisovalerate oxidoreductase subunit VorB;
DE            Short=VOR;
DE            EC=1.2.7.7;
DE   AltName: Full=2-oxoisovalerate ferredoxin reductase subunit beta;
DE   AltName: Full=2-oxoisovalerate oxidoreductase beta chain;
GN   Name=vorB; OrderedLocusNames=MTH_704;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-methyl-2-oxobutanoate + CoA + 2 oxidized [2Fe-2S]-
CC         [ferredoxin] = 2-methylpropanoyl-CoA + CO2 + H(+) + 2 reduced [2Fe-
CC         2S]-[ferredoxin]; Xref=Rhea:RHEA:11712, Rhea:RHEA-COMP:10000,
CC         Rhea:RHEA-COMP:10001, ChEBI:CHEBI:11851, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57338; EC=1.2.7.7;
CC   -!- SUBUNIT: Heterotrimer of the VorA, VorB and VorC subunits.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB85209.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE000666; AAB85209.1; ALT_INIT; Genomic_DNA.
DR   PIR; A69194; A69194.
DR   RefSeq; WP_048060884.1; NC_000916.1.
DR   AlphaFoldDB; O26800; -.
DR   SMR; O26800; -.
DR   STRING; 187420.MTH_704; -.
DR   EnsemblBacteria; AAB85209; AAB85209; MTH_704.
DR   GeneID; 1470665; -.
DR   KEGG; mth:MTH_704; -.
DR   PATRIC; fig|187420.15.peg.687; -.
DR   HOGENOM; CLU_017038_0_0_2; -.
DR   OMA; EAAINMV; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0043807; F:3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006082; P:organic acid metabolic process; IEA:UniProt.
DR   GO; GO:1901576; P:organic substance biosynthetic process; IEA:UniProt.
DR   GO; GO:0044272; P:sulfur compound biosynthetic process; IEA:UniProt.
DR   CDD; cd07034; TPP_PYR_PFOR_IOR-alpha_like; 1.
DR   Gene3D; 3.40.50.920; -; 1.
DR   InterPro; IPR033412; PFOR_II.
DR   InterPro; IPR002880; Pyrv_Fd/Flavodoxin_OxRdtase_N.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR009014; Transketo_C/PFOR_II.
DR   Pfam; PF17147; PFOR_II; 1.
DR   Pfam; PF01855; POR_N; 1.
DR   SUPFAM; SSF52518; SSF52518; 1.
DR   SUPFAM; SSF52922; SSF52922; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..352
FT                   /note="Ketoisovalerate oxidoreductase subunit VorB"
FT                   /id="PRO_0000099954"
SQ   SEQUENCE   352 AA;  38628 MW;  AE27D675608258A8 CRC64;
     MATQMVKGNT AVIIGAMYAG CDCYFGYPIT PASEILHEAS RYFPMVGRKF VQAESEEAAI
     NMVYGAAAAG HRVMTASSGP GISLKQEGIS FLAGAELPAV IVDVMRAGPG LGNIGPEQGD
     YNQIVKGGGH GNYRNMVLAP SSVQEMCDLT MEAFELADKY RNPVVVLTDA VLGQMAEPLR
     FPEEAVEHRP DTSWAVCGNR ETMKNLVTSI FLDFDELEEF NFYLQEKYAR IEENEVRYEE
     YLVDDAEIVM VAYGISSRVA RSAVETARAE GINVGLLRPI TLFPFPSDRI RELADGGCRF
     ISVEMSSGQM REDIRMASGC RDVELVNRMG GNLIELRDVL EKIREVAGDS SD