ID   VOSA_BEAB2              Reviewed;         273 AA.
AC   J4KR13;
DT   13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Spore development regulator vosA {ECO:0000305};
GN   Name=vosA {ECO:0000303|PubMed:26243054}; ORFNames=BBA_01023;
OS   Beauveria bassiana (strain ARSEF 2860) (White muscardine disease fungus)
OS   (Tritirachium shiotae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Cordycipitaceae; Beauveria.
OX   NCBI_TaxID=655819;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ARSEF 2860;
RX   PubMed=22761991; DOI=10.1038/srep00483;
RA   Xiao G., Ying S.-H., Zheng P., Wang Z.-L., Zhang S., Xie X.-Q., Shang Y.,
RA   St Leger R.J., Zhao G.-P., Wang C., Feng M.-G.;
RT   "Genomic perspectives on the evolution of fungal entomopathogenicity in
RT   Beauveria bassiana.";
RL   Sci. Rep. 2:483-483(2012).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RX   PubMed=26243054; DOI=10.1007/s00253-015-6823-7;
RA   Li F., Shi H.Q., Ying S.H., Feng M.G.;
RT   "WetA and VosA are distinct regulators of conidiation capacity, conidial
RT   quality, and biological control potential of a fungal insect pathogen.";
RL   Appl. Microbiol. Biotechnol. 99:10069-10081(2015).
CC   -!- FUNCTION: Component of the velB-vosA heterodimeric complex that plays a
CC       dual role in activating genes associated with spore maturation and
CC       repressing certain development-associated genes (PubMed:26243054). The
CC       complex binds DNA through the DNA-binding domain of vosA that
CC       recognizes an 11-nucleotide consensus sequence 5'-CTGGCCGCGGC-3'
CC       consisting of two motifs in the promoters of key developmental
CC       regulatory genes (By similarity). Positively regulates the expression
CC       of wetA and represses abaA and brlA (PubMed:26243054). Acts as a
CC       crucial regulator of both conidiation capacity and conidial quality
CC       (PubMed:26243054). Responsible for the synthesis and accumulation of
CC       intracellular trehalose (PubMed:26243054).
CC       {ECO:0000250|UniProtKB:Q5BBX1, ECO:0000269|PubMed:26243054}.
CC   -!- SUBUNIT: Forms a heterodimeric complex with velB; the formation of the
CC       velB-vosA complex is light-dependent (By similarity).
CC       {ECO:0000250|UniProtKB:Q5BBX1}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q5BBX1}.
CC   -!- INDUCTION: Expression is positively regulated by wetA and brlA
CC       (PubMed:26243054). {ECO:0000269|PubMed:26243054}.
CC   -!- DOMAIN: The N-terminal velvet domain contains a NF-kappa-B-like fold
CC       and is involved in DNA-binding (By similarity).
CC       {ECO:0000250|UniProtKB:Q5BBX1}.
CC   -!- DISRUPTION PHENOTYPE: Decreases expression of wetA and affects
CC       expression of abaA and brlA (PubMed:26243054). Leads to loss of
CC       conidiation capacities and defects in conidial tolerance to high
CC       osmolarity or UV-B irradiation but no change in conidial sensitivity to
CC       H(2)O(2) or the fungicide carbendazim (PubMed:26243054).
CC       {ECO:0000269|PubMed:26243054}.
CC   -!- SIMILARITY: Belongs to the velvet family. VosA subfamily.
CC       {ECO:0000305}.
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DR   EMBL; JH725151; EJP70154.1; -; Genomic_DNA.
DR   RefSeq; XP_008594342.1; XM_008596120.1.
DR   AlphaFoldDB; J4KR13; -.
DR   SMR; J4KR13; -.
DR   EnsemblFungi; EJP70154; EJP70154; BBA_01023.
DR   GeneID; 19884035; -.
DR   HOGENOM; CLU_022491_1_1_1; -.
DR   InParanoid; J4KR13; -.
DR   PHI-base; PHI:5055; -.
DR   Proteomes; UP000002762; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.3960; -; 1.
DR   InterPro; IPR021740; Velvet.
DR   InterPro; IPR037525; Velvet_dom.
DR   InterPro; IPR038491; Velvet_dom_sf.
DR   PANTHER; PTHR33572; PTHR33572; 1.
DR   Pfam; PF11754; Velvet; 2.
DR   PROSITE; PS51821; VELVET; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome; Sporulation; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..273
FT                   /note="Spore development regulator vosA"
FT                   /id="PRO_0000435917"
FT   DOMAIN          70..253
FT                   /note="Velvet"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01165"
FT   REGION          66..86
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          254..273
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           211..218
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:M2TGT8"
FT   COMPBIAS        66..80
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   273 AA;  31174 MW;  80E005E092E4DDA9 CRC64;
     MARHKTSDIM LPAAWRIPWD RQLDQHMARV IKAMAQINFA SNMHHNLFMF TTREGLLHRN
     RMLSSSTTQE LQSTQPIAVR QQPRAARACG FGDRDRRVID PPPIVQLRID GPGLSEEERR
     AYLRYPNYVM NCSIWDESGT RDASYMPDEY NHQRRLMGSL VGTPFVGDDD QNQEGCFFCF
     SDLSCRTPGA FRLKFTLIMI DPARASILRH FPTLTEIKTD IFQVYSAKEF PGMVASSSLA
     KKLKEQGCII SIKKGNERAR PRGADGRSDD EDD