ID   VOSA_MAGO7              Reviewed;         501 AA.
AC   G4NB64;
DT   13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Spore development regulator VOSA {ECO:0000305};
GN   Name=VOSA {ECO:0000303|PubMed:24632440}; ORFNames=MGG_00617;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
RN   [2]
RP   FUNCTION.
RX   PubMed=24632440; DOI=10.1016/j.fgb.2014.02.011;
RA   Kim H.J., Han J.H., Kim K.S., Lee Y.H.;
RT   "Comparative functional analysis of the velvet gene family reveals unique
RT   roles in fungal development and pathogenicity in Magnaporthe oryzae.";
RL   Fungal Genet. Biol. 66:33-43(2014).
CC   -!- FUNCTION: Component of the VELB-VOSA heterodimeric complex that plays a
CC       dual role in activating genes associated with spore maturation and
CC       repressing certain development-associated genes (By similarity). The
CC       complex binds DNA through the DNA-binding domain of VOSA that
CC       recognizes an 11-nucleotide consensus sequence 5'-CTGGCCGCGGC-3'
CC       consisting of two motifs in the promoters of key developmental
CC       regulatory genes (By similarity). Appears dispensable for the
CC       development and pathogenicity (PubMed:24632440).
CC       {ECO:0000250|UniProtKB:Q5BBX1, ECO:0000269|PubMed:24632440}.
CC   -!- SUBUNIT: Forms a heterodimeric complex with VELB; the formation of the
CC       VELB-VOSA complex is light-dependent (By similarity).
CC       {ECO:0000250|UniProtKB:Q5BBX1}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q5BBX1}.
CC   -!- DOMAIN: The N-terminal velvet domain contains a NF-kappa-B-like fold
CC       and is involved in DNA-binding (By similarity).
CC       {ECO:0000250|UniProtKB:Q5BBX1}.
CC   -!- SIMILARITY: Belongs to the velvet family. VosA subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CM001235; EHA48826.1; -; Genomic_DNA.
DR   RefSeq; XP_003718410.1; XM_003718362.1.
DR   AlphaFoldDB; G4NB64; -.
DR   SMR; G4NB64; -.
DR   STRING; 318829.MGG_00617T0; -.
DR   EnsemblFungi; MGG_00617T0; MGG_00617T0; MGG_00617.
DR   GeneID; 2674880; -.
DR   KEGG; mgr:MGG_00617; -.
DR   VEuPathDB; FungiDB:MGG_00617; -.
DR   eggNOG; ENOG502RYQD; Eukaryota.
DR   HOGENOM; CLU_544081_0_0_1; -.
DR   InParanoid; G4NB64; -.
DR   OMA; HRINTQV; -.
DR   OrthoDB; 1407766at2759; -.
DR   PHI-base; PHI:4110; -.
DR   Proteomes; UP000009058; Chromosome 5.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.3960; -; 1.
DR   InterPro; IPR021740; Velvet.
DR   InterPro; IPR037525; Velvet_dom.
DR   InterPro; IPR038491; Velvet_dom_sf.
DR   PANTHER; PTHR33572; PTHR33572; 1.
DR   Pfam; PF11754; Velvet; 2.
DR   PROSITE; PS51821; VELVET; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Sporulation; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..501
FT                   /note="Spore development regulator VOSA"
FT                   /id="PRO_0000435916"
FT   DOMAIN          52..223
FT                   /note="Velvet"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01165"
FT   REGION          26..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          67..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          228..274
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          378..455
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          474..501
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           364..371
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:M2TGT8"
FT   COMPBIAS        26..50
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        67..84
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        232..254
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        255..274
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        388..416
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        430..455
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   501 AA;  54285 MW;  551465FA0F71C2E8 CRC64;
     MSLPSIGSVG SDRMAAYMHG QQVYTGQFGD PNLTPPQAET QMSAQASAQA VGQEPEPDYK
     LVIRQEPQRA RVAQGKEKGT DRKPIDPPPI VQLIRTARSD PAQIYLQSPY YFMACTLIKG
     SDDDPDPNPN SMLGTLVSSL HKLRDLNNED GGFFIFGDLS IKIEGVFRLQ FTLFQMKDST
     CVFLDSATSQ PFTVYPQKNF EGMAESTFLT RSFSDQGVRL RVRKDSRAMT TRKRNHQHAE
     VAKKHSEWDR KQTSAVSRHS SINENDSTPT DTRGLASFAG AGSSGYYDQH RSHYGETYGH
     DTYYGGARSV KRARHEVSPG QYALPDLPQQ AYATRQPSFS DSVASMTMPP VTTAAPSLAG
     INPMSSHHGY TGSAHPGFAP HRINTQVGGS HLAPQPHSAI GSVNQGYQSP STHHQPHPHT
     AHPAGGQAYP SPSPRQSPGT APNYHYGSHH SQQTPVSLGL ETYTSAGVVG MPGPGQLVGT
     SAPSPHLGQG YRHGMINEPG A