ID   VP16_BHV1P              Reviewed;         504 AA.
AC   P30020;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   02-JUN-2021, entry version 75.
DE   RecName: Full=Tegument protein VP16 homolog;
DE   AltName: Full=Alpha trans-inducing protein;
DE   AltName: Full=Alpha-TIF;
OS   Bovine herpesvirus 1.1 (strain P8-2) (BoHV-1) (Infectious bovine
OS   rhinotracheitis virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=10324;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1327963; DOI=10.1016/0378-1119(92)90280-3;
RA   Carpenter D.E., Misra V.;
RT   "Sequences of the bovine herpesvirus 1 homologue of herpes simplex virus
RT   type-1 alpha-trans-inducing factor (UL48).";
RL   Gene 119:259-263(1992).
CC   -!- FUNCTION: Transcriptional activator of immediate-early (IE) gene
CC       products (alpha genes). Acts as a key activator of lytic infection by
CC       initiating the lytic program through the assembly of the
CC       transcriptional regulatory VP16-induced complex composed of VP16 and
CC       two cellular factors, HCFC1 and POU2F1. VP16-induced complex represents
CC       a regulatory switch: when it is on, it promotes IE-gene expression and
CC       thus lytic infection, and when it is off, it limits IE-gene
CC       transcription favoring latent infection (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: May play a role in the aggregation of tegument proteins
CC       around nucleocapsids during virus morphogenesis. {ECO:0000305}.
CC   -!- SUBUNIT: Associates with the VP16-induced complex; binding to host
CC       HCFC1 activates VP16 for association with the octamer motif-binding
CC       host protein POU2F1, to form a multiprotein-DNA complex responsible for
CC       activating transcription of the viral immediate early genes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion tegument {ECO:0000250|UniProtKB:P04486}.
CC       Host nucleus {ECO:0000250|UniProtKB:P04486}.
CC   -!- SIMILARITY: Belongs to the herpesviridae tegument protein VP16 protein
CC       family. {ECO:0000305}.
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DR   EMBL; Z11610; CAA77682.1; -; Genomic_DNA.
DR   PIR; JC1306; JC1306.
DR   SMR; P30020; -.
DR   IntAct; P30020; 1.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019033; C:viral tegument; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0039695; P:DNA-templated viral transcription; ISS:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1290.10; -; 1.
DR   InterPro; IPR003174; Alpha_TIF.
DR   InterPro; IPR036538; Alpha_TIF_sf.
DR   Pfam; PF02232; Alpha_TIF; 1.
DR   SMART; SM00814; Alpha_TIF; 1.
DR   SUPFAM; SSF56548; SSF56548; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Host nucleus; Host-virus interaction; Phosphoprotein;
KW   Transcription; Transcription regulation; Virion; Virion tegument.
FT   CHAIN           1..504
FT                   /note="Tegument protein VP16 homolog"
FT                   /id="PRO_0000115802"
FT   REGION          354..381
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          395..421
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          435..486
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   504 AA;  54029 MW;  0031B4B0E31FCD25 CRC64;
     MSGRIKTAGR ALASQCGGAA AATMDPYDAI EAFDDSLLGS PLAAGPLYDG PSPARFALPP
     PRPAPLAALL ERMQAELGFP DGPALLRAME RWNEDLFSCL PTNADLYADA ALLSADADAV
     VGAMYLAVPG DAERLDLNAH ANQPLPAPPA SEEGLPEYVA GVQAHFLAEL RAREERYAGL
     FLGYCRALLQ HLRATAARGR GAAGAGAQAD RLRQLVAARY YREASRLARL AFAHMYVATA
     REVSWRLHSQ QSQAQGVFVS LYYAWPQRRQ FTCLFHPVLF NHGVVALEDG FLDAAELRRL
     NYRRRELGLP LVRAGLVEVE VGPLVEEPPF SGSLPRALGF LNYQVRAKMG APAEAGGGWR
     RSGSTRTRGR AARSTTGRLQ RPCCGPRRRA KCCRATPRQR LRARGEPRHT SGSGAFSQGR
     RPGRVCRLGW ACKARSGPAR GGPGPSPVRS GLGLSRARGS PGPGPACGGP SRARGGRRRA
     SPANPFGGTY DALLGDRLNQ LLDF