ID   VP16_EHV1B              Reviewed;         479 AA.
AC   P28938; Q6DLJ9;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   02-DEC-2020, entry version 86.
DE   RecName: Full=Tegument protein VP16 homolog;
DE   AltName: Full=Alpha trans-inducing protein;
DE   AltName: Full=Alpha-TIF;
GN   OrderedLocusNames=12;
OS   Equine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=31520;
OH   NCBI_TaxID=9796; Equus caballus (Horse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=1318606; DOI=10.1016/0042-6822(92)90706-u;
RA   Telford E.A.R., Watson M.S., McBride K., Davison A.J.;
RT   "The DNA sequence of equine herpesvirus-1.";
RL   Virology 189:304-316(1992).
CC   -!- FUNCTION: Transcriptional activator of immediate-early (IE) gene
CC       products (alpha genes). Acts as a key activator of lytic infection by
CC       initiating the lytic program through the assembly of the
CC       transcriptional regulatory VP16-induced complex composed of VP16 and
CC       two cellular factors, HCFC1 and POU2F1. VP16-induced complex represents
CC       a regulatory switch: when it is on, it promotes IE-gene expression and
CC       thus lytic infection, and when it is off, it limits IE-gene
CC       transcription favoring latent infection (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: May play a role in the aggregation of tegument proteins
CC       around nucleocapsids during virus morphogenesis. {ECO:0000305}.
CC   -!- SUBUNIT: Associates with the VP16-induced complex; binding to host
CC       HCFC1 activates VP16 for association with the octamer motif-binding
CC       host protein POU2F1, to form a multiprotein-DNA complex responsible for
CC       activating transcription of the viral immediate early genes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion tegument {ECO:0000250|UniProtKB:P04486}.
CC       Host nucleus {ECO:0000250|UniProtKB:P04486}.
CC   -!- SIMILARITY: Belongs to the herpesviridae tegument protein VP16 protein
CC       family. {ECO:0000305}.
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DR   EMBL; AY665713; AAT67269.1; -; Genomic_DNA.
DR   PIR; D36796; IXBEA1.
DR   SMR; P28938; -.
DR   Proteomes; UP000001189; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019033; C:viral tegument; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0039695; P:DNA-templated viral transcription; ISS:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1290.10; -; 1.
DR   InterPro; IPR003174; Alpha_TIF.
DR   InterPro; IPR036538; Alpha_TIF_sf.
DR   Pfam; PF02232; Alpha_TIF; 1.
DR   SMART; SM00814; Alpha_TIF; 1.
DR   SUPFAM; SSF56548; SSF56548; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Host nucleus; Host-virus interaction; Phosphoprotein;
KW   Reference proteome; Transcription; Transcription regulation; Virion;
KW   Virion tegument.
FT   CHAIN           1..479
FT                   /note="Tegument protein VP16 homolog"
FT                   /id="PRO_0000115803"
SQ   SEQUENCE   479 AA;  53647 MW;  FF1FCCF4852568B0 CRC64;
     MCLLHISLPY LSCALLPGWY FDARPAASIV MFAAAEENDD PYPGKSGYND TCELMDMDGA
     VASFDEGMLS AIESVYSIPT KKRLALPPPK AASPGALYQR LQGELGFPEG QTLLSAMEKW
     NEDMFSALPG HVDLYTEIAL LSTSVDEVVR AGLDSLPTPS HYSPEVDLNA HGDEPFPEVP
     ALEDDLEIYV ISAQRFYLSE LRTREEHYAR LLRGYCVALL HYLYGSAKRQ LRGSGSDASL
     MHKFKQVVRD RYYREAANLA RLLYLHLYVS VTREVSWRLH ASQVINQGVF VSLHYFWAQR
     RKFECLFHPV LFNHGVVILE NDPLEFHDLQ RINYRRRELG LPLIRAGLIE EENSPLEAEP
     LFSGKLPRTI GFLTHQIRTK MEAYSDAHPA TPLFPLAEHS YSKRIGGRLS YGTTTEAMMD
     PPSPSAVLPG DPVPPLTVGV RQTAATLAIP SNLTLQSMET DGLDYSSMTG DELNQMFDI