ID   VP16_EHV4               Reviewed;         454 AA.
AC   Q00028;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   02-DEC-2020, entry version 70.
DE   RecName: Full=Tegument protein VP16 homolog;
DE   AltName: Full=Alpha trans-inducing protein;
DE   AltName: Full=Alpha-TIF;
GN   Name=12; Synonyms=B5;
OS   Equine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=10333;
OH   NCBI_TaxID=9796; Equus caballus (Horse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8259676; DOI=10.1006/viro.1994.1047;
RA   Purewal A.S., Allsopp R., Riggio M.P., Telford E.A., Azam S., Davison A.J.,
RA   Edington N.;
RT   "Equid herpesviruses 1 and 4 encode functional homologs of the herpes
RT   simplex virus type 1 virion transactivator protein, VP16.";
RL   Virology 198:385-389(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-211 AND 406-454.
RX   PubMed=1850013; DOI=10.1128/jvi.65.5.2320-2326.1991;
RA   Whittaker G.R., Riggio M.P., Halliburton I.W., Killington R.A., Allen G.P.,
RA   Meredith D.M.;
RT   "Antigenic and protein sequence homology between VP13/14, a herpes simplex
RT   virus type 1 tegument protein, and gp10, a glycoprotein of equine
RT   herpesvirus 1 and 4.";
RL   J. Virol. 65:2320-2326(1991).
CC   -!- FUNCTION: Transcriptional activator of immediate-early (IE) gene
CC       products (alpha genes). Acts as a key activator of lytic infection by
CC       initiating the lytic program through the assembly of the
CC       transcriptional regulatory VP16-induced complex composed of VP16 and
CC       two cellular factors, HCFC1 and POU2F1. VP16-induced complex represents
CC       a regulatory switch: when it is on, it promotes IE-gene expression and
CC       thus lytic infection, and when it is off, it limits IE-gene
CC       transcription favoring latent infection (By similarity). {ECO:0000250}.
CC   -!- FUNCTION: May play a role in the aggregation of tegument proteins
CC       around nucleocapsids during virus morphogenesis. {ECO:0000305}.
CC   -!- SUBUNIT: Associates with the VP16-induced complex; binding to host
CC       HCFC1 activates VP16 for association with the octamer motif-binding
CC       host protein POU2F1, to form a multiprotein-DNA complex responsible for
CC       activating transcription of the viral immediate early genes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion tegument {ECO:0000250|UniProtKB:P04486}.
CC       Host nucleus {ECO:0000250|UniProtKB:P04486}.
CC   -!- SIMILARITY: Belongs to the herpesviridae tegument protein VP16 protein
CC       family. {ECO:0000305}.
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DR   EMBL; L16590; AAA16563.1; -; Unassigned_DNA.
DR   EMBL; X17684; CAA35675.1; -; Genomic_DNA.
DR   PIR; A49285; A49285.
DR   SMR; Q00028; -.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019033; C:viral tegument; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0039695; P:DNA-templated viral transcription; ISS:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1290.10; -; 1.
DR   InterPro; IPR003174; Alpha_TIF.
DR   InterPro; IPR036538; Alpha_TIF_sf.
DR   Pfam; PF02232; Alpha_TIF; 1.
DR   SMART; SM00814; Alpha_TIF; 1.
DR   SUPFAM; SSF56548; SSF56548; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Host nucleus; Host-virus interaction; Phosphoprotein;
KW   Transcription; Transcription regulation; Virion; Virion tegument.
FT   CHAIN           1..454
FT                   /note="Tegument protein VP16 homolog"
FT                   /id="PRO_0000115804"
SQ   SEQUENCE   454 AA;  51180 MW;  8498A9A73C44B8D8 CRC64;
     MAANIAMFAD IEDYDDTRSC EYGYGTCELM DVDGVVASFD EGMLSASESI YSSPAQKRLA
     LPPPKATSPT ALYQRLQAEL GFPEGQAMLF AMEKWNEDMF SAIPVHVDLY TEIALLSTSV
     NEVVKAGLDS LPIPTNYIPE VDLNAHGSEP FPEVPALEDE LETYVISAQR FYLSELRARE
     EHYSRLLRGY CVALLHYLYG SAKRQLRGAG SDSALMHKFK QVVRDRYYRE TANLARLLYL
     HLYISVTREV SWRLHASQVV NQGIFVSLHY TWPQRRKFEC LFHPVLFNHG VVILENDPLE
     FNDLQRINYR RRELGLPLIR AGLIEEENLP LESEPTFSGK LPRTIGFLTH QIRTKMEAYS
     NAHPSTPLFP LAEHSYSKRI DGRLSYGTTA EAMMDPPSPS AVLPGDPVPP LTVGIRQTAE
     TLALPSNLTL QSMETDVLDY SSISGDELNQ MFDI